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Comparative chemical screening and genetic analysis reveal tentoxin as a new virulence factor in Cochliobolus miyabeanus, the causal agent of brown spot disease on rice

(2016) MOLECULAR PLANT PATHOLOGY. 17(6). p.805-817
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Abstract
Brown spot disease, caused by Cochliobolus miyabeanus, is currently considered to be one of the most important yield reducers of rice (Oryza sativaL.). Despite its agricultural importance, little is known about the virulence mechanisms deployed by the fungus. Therefore, we set out to identify novel virulence factors with a role in disease development. This article reports, for the first time, the production of tentoxin by C.miyabeanus as a virulence factor during brown spot disease and the identification of the non-ribosomal protein synthetase (NRPS) CmNps3, responsible for tentoxin biosynthesis. We compared the chemical compounds produced by C.miyabeanus strains differing in virulence ability using ultra-high-performance liquid chromatography (UHPLC) coupled to high-resolution Orbitrap mass spectrometry (HRMS). The production of tentoxin by a highly virulent strain was revealed by principal component analysis of the detected ions and confirmed by UHPLC coupled to tandem-quadrupole mass spectrometry (MS/MS). The corresponding NRPS was identified by insilico genome analysis and confirmed by gene deletion. Infection tests with wild-type and Cmnps3 mutants showed that tentoxin acts as a virulence factor and is correlated with chlorosis development during the second phase of infection. Although rice has previously been classified as a tentoxin-insensitive plant species, our data demonstrate that tentoxin production by C.miyabeanus affects symptom development.
Keywords
brown spot disease, Bipolaris oryzae, Cochliobolus miyabeanus, Oryza sativaL, plant-pathogen interactions, rice, tentoxin, NONRIBOSOMAL PEPTIDE SYNTHETASES, POLYPHENOL OXIDASE, ADENYLATION DOMAINS, PHYTOTOXIN TENTOXIN, ALTERNARIA TOXINS, PLANT-DISEASE, WINTER-WHEAT, T-TOXIN, HETEROSTROPHUS, SPECIFICITY

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Citation

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Chicago
De Bruyne, Lieselotte, Christof Van Poucke, José Diana Di Mavungu, Nur Ain Izzati Mohd Zainudin, Lynn Vanhaecke, David De Vleesschauwer, B Gillian Turgeon, Sarah De Saeger, and Monica Höfte. 2016. “Comparative Chemical Screening and Genetic Analysis Reveal Tentoxin as a New Virulence Factor in Cochliobolus Miyabeanus, the Causal Agent of Brown Spot Disease on Rice.” Molecular Plant Pathology 17 (6): 805–817.
APA
De Bruyne, Lieselotte, Van Poucke, C., Diana Di Mavungu, J., Zainudin, N. A. I. M., Vanhaecke, L., De Vleesschauwer, D., Turgeon, B. G., et al. (2016). Comparative chemical screening and genetic analysis reveal tentoxin as a new virulence factor in Cochliobolus miyabeanus, the causal agent of brown spot disease on rice. MOLECULAR PLANT PATHOLOGY, 17(6), 805–817.
Vancouver
1.
De Bruyne L, Van Poucke C, Diana Di Mavungu J, Zainudin NAIM, Vanhaecke L, De Vleesschauwer D, et al. Comparative chemical screening and genetic analysis reveal tentoxin as a new virulence factor in Cochliobolus miyabeanus, the causal agent of brown spot disease on rice. MOLECULAR PLANT PATHOLOGY. 2016;17(6):805–17.
MLA
De Bruyne, Lieselotte et al. “Comparative Chemical Screening and Genetic Analysis Reveal Tentoxin as a New Virulence Factor in Cochliobolus Miyabeanus, the Causal Agent of Brown Spot Disease on Rice.” MOLECULAR PLANT PATHOLOGY 17.6 (2016): 805–817. Print.
@article{8112175,
  abstract     = {Brown spot disease, caused by Cochliobolus miyabeanus, is currently considered to be one of the most important yield reducers of rice (Oryza sativaL.). Despite its agricultural importance, little is known about the virulence mechanisms deployed by the fungus. Therefore, we set out to identify novel virulence factors with a role in disease development. This article reports, for the first time, the production of tentoxin by C.miyabeanus as a virulence factor during brown spot disease and the identification of the non-ribosomal protein synthetase (NRPS) CmNps3, responsible for tentoxin biosynthesis. We compared the chemical compounds produced by C.miyabeanus strains differing in virulence ability using ultra-high-performance liquid chromatography (UHPLC) coupled to high-resolution Orbitrap mass spectrometry (HRMS). The production of tentoxin by a highly virulent strain was revealed by principal component analysis of the detected ions and confirmed by UHPLC coupled to tandem-quadrupole mass spectrometry (MS/MS). The corresponding NRPS was identified by insilico genome analysis and confirmed by gene deletion. Infection tests with wild-type and Cmnps3 mutants showed that tentoxin acts as a virulence factor and is correlated with chlorosis development during the second phase of infection. Although rice has previously been classified as a tentoxin-insensitive plant species, our data demonstrate that tentoxin production by C.miyabeanus affects symptom development.},
  author       = {De Bruyne, Lieselotte and Van Poucke, Christof and Diana Di Mavungu, José and Zainudin, Nur Ain Izzati Mohd and Vanhaecke, Lynn and De Vleesschauwer, David and Turgeon, B Gillian and De Saeger, Sarah and Höfte, Monica},
  issn         = {1464-6722},
  journal      = {MOLECULAR PLANT PATHOLOGY},
  keywords     = {brown spot disease,Bipolaris oryzae,Cochliobolus miyabeanus,Oryza sativaL,plant-pathogen interactions,rice,tentoxin,NONRIBOSOMAL PEPTIDE SYNTHETASES,POLYPHENOL OXIDASE,ADENYLATION DOMAINS,PHYTOTOXIN TENTOXIN,ALTERNARIA TOXINS,PLANT-DISEASE,WINTER-WHEAT,T-TOXIN,HETEROSTROPHUS,SPECIFICITY},
  language     = {eng},
  number       = {6},
  pages        = {805--817},
  title        = {Comparative chemical screening and genetic analysis reveal tentoxin as a new virulence factor in Cochliobolus miyabeanus, the causal agent of brown spot disease on rice},
  url          = {http://dx.doi.org/10.1111/mpp.12329},
  volume       = {17},
  year         = {2016},
}

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