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Oral RNAi to control Drosophila suzukii: laboratory testing against larval and adult stages

(2016) JOURNAL OF PEST SCIENCE. 89(3). p.803-814
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Abstract
The spotted wing Drosophila (Drosophila suzukii) is an invasive and serious economic pest to small and stone fruits and its control is difficult. RNA interference (RNAi) or double-stranded RNA (dsRNA)-mediated gene silencing is rapidly becoming a widely used functional genomics tool in insects and holds great potential for insect pest control. This study investigates whether RNAi is functional in D. suzukii and whether oral delivery of dsRNA can elicit gene silencing and insecticidal activity. Firstly, microinjection of dsRNA targeting two essential genes (alpha COP and shrb) into the haemolymph of adult flies was performed, confirming that the RNAi system is functional and that gene silencing results in mortality. Secondly, dsRNA targeting alpha-COP and two extra essential genes, rpl13 and vha26, was mixed with artificial diet and fed to the larval and adult stages of D. suzukii. With naked dsRNA, no clear silencing and mortality were scored. However, combining dsRNA with a transfection reagent led to a significant increase in gene silencing and insect mortality. The best results were obtained with ds-Vha26. The results are discussed in relation to future optimization of the production, formulation, combinations and delivery of dsRNA.
Keywords
Drosophila suzukii, RNA interference, Double-stranded RNA, RNAi-based pesticide, Biopesticide, DOUBLE-STRANDED-RNA, SPOTTED WING DROSOPHILA, APHID ACYRTHOSIPHON-PISUM, CAENORHABDITIS-ELEGANS, GENE-EXPRESSION, CROP PROTECTION, INSECT PEST, INTERFERENCE, DSRNA, GENOME

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Citation

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MLA
Taning, Nji Tizi Clauvis et al. “Oral RNAi to Control Drosophila Suzukii: Laboratory Testing Against Larval and Adult Stages.” JOURNAL OF PEST SCIENCE 89.3 (2016): 803–814. Print.
APA
Taning, N. T. C., Christiaens, O., Berkvens, N., Casteels, H., Maes, M., & Smagghe, G. (2016). Oral RNAi to control Drosophila suzukii: laboratory testing against larval and adult stages. JOURNAL OF PEST SCIENCE, 89(3), 803–814.
Chicago author-date
Taning, Nji Tizi Clauvis, Olivier Christiaens, Nick Berkvens, Hans Casteels, Martine Maes, and Guy Smagghe. 2016. “Oral RNAi to Control Drosophila Suzukii: Laboratory Testing Against Larval and Adult Stages.” Journal of Pest Science 89 (3): 803–814.
Chicago author-date (all authors)
Taning, Nji Tizi Clauvis, Olivier Christiaens, Nick Berkvens, Hans Casteels, Martine Maes, and Guy Smagghe. 2016. “Oral RNAi to Control Drosophila Suzukii: Laboratory Testing Against Larval and Adult Stages.” Journal of Pest Science 89 (3): 803–814.
Vancouver
1.
Taning NTC, Christiaens O, Berkvens N, Casteels H, Maes M, Smagghe G. Oral RNAi to control Drosophila suzukii: laboratory testing against larval and adult stages. JOURNAL OF PEST SCIENCE. 2016;89(3):803–14.
IEEE
[1]
N. T. C. Taning, O. Christiaens, N. Berkvens, H. Casteels, M. Maes, and G. Smagghe, “Oral RNAi to control Drosophila suzukii: laboratory testing against larval and adult stages,” JOURNAL OF PEST SCIENCE, vol. 89, no. 3, pp. 803–814, 2016.
@article{8125658,
  abstract     = {The spotted wing Drosophila (Drosophila suzukii) is an invasive and serious economic pest to small and stone fruits and its control is difficult. RNA interference (RNAi) or double-stranded RNA (dsRNA)-mediated gene silencing is rapidly becoming a widely used functional genomics tool in insects and holds great potential for insect pest control. This study investigates whether RNAi is functional in D. suzukii and whether oral delivery of dsRNA can elicit gene silencing and insecticidal activity. Firstly, microinjection of dsRNA targeting two essential genes (alpha COP and shrb) into the haemolymph of adult flies was performed, confirming that the RNAi system is functional and that gene silencing results in mortality. Secondly, dsRNA targeting alpha-COP and two extra essential genes, rpl13 and vha26, was mixed with artificial diet and fed to the larval and adult stages of D. suzukii. With naked dsRNA, no clear silencing and mortality were scored. However, combining dsRNA with a transfection reagent led to a significant increase in gene silencing and insect mortality. The best results were obtained with ds-Vha26. The results are discussed in relation to future optimization of the production, formulation, combinations and delivery of dsRNA.},
  author       = {Taning, Nji Tizi Clauvis and Christiaens, Olivier and Berkvens, Nick and Casteels, Hans and Maes, Martine and Smagghe, Guy},
  issn         = {1612-4758},
  journal      = {JOURNAL OF PEST SCIENCE},
  keywords     = {Drosophila suzukii,RNA interference,Double-stranded RNA,RNAi-based pesticide,Biopesticide,DOUBLE-STRANDED-RNA,SPOTTED WING DROSOPHILA,APHID ACYRTHOSIPHON-PISUM,CAENORHABDITIS-ELEGANS,GENE-EXPRESSION,CROP PROTECTION,INSECT PEST,INTERFERENCE,DSRNA,GENOME},
  language     = {eng},
  number       = {3},
  pages        = {803--814},
  title        = {Oral RNAi to control Drosophila suzukii: laboratory testing against larval and adult stages},
  url          = {http://dx.doi.org/10.1007/s10340-016-0736-9},
  volume       = {89},
  year         = {2016},
}

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