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Proteome-wide changes in protein turnover rates in C. elegans models of longevity and age-related disease

Marieke Visscher, Sasha De Henau, Mattheus HE Wildschut, Robert M van Es, Ineke Dhondt UGent, Helen Michels, Patrick Kemmeren, Ellen A Nollen, Bart Braeckman UGent, Boudewijn MT Burgering, et al. (2016) CELL REPORTS. 16(11). p.3041-3051
abstract
The balance between protein synthesis and protein breakdown is a major determinant of protein homeostasis, and loss of protein homeostasis is one of the hallmarks of aging. Here we describe pulsed SILAC-based experiments to estimate proteome-wide turnover rates of individual proteins. We applied this method to determine protein turnover rates in Caenorhabditis elegans models of longevity and Parkinson's disease, using both developing and adult animals. Whereas protein turnover in developing, long-lived daf-2(e1370) worms is about 30% slower than in controls, the opposite was observed in day 5 adult worms, in which protein turnover in the daf-2(e1370) mutant is twice as fast as in controls. In the Parkinson's model, protein turnover is reduced proportionally over the entire proteome, suggesting that the protein homeostasis network has a strong ability to adapt. The findings shed light on the relationship between protein turnover and healthy aging.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
SILAC, DYNAMICS, MUTANT, COMPLEX, METABOLISM, PROTEOSTASIS, INHIBIT TRANSLATION, LIFE-SPAN, CAENORHABDITIS-ELEGANS, MESSENGER-RNA TRANSLATION
journal title
CELL REPORTS
Cell Reports
volume
16
issue
11
pages
3041 - 3051
Web of Science type
Article
Web of Science id
000383882300023
JCR category
CELL BIOLOGY
JCR impact factor
8.282 (2016)
JCR rank
26/189 (2016)
JCR quartile
1 (2016)
ISSN
2211-1247
DOI
10.1016/j.celrep.2016.08.025
language
English
UGent publication?
yes
classification
A1
copyright statement
Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
id
8121767
handle
http://hdl.handle.net/1854/LU-8121767
date created
2016-10-19 17:17:56
date last changed
2017-07-24 13:56:14
@article{8121767,
  abstract     = {The balance between protein synthesis and protein breakdown is a major determinant of protein homeostasis, and loss of protein homeostasis is one of the hallmarks of aging. Here we describe pulsed SILAC-based experiments to estimate proteome-wide turnover rates of individual proteins. We applied this method to determine protein turnover rates in Caenorhabditis elegans models of longevity and Parkinson's disease, using both developing and adult animals. Whereas protein turnover in developing, long-lived daf-2(e1370) worms is about 30\% slower than in controls, the opposite was observed in day 5 adult worms, in which protein turnover in the daf-2(e1370) mutant is twice as fast as in controls. In the Parkinson's model, protein turnover is reduced proportionally over the entire proteome, suggesting that the protein homeostasis network has a strong ability to adapt. The findings shed light on the relationship between protein turnover and healthy aging.},
  author       = {Visscher, Marieke and De Henau, Sasha and Wildschut, Mattheus HE and van Es, Robert M and Dhondt, Ineke and Michels, Helen and Kemmeren, Patrick and Nollen, Ellen A and Braeckman, Bart and Burgering, Boudewijn MT and Vos, Harmjan R and Dansen, Tobias B},
  issn         = {2211-1247},
  journal      = {CELL REPORTS},
  keyword      = {SILAC,DYNAMICS,MUTANT,COMPLEX,METABOLISM,PROTEOSTASIS,INHIBIT TRANSLATION,LIFE-SPAN,CAENORHABDITIS-ELEGANS,MESSENGER-RNA TRANSLATION},
  language     = {eng},
  number       = {11},
  pages        = {3041--3051},
  title        = {Proteome-wide changes in protein turnover rates in C. elegans models of longevity and age-related disease},
  url          = {http://dx.doi.org/10.1016/j.celrep.2016.08.025},
  volume       = {16},
  year         = {2016},
}

Chicago
Visscher, Marieke, Sasha De Henau, Mattheus HE Wildschut, Robert M van Es, Ineke Dhondt, Helen Michels, Patrick Kemmeren, et al. 2016. “Proteome-wide Changes in Protein Turnover Rates in C. Elegans Models of Longevity and Age-related Disease.” Cell Reports 16 (11): 3041–3051.
APA
Visscher, Marieke, De Henau, S., Wildschut, M. H., van Es, R. M., Dhondt, I., Michels, H., Kemmeren, P., et al. (2016). Proteome-wide changes in protein turnover rates in C. elegans models of longevity and age-related disease. CELL REPORTS, 16(11), 3041–3051.
Vancouver
1.
Visscher M, De Henau S, Wildschut MH, van Es RM, Dhondt I, Michels H, et al. Proteome-wide changes in protein turnover rates in C. elegans models of longevity and age-related disease. CELL REPORTS. 2016;16(11):3041–51.
MLA
Visscher, Marieke, Sasha De Henau, Mattheus HE Wildschut, et al. “Proteome-wide Changes in Protein Turnover Rates in C. Elegans Models of Longevity and Age-related Disease.” CELL REPORTS 16.11 (2016): 3041–3051. Print.