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Diversity of root-lesion nematodes (Pratylenchus spp.) associated with wheat (Triticum aestivum and T. durum) in Morocco

Fouad Mokrini, Lieven Waeyenberge, Nicole Viaene UGent, Fouad Abbad Andaloussi and Maurice Moens UGent (2016) NEMATOLOGY. 18. p.781-801
abstract
Root-lesion nematodes (Pratylenchus) have a worldwide distribution and cause severe production constraints on numerous important crops. During a survey of the wheat-growing area of Morocco, 17 populations of root-lesion nematode were collected. They were identified on the basis of their morphological and morphometric characters, and by molecular methods. Microscopic observations of females and males demonstrated the occurrence of P. penetrans in 13 of the 17 samples; P. thornei and P. pseudocoffeae were detected in four samples from Zaers and a single sample from Settat, respectively. A duplex PCR primer set was used to confirm the presence of P. penetrans while the species-specific forward primer PTHO and the common reverse primer D3B were used for P. thornei. For the remaining populations, the D2-D3 expansion segments of the 28S rRNA gene were amplified and the obtained sequences were compared with those of Pratylenchus species in the GenBank database. This comparison confirmed the morphological identifications and revealed a population of P. pinguicaudatus. The study of the phylogenetic relationship of the Moroccan Pratylenchus populations showed a high similarity (99-100%) between all P. penetrans populations. The population dynamics of six Pratylenchus populations fromMorocco were evaluated on carrot disk cultures at 4, 8 and 12 weeks after inoculation, and at 10, 15, 20 and 25 degrees C. The optimum temperature for reproduction of all populations was 20 degrees C. After 8 weeks at this temperature, nematode numbers increased up to 458-fold, 310-fold and 252-fold for the four populations of P. penetrans, the P. thornei and the P. pseudocoffeae population, respectively.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
RIBOSOMAL DNA, AMMOPHILA-ARENARIA, CV POYO, POPULATIONS, REPRODUCTIVE FITNESS, PHYLOGENETIC-RELATIONSHIPS, RADOPHOLUS-SIMILIS, MOLECULAR CHARACTERIZATION, N. SP NEMATODA, PLANT-PARASITIC NEMATODES, reproductive fitness, rDNA, Pratylenchidae, phylogeny, morphometrics, morphology, D2-D3, molecular
journal title
NEMATOLOGY
Nematology
volume
18
pages
781 - 801
Web of Science type
Article
Web of Science id
000381949400003
JCR category
ZOOLOGY
JCR impact factor
1.162 (2016)
JCR rank
73/162 (2016)
JCR quartile
2 (2016)
ISSN
1388-5545
DOI
10.1163/15685411-00002993
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8111948
handle
http://hdl.handle.net/1854/LU-8111948
date created
2016-10-12 15:00:49
date last changed
2016-12-19 15:39:17
@article{8111948,
  abstract     = {Root-lesion nematodes (Pratylenchus) have a worldwide distribution and cause severe production constraints on numerous important crops. During a survey of the wheat-growing area of Morocco, 17 populations of root-lesion nematode were collected. They were identified on the basis of their morphological and morphometric characters, and by molecular methods. Microscopic observations of females and males demonstrated the occurrence of P. penetrans in 13 of the 17 samples; P. thornei and P. pseudocoffeae were detected in four samples from Zaers and a single sample from Settat, respectively. A duplex PCR primer set was used to confirm the presence of P. penetrans while the species-specific forward primer PTHO and the common reverse primer D3B were used for P. thornei. For the remaining populations, the D2-D3 expansion segments of the 28S rRNA gene were amplified and the obtained sequences were compared with those of Pratylenchus species in the GenBank database. This comparison confirmed the morphological identifications and revealed a population of P. pinguicaudatus. The study of the phylogenetic relationship of the Moroccan Pratylenchus populations showed a high similarity (99-100\%) between all P. penetrans populations. The population dynamics of six Pratylenchus populations fromMorocco were evaluated on carrot disk cultures at 4, 8 and 12 weeks after inoculation, and at 10, 15, 20 and 25 degrees C. The optimum temperature for reproduction of all populations was 20 degrees C. After 8 weeks at this temperature, nematode numbers increased up to 458-fold, 310-fold and 252-fold for the four populations of P. penetrans, the P. thornei and the P. pseudocoffeae population, respectively.},
  author       = {Mokrini, Fouad and Waeyenberge, Lieven and Viaene, Nicole and Abbad Andaloussi, Fouad and Moens, Maurice},
  issn         = {1388-5545},
  journal      = {NEMATOLOGY},
  keyword      = {RIBOSOMAL DNA,AMMOPHILA-ARENARIA,CV POYO,POPULATIONS,REPRODUCTIVE FITNESS,PHYLOGENETIC-RELATIONSHIPS,RADOPHOLUS-SIMILIS,MOLECULAR CHARACTERIZATION,N. SP NEMATODA,PLANT-PARASITIC NEMATODES,reproductive fitness,rDNA,Pratylenchidae,phylogeny,morphometrics,morphology,D2-D3,molecular},
  language     = {eng},
  pages        = {781--801},
  title        = {Diversity of root-lesion nematodes (Pratylenchus spp.) associated with wheat (Triticum aestivum and T. durum) in Morocco},
  url          = {http://dx.doi.org/10.1163/15685411-00002993},
  volume       = {18},
  year         = {2016},
}

Chicago
Mokrini, Fouad, Lieven Waeyenberge, Nicole Viaene, Fouad Abbad Andaloussi, and Maurice Moens. 2016. “Diversity of Root-lesion Nematodes (Pratylenchus Spp.) Associated with Wheat (Triticum Aestivum and T. Durum) in Morocco.” Nematology 18: 781–801.
APA
Mokrini, F., Waeyenberge, L., Viaene, N., Abbad Andaloussi, F., & Moens, M. (2016). Diversity of root-lesion nematodes (Pratylenchus spp.) associated with wheat (Triticum aestivum and T. durum) in Morocco. NEMATOLOGY, 18, 781–801.
Vancouver
1.
Mokrini F, Waeyenberge L, Viaene N, Abbad Andaloussi F, Moens M. Diversity of root-lesion nematodes (Pratylenchus spp.) associated with wheat (Triticum aestivum and T. durum) in Morocco. NEMATOLOGY. 2016;18:781–801.
MLA
Mokrini, Fouad, Lieven Waeyenberge, Nicole Viaene, et al. “Diversity of Root-lesion Nematodes (Pratylenchus Spp.) Associated with Wheat (Triticum Aestivum and T. Durum) in Morocco.” NEMATOLOGY 18 (2016): 781–801. Print.