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Cortisol directly impacts Flavobacterium columnare in vitro growth characteristics

Annelies Declercq (UGent) , Johan Aerts (UGent) , Bart Ampe, Freddy Haesebrouck (UGent) , Sarah De Saeger (UGent) and Annemie Decostere (UGent)
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Organization
Abstract
Teleost fish faced with stressful stimuli launch an endocrine stress response through activation of the hypothalamicpituitary-interrenal axis to release glucocorticoids, in particular cortisol, into the blood. For the majority of bacterial fish pathogens, stress is considered a key factor in disease outbreaks. Based upon studies in mammals, there is considerable evidence to suggest that, besides impairing the immune system, cortisol can have a direct effect on bacterial cells. Hitherto, this intriguing field of microbial endocrinology has remained largely unexplored in aquatic diseases. The present study investigated in vitro the impact of cortisol on phenotypic traits of the fresh water fish pathogen Flavobacterium columnare. Colonies obtained from the highly virulent (HV) isolates resulted in significantly larger and more spreading colonies compared to those from the low virulent (LV) isolates. High cortisol doses added displayed a direct effect on the bacterial cells and induced a significant decrease in colony size. An additional intriguing finding was the inverse relationship between cortisol concentrations added to the broth and the spreading character of colonies retrieved, with higher cortisol doses resulting in less rhizoid to rough and even smooth colony formation (the latter only in the LV trout isolate), suggesting a dose-response effect. The loss of the rhizoid appearance of the F. columnare colonies upon administration of cortisol, and hence the loss of motility, might indicate a phenotypic change to the biofilm state. These findings form the basis for further research on the impact of glucocorticoids on other virulence factors and biofilm formation of F. columnare.
Keywords
glucocorticoïds, BIOFILM FORMATION, Flavobacterium columnare, stress in fish, biofilm formation, aquatic diseases, MICROBIAL ENDOCRINOLOGY, FLEXIBACTER-COLUMNARIS, PSEUDOMONAS-AERUGINOSA, STRESS RESPONSES, RAINBOW-TROUT, FISH PATHOGEN, VIRULENCE, MOTILITY, DISEASE

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Citation

Please use this url to cite or link to this publication:

MLA
Declercq, Annelies et al. “Cortisol Directly Impacts Flavobacterium Columnare in Vitro Growth Characteristics.” VETERINARY RESEARCH 47 (2016): n. pag. Print.
APA
Declercq, A., Aerts, J., Ampe, B., Haesebrouck, F., De Saeger, S., & Decostere, A. (2016). Cortisol directly impacts Flavobacterium columnare in vitro growth characteristics. VETERINARY RESEARCH, 47.
Chicago author-date
Declercq, Annelies, Johan Aerts, Bart Ampe, Freddy Haesebrouck, Sarah De Saeger, and Annemie Decostere. 2016. “Cortisol Directly Impacts Flavobacterium Columnare in Vitro Growth Characteristics.” Veterinary Research 47.
Chicago author-date (all authors)
Declercq, Annelies, Johan Aerts, Bart Ampe, Freddy Haesebrouck, Sarah De Saeger, and Annemie Decostere. 2016. “Cortisol Directly Impacts Flavobacterium Columnare in Vitro Growth Characteristics.” Veterinary Research 47.
Vancouver
1.
Declercq A, Aerts J, Ampe B, Haesebrouck F, De Saeger S, Decostere A. Cortisol directly impacts Flavobacterium columnare in vitro growth characteristics. VETERINARY RESEARCH. 2016;47.
IEEE
[1]
A. Declercq, J. Aerts, B. Ampe, F. Haesebrouck, S. De Saeger, and A. Decostere, “Cortisol directly impacts Flavobacterium columnare in vitro growth characteristics,” VETERINARY RESEARCH, vol. 47, 2016.
@article{8124949,
  abstract     = {Teleost fish faced with stressful stimuli launch an endocrine stress response through activation of the hypothalamicpituitary-interrenal axis to release glucocorticoids, in particular cortisol, into the blood. For the majority of bacterial fish pathogens, stress is considered a key factor in disease outbreaks. Based upon studies in mammals, there is considerable evidence to suggest that, besides impairing the immune system, cortisol can have a direct effect on bacterial cells. Hitherto, this intriguing field of microbial endocrinology has remained largely unexplored in aquatic diseases. The present study investigated in vitro the impact of cortisol on phenotypic traits of the fresh water fish pathogen Flavobacterium columnare. Colonies obtained from the highly virulent (HV) isolates resulted in significantly larger and more spreading colonies compared to those from the low virulent (LV) isolates. High cortisol doses added displayed a direct effect on the bacterial cells and induced a significant decrease in colony size. An additional intriguing finding was the inverse relationship between cortisol concentrations added to the broth and the spreading character of colonies retrieved, with higher cortisol doses resulting in less rhizoid to rough and even smooth colony formation (the latter only in the LV trout isolate), suggesting a dose-response effect. The loss of the rhizoid appearance of the F. columnare colonies upon administration of cortisol, and hence the loss of motility, might indicate a phenotypic change to the biofilm state. These findings form the basis for further research on the impact of glucocorticoids on other virulence factors and biofilm formation of F. columnare.},
  articleno    = {84},
  author       = {Declercq, Annelies and Aerts, Johan and Ampe, Bart and Haesebrouck, Freddy and De Saeger, Sarah and Decostere, Annemie},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keywords     = {glucocorticoïds,BIOFILM FORMATION,Flavobacterium columnare,stress in fish,biofilm formation,aquatic diseases,MICROBIAL ENDOCRINOLOGY,FLEXIBACTER-COLUMNARIS,PSEUDOMONAS-AERUGINOSA,STRESS RESPONSES,RAINBOW-TROUT,FISH PATHOGEN,VIRULENCE,MOTILITY,DISEASE},
  language     = {eng},
  pages        = {9},
  title        = {Cortisol directly impacts Flavobacterium columnare in vitro growth characteristics},
  url          = {http://dx.doi.org/10.1186/s13567-016-0370-9},
  volume       = {47},
  year         = {2016},
}

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