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Characterization and expression profiles of five possible cytochrome P450 genes from Liposcelis entomophila (Enderlein) (Psocoptera: Liposcelididae)

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Abstract
In this study, the cDNAs of five cytochromes P450 genes (named CYP345P1, CYP358B1, CYP4FD2, CYP4CD2, and CYP6JN1) contained open reading frames from 1,500 to 1,554 nucleotides that encoded 499 to 517 amino acids were cloned from the psocid Liposcelis entomophila. They are characterized by predicted molecular weights from 57.67 to 59.64 kDa and theoretical isoelectric points of 5.57-9.07. Quantitative real-time PCR analysis showed these five genes were expressed at all tested developmental stages and higher expressions were observed in adults. CYP358B1 was expressed at higher levels in egg and adult compared to the larval stages. mRNA abundances of five genes were detected in both sexes and were relatively more abundant in adult females than in adult males. Synergism bioassay showed that the synergic ratio was 2.20 and 2.45 when insects were treated with the mixture of deltamethrin or malathion with the synergist piperonyl butoxide (PBO). Because PBO induces cytochrome P450s in some insects, this suggested to us that cytochromes P450 might participate in detoxification of these insecticides. The transcripts of the five cytochromes P450 genes in adult psocids could be induced to the highest level at 12h after the exposure to malathion. After exposure to deltamethrin, CYP358B1 reached maximum expression at 24h. The maximum expression of the other four genes occurred at 36 h. Treatments with the carbamate propoxur did not influence transcription of the cytochromes P450 gene. The induction profiles suggested that these five cytochrome P450 genes may be associated with deltamethrin and malathion metabolism in psocids.
Keywords
psocids, cytochrome P450, insecticide induction, transcriptional profiles, PBO, P450 GENES, BOSTRYCHOPHILA PSOCOPTERA, METABOLIZING-ENZYMES, GRAIN PROTECTANTS, STORED PRODUCTS, MALARIA VECTOR, RESISTANCE, PSOCIDS, INDUCTION, IDENTIFICATION

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Chicago
Li, Ting, Yan Liu, Dan-Dan Wei, Feng Shang, Guy Smagghe, Wei Dou, Jin-Jun Wang, and Guy Smagghe. 2016. “Characterization and Expression Profiles of Five Possible Cytochrome P450 Genes from Liposcelis Entomophila (Enderlein) (Psocoptera: Liposcelididae).” Archives of Insect Biochemistry and Physiology 92 (4): 259–273.
APA
Li, Ting, Liu, Y., Wei, D.-D., Shang, F., Smagghe, G., Dou, W., Wang, J.-J., et al. (2016). Characterization and expression profiles of five possible cytochrome P450 genes from Liposcelis entomophila (Enderlein) (Psocoptera: Liposcelididae). ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY, 92(4), 259–273.
Vancouver
1.
Li T, Liu Y, Wei D-D, Shang F, Smagghe G, Dou W, et al. Characterization and expression profiles of five possible cytochrome P450 genes from Liposcelis entomophila (Enderlein) (Psocoptera: Liposcelididae). ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY. 2016;92(4):259–73.
MLA
Li, Ting, Yan Liu, Dan-Dan Wei, et al. “Characterization and Expression Profiles of Five Possible Cytochrome P450 Genes from Liposcelis Entomophila (Enderlein) (Psocoptera: Liposcelididae).” ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY 92.4 (2016): 259–273. Print.
@article{8125648,
  abstract     = {In this study, the cDNAs of five cytochromes P450 genes (named CYP345P1, CYP358B1, CYP4FD2, CYP4CD2, and CYP6JN1) contained open reading frames from 1,500 to 1,554 nucleotides that encoded 499 to 517 amino acids were cloned from the psocid Liposcelis entomophila. They are characterized by predicted molecular weights from 57.67 to 59.64 kDa and theoretical isoelectric points of 5.57-9.07. Quantitative real-time PCR analysis showed these five genes were expressed at all tested developmental stages and higher expressions were observed in adults. CYP358B1 was expressed at higher levels in egg and adult compared to the larval stages. mRNA abundances of five genes were detected in both sexes and were relatively more abundant in adult females than in adult males. Synergism bioassay showed that the synergic ratio was 2.20 and 2.45 when insects were treated with the mixture of deltamethrin or malathion with the synergist piperonyl butoxide (PBO). Because PBO induces cytochrome P450s in some insects, this suggested to us that cytochromes P450 might participate in detoxification of these insecticides. The transcripts of the five cytochromes P450 genes in adult psocids could be induced to the highest level at 12h after the exposure to malathion. After exposure to deltamethrin, CYP358B1 reached maximum expression at 24h. The maximum expression of the other four genes occurred at 36 h. Treatments with the carbamate propoxur did not influence transcription of the cytochromes P450 gene. The induction profiles suggested that these five cytochrome P450 genes may be associated with deltamethrin and malathion metabolism in psocids.},
  author       = {Li, Ting and Liu, Yan and Wei, Dan-Dan and Shang, Feng and Smagghe, Guy and Dou, Wei and Wang, Jin-Jun and Smagghe, Guy},
  issn         = {0739-4462},
  journal      = {ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY},
  language     = {eng},
  number       = {4},
  pages        = {259--273},
  title        = {Characterization and expression profiles of five possible cytochrome P450 genes from Liposcelis entomophila (Enderlein) (Psocoptera: Liposcelididae)},
  url          = {http://dx.doi.org/10.1002/arch.21333},
  volume       = {92},
  year         = {2016},
}

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