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Comparison of enzyme-linked immunosorbent assay, surface plasmon resonance and biolayer interferometry for screening of deoxynivalenol in wheat and wheat dust

(2016) TOXINS. 8(4).
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Abstract
A sample preparation method was developed for the screening of deoxynivalenol (DON) in wheat and wheat dust. Extraction was carried out with water and was successful due to the polar character of DON. For detection, an enzyme-linked immunosorbent assay (ELISA) was compared to the sensor-based techniques of surface plasmon resonance (SPR) and biolayer interferometry (BLI) in terms of sensitivity, affinity and matrix effect. The matrix effects from wheat and wheat dust using SPR were too high to further use this screenings method. The preferred ELISA and BLI methods were validated according to the criteria established in Commission Regulation 519/2014/EC and Commission Decision 2002/657/EC. A small survey was executed on 16 wheat lots and their corresponding dust samples using the validated ELISA method. A linear correlation (r = 0.889) was found for the DON concentration in dust versus the DON concentration in wheat (LOD wheat: 233 g/kg, LOD wheat dust: 458 g/kg).
Keywords
deoxynivalenol, monoclonal antibodies, immunosensor, wheat dust, wheat, FUNGI, GRAIN DUST, MONOCLONAL-ANTIBODIES, MYCOTOXIN ANALYSIS, IMMUNOASSAY, AFFINITY

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Citation

Please use this url to cite or link to this publication:

Chicago
Sanders, Melanie, Daniel McPartlin, Kara Moran, Yirong Guo, Mia Eeckhout, Richard O’Kennedy, Sarah De Saeger, and Chris Maragos. 2016. “Comparison of Enzyme-linked Immunosorbent Assay, Surface Plasmon Resonance and Biolayer Interferometry for Screening of Deoxynivalenol in Wheat and Wheat Dust.” Toxins 8 (4).
APA
Sanders, Melanie, McPartlin, D., Moran, K., Guo, Y., Eeckhout, M., O’Kennedy, R., De Saeger, S., et al. (2016). Comparison of enzyme-linked immunosorbent assay, surface plasmon resonance and biolayer interferometry for screening of deoxynivalenol in wheat and wheat dust. TOXINS, 8(4).
Vancouver
1.
Sanders M, McPartlin D, Moran K, Guo Y, Eeckhout M, O’Kennedy R, et al. Comparison of enzyme-linked immunosorbent assay, surface plasmon resonance and biolayer interferometry for screening of deoxynivalenol in wheat and wheat dust. TOXINS. 2016;8(4).
MLA
Sanders, Melanie, Daniel McPartlin, Kara Moran, et al. “Comparison of Enzyme-linked Immunosorbent Assay, Surface Plasmon Resonance and Biolayer Interferometry for Screening of Deoxynivalenol in Wheat and Wheat Dust.” TOXINS 8.4 (2016): n. pag. Print.
@article{8124975,
  abstract     = {A sample preparation method was developed for the screening of deoxynivalenol (DON) in wheat and wheat dust. Extraction was carried out with water and was successful due to the polar character of DON. For detection, an enzyme-linked immunosorbent assay (ELISA) was compared to the sensor-based techniques of surface plasmon resonance (SPR) and biolayer interferometry (BLI) in terms of sensitivity, affinity and matrix effect. The matrix effects from wheat and wheat dust using SPR were too high to further use this screenings method. The preferred ELISA and BLI methods were validated according to the criteria established in Commission Regulation 519/2014/EC and Commission Decision 2002/657/EC. A small survey was executed on 16 wheat lots and their corresponding dust samples using the validated ELISA method. A linear correlation (r = 0.889) was found for the DON concentration in dust versus the DON concentration in wheat (LOD wheat: 233 g/kg, LOD wheat dust: 458 g/kg).},
  articleno    = {103},
  author       = {Sanders, Melanie and McPartlin, Daniel and Moran, Kara and Guo, Yirong and Eeckhout, Mia and O'Kennedy, Richard and De Saeger, Sarah and Maragos, Chris},
  issn         = {2072-6651},
  journal      = {TOXINS},
  keyword      = {deoxynivalenol,monoclonal antibodies,immunosensor,wheat dust,wheat,FUNGI,GRAIN DUST,MONOCLONAL-ANTIBODIES,MYCOTOXIN ANALYSIS,IMMUNOASSAY,AFFINITY},
  language     = {eng},
  number       = {4},
  pages        = {14},
  title        = {Comparison of enzyme-linked immunosorbent assay, surface plasmon resonance and biolayer interferometry for screening of deoxynivalenol in wheat and wheat dust},
  url          = {http://dx.doi.org/10.3390/toxins8040103},
  volume       = {8},
  year         = {2016},
}

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