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In vivo detection of reactive oxygen species and redox status in Caenorhabditis elegans

Bart Braeckman (UGent) , Arne Smolders (UGent) , Patricia Back (UGent) and Sasha De Henau (UGent)
(2016) ANTIOXIDANTS & REDOX SIGNALING. 25(10). p.577-592
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Organization
Abstract
Significance: Due to its large families of redox-active enzymes, genetic amenability, and complete transparency, the nematode Caenorhabditis elegans has the potential to become an important model for the in vivo study of redox biology. Recent Advances: The recent development of several genetically encoded ratiometric reactive oxygen species (ROS) and redox sensors has revolutionized the quantification and precise localization of ROS and redox signals in living organisms. Only few exploratory studies have applied these sensors in C. elegans and undoubtedly much remains to be discovered in this model. As a follow-up to our recent findings that the C. elegans somatic gonad uses superoxide and hydrogen peroxide (H2O2) signals to communicate with the germline, we here analyze the patterns of H2O2 inside the C. elegans germline. Critical Issues: Despite the advantages of genetically encoded ROS and redox sensors over classic chemical sensors, still several general as well as C. elegans-specific issues need to be addressed. The major concerns for the application of these sensors in C. elegans are (i) decreased vitality of some reporter strains, (ii) interference of autofluorescent compartments with the sensor signal, and (iii) the use of immobilization methods that do not influence the worm's redox physiology. Future Directions: We propose that several of the current issues may be solved by designing reporter strains carrying single copies of codon-optimized sensors. Preferably, these sensors should have their emission wavelengths in the red region, where autofluorescence is absent. Worm analysis could be optimized using four-dimensional ratiometric fluorescence microscopy of worms immobilized in microfluidic chips.
Keywords
INTRACELLULAR HYDROGEN-PEROXIDE, GREEN FLUORESCENT PROTEIN, MOLECULAR-WEIGHT THIOLS, MITOFLASH PROBE CPYFP, LIFE-SPAN, OXIDATIVE STRESS, C-ELEGANS, GENE-EXPRESSION, SUPEROXIDE DISMUTASES, PEROXISOMAL CATALASE

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MLA
Braeckman, Bart, et al. “In Vivo Detection of Reactive Oxygen Species and Redox Status in Caenorhabditis Elegans.” ANTIOXIDANTS & REDOX SIGNALING, vol. 25, no. 10, 2016, pp. 577–92, doi:10.1089/ars.2016.6751.
APA
Braeckman, B., Smolders, A., Back, P., & De Henau, S. (2016). In vivo detection of reactive oxygen species and redox status in Caenorhabditis elegans. ANTIOXIDANTS & REDOX SIGNALING, 25(10), 577–592. https://doi.org/10.1089/ars.2016.6751
Chicago author-date
Braeckman, Bart, Arne Smolders, Patricia Back, and Sasha De Henau. 2016. “In Vivo Detection of Reactive Oxygen Species and Redox Status in Caenorhabditis Elegans.” ANTIOXIDANTS & REDOX SIGNALING 25 (10): 577–92. https://doi.org/10.1089/ars.2016.6751.
Chicago author-date (all authors)
Braeckman, Bart, Arne Smolders, Patricia Back, and Sasha De Henau. 2016. “In Vivo Detection of Reactive Oxygen Species and Redox Status in Caenorhabditis Elegans.” ANTIOXIDANTS & REDOX SIGNALING 25 (10): 577–592. doi:10.1089/ars.2016.6751.
Vancouver
1.
Braeckman B, Smolders A, Back P, De Henau S. In vivo detection of reactive oxygen species and redox status in Caenorhabditis elegans. ANTIOXIDANTS & REDOX SIGNALING. 2016;25(10):577–92.
IEEE
[1]
B. Braeckman, A. Smolders, P. Back, and S. De Henau, “In vivo detection of reactive oxygen species and redox status in Caenorhabditis elegans,” ANTIOXIDANTS & REDOX SIGNALING, vol. 25, no. 10, pp. 577–592, 2016.
@article{8122803,
  abstract     = {{Significance: Due to its large families of redox-active enzymes, genetic amenability, and complete transparency, the nematode Caenorhabditis elegans has the potential to become an important model for the in vivo study of redox biology.
Recent Advances: The recent development of several genetically encoded ratiometric reactive oxygen species (ROS) and redox sensors has revolutionized the quantification and precise localization of ROS and redox signals in living organisms. Only few exploratory studies have applied these sensors in C. elegans and undoubtedly much remains to be discovered in this model. As a follow-up to our recent findings that the C. elegans somatic gonad uses superoxide and hydrogen peroxide (H2O2) signals to communicate with the germline, we here analyze the patterns of H2O2 inside the C. elegans germline.
Critical Issues: Despite the advantages of genetically encoded ROS and redox sensors over classic chemical sensors, still several general as well as C. elegans-specific issues need to be addressed. The major concerns for the application of these sensors in C. elegans are (i) decreased vitality of some reporter strains, (ii) interference of autofluorescent compartments with the sensor signal, and (iii) the use of immobilization methods that do not influence the worm's redox physiology.
Future Directions: We propose that several of the current issues may be solved by designing reporter strains carrying single copies of codon-optimized sensors. Preferably, these sensors should have their emission wavelengths in the red region, where autofluorescence is absent. Worm analysis could be optimized using four-dimensional ratiometric fluorescence microscopy of worms immobilized in microfluidic chips.}},
  author       = {{Braeckman, Bart and Smolders, Arne and Back, Patricia and De Henau, Sasha}},
  issn         = {{1523-0864}},
  journal      = {{ANTIOXIDANTS & REDOX SIGNALING}},
  keywords     = {{INTRACELLULAR HYDROGEN-PEROXIDE,GREEN FLUORESCENT PROTEIN,MOLECULAR-WEIGHT THIOLS,MITOFLASH PROBE CPYFP,LIFE-SPAN,OXIDATIVE STRESS,C-ELEGANS,GENE-EXPRESSION,SUPEROXIDE DISMUTASES,PEROXISOMAL CATALASE}},
  language     = {{eng}},
  number       = {{10}},
  pages        = {{577--592}},
  title        = {{In vivo detection of reactive oxygen species and redox status in Caenorhabditis elegans}},
  url          = {{http://dx.doi.org/10.1089/ars.2016.6751}},
  volume       = {{25}},
  year         = {{2016}},
}

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