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Mitochondrial and chloroplast stress responses are modulated in distinct touch and chemical inhibition phases

(2016) PLANT PHYSIOLOGY. 171(3). p.2150-2165
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Abstract
Previous studies have identified a range of transcription factors that modulate retrograde regulation of mitochondrial and chloroplast functions in Arabidopsis (Arabidopsis thaliana). However, the relative importance of these regulators and whether they act downstream of separate or overlapping signaling cascades is still unclear. Here, we demonstrate that multiple stress-related signaling pathways, with distinct kinetic signatures, converge on overlapping gene sets involved in energy organelle function. The transcription factor ANAC017 is almost solely responsible for transcript induction of marker genes around 3 to 6 h after chemical inhibition of organelle function and is a key regulator of mitochondrial and specific types of chloroplast retrograde signaling. However, an independent and highly transient gene expression phase, initiated within 10 to 30 min after treatment, also targets energy organelle functions, and is related to touch and wounding responses. Metabolite analysis demonstrates that this early response is concurrent with rapid changes in tricarboxylic acid cycle intermediates and large changes in transcript abundance of genes encoding mitochondrial dicarboxylate carrier proteins. It was further demonstrated that transcription factors AtWRKY15 and AtWRKY40 have repressive regulatory roles in this touch-responsive gene expression. Together, our results show that several regulatory systems can independently affect energy organelle function in response to stress, providing different means to exert operational control.
Keywords
OSMOTIC-STRESS, EXPRESSION, ALTERNATIVE OXIDASE, RETROGRADE REGULATION, ARABIDOPSIS-THALIANA, GENES-ENCODING MITOCHONDRIAL, NAC TRANSCRIPTION FACTOR, PROTEINS, PLANTS, IDENTIFICATION

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MLA
Van Aken, Olivier et al. “Mitochondrial and Chloroplast Stress Responses Are Modulated in Distinct Touch and Chemical Inhibition Phases.” PLANT PHYSIOLOGY 171.3 (2016): 2150–2165. Print.
APA
Van Aken, O., De Clercq, I., Ivanova, A., Law, S. R., Van Breusegem, F., Millar, A. H., & Whelan, J. (2016). Mitochondrial and chloroplast stress responses are modulated in distinct touch and chemical inhibition phases. PLANT PHYSIOLOGY, 171(3), 2150–2165.
Chicago author-date
Van Aken, Olivier, Inge De Clercq, Aneta Ivanova, Simon R Law, Frank Van Breusegem, A Harvey Millar, and James Whelan. 2016. “Mitochondrial and Chloroplast Stress Responses Are Modulated in Distinct Touch and Chemical Inhibition Phases.” Plant Physiology 171 (3): 2150–2165.
Chicago author-date (all authors)
Van Aken, Olivier, Inge De Clercq, Aneta Ivanova, Simon R Law, Frank Van Breusegem, A Harvey Millar, and James Whelan. 2016. “Mitochondrial and Chloroplast Stress Responses Are Modulated in Distinct Touch and Chemical Inhibition Phases.” Plant Physiology 171 (3): 2150–2165.
Vancouver
1.
Van Aken O, De Clercq I, Ivanova A, Law SR, Van Breusegem F, Millar AH, et al. Mitochondrial and chloroplast stress responses are modulated in distinct touch and chemical inhibition phases. PLANT PHYSIOLOGY. 2016;171(3):2150–65.
IEEE
[1]
O. Van Aken et al., “Mitochondrial and chloroplast stress responses are modulated in distinct touch and chemical inhibition phases,” PLANT PHYSIOLOGY, vol. 171, no. 3, pp. 2150–2165, 2016.
@article{8113219,
  abstract     = {{Previous studies have identified a range of transcription factors that modulate retrograde regulation of mitochondrial and chloroplast functions in Arabidopsis (Arabidopsis thaliana). However, the relative importance of these regulators and whether they act downstream of separate or overlapping signaling cascades is still unclear. Here, we demonstrate that multiple stress-related signaling pathways, with distinct kinetic signatures, converge on overlapping gene sets involved in energy organelle function. The transcription factor ANAC017 is almost solely responsible for transcript induction of marker genes around 3 to 6 h after chemical inhibition of organelle function and is a key regulator of mitochondrial and specific types of chloroplast retrograde signaling. However, an independent and highly transient gene expression phase, initiated within 10 to 30 min after treatment, also targets energy organelle functions, and is related to touch and wounding responses. Metabolite analysis demonstrates that this early response is concurrent with rapid changes in tricarboxylic acid cycle intermediates and large changes in transcript abundance of genes encoding mitochondrial dicarboxylate carrier proteins. It was further demonstrated that transcription factors AtWRKY15 and AtWRKY40 have repressive regulatory roles in this touch-responsive gene expression. Together, our results show that several regulatory systems can independently affect energy organelle function in response to stress, providing different means to exert operational control.}},
  author       = {{Van Aken, Olivier and De Clercq, Inge and Ivanova, Aneta and Law, Simon R and Van Breusegem, Frank and Millar, A Harvey and Whelan, James}},
  issn         = {{0032-0889}},
  journal      = {{PLANT PHYSIOLOGY}},
  keywords     = {{OSMOTIC-STRESS,EXPRESSION,ALTERNATIVE OXIDASE,RETROGRADE REGULATION,ARABIDOPSIS-THALIANA,GENES-ENCODING MITOCHONDRIAL,NAC TRANSCRIPTION FACTOR,PROTEINS,PLANTS,IDENTIFICATION}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{2150--2165}},
  title        = {{Mitochondrial and chloroplast stress responses are modulated in distinct touch and chemical inhibition phases}},
  url          = {{http://dx.doi.org/10.1104/pp.16.00273}},
  volume       = {{171}},
  year         = {{2016}},
}

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