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Challenges in the microbial production of flavonoids

Tom Delmulle (UGent) , Sofie De Maeseneire (UGent) and Marjan De Mey (UGent)
(2018) PHYTOCHEMISTRY REVIEWS. 17(2). p.229-247
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Abstract
As flavonoids have beneficial health effects on humans, they are gaining increasing interest from pharmaceutical and health industries. However, current production methods, such as plant extraction and chemical synthesis, are inadequate to meet the demand. Therefore, microbial production might offer a promising alternative. During recent years, microbial strains able to produce flavonoids to a certain extent have been developed. However production titers are limited to the mg l−1 range, hampering the industrial exploitation of these strains. The latter will not be achieved by simply introducing the heterologous pathway in the production host and optimizing the fermentation process, but will depend on the interaction of different aspects of metabolic engineering and process engineering to overcome the current limitations. Next to engineering the production strain to optimize the availability of precursors, the pathway itself also requires intensive engineering. Currently utilized strategies result in a wide variety of different production strains, requiring high-throughput screening methods to identify optimal performing strains. As more and more organisms are being characterized, each with their own specific properties which might be beneficial for the heterologous production of flavonoids, the choice of the production host is another important aspect. Finally, the use of co-cultures might offer an alternative in which different parts of the process are performed by different organisms. This review aims to provide an overview of the research that has been done on these separate aspects. The work presented here could be used as a framework for further research.
Keywords
Synthetic biology, Metabolic engineering, Flavonoids, Microbial cell factories, ENGINEERED ESCHERICHIA-COLI, L-TYROSINE PRODUCTION, CENTRAL METABOLIC PATHWAYS, AMINO-ACID BIOSYNTHESIS, MULTIDRUG EFFLUX PUMP, SACCHAROMYCES-CEREVISIAE, MALONYL-COA, EFFICIENT PRODUCTION, NATURAL-PRODUCTS, BRADYRHIZOBIUM-JAPONICUM

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Citation

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MLA
Delmulle, Tom, Sofie De Maeseneire, and Marjan De Mey. “Challenges in the Microbial Production of Flavonoids.” PHYTOCHEMISTRY REVIEWS 17.2 (2018): 229–247. Print.
APA
Delmulle, T., De Maeseneire, S., & De Mey, M. (2018). Challenges in the microbial production of flavonoids. PHYTOCHEMISTRY REVIEWS, 17(2), 229–247.
Chicago author-date
Delmulle, Tom, Sofie De Maeseneire, and Marjan De Mey. 2018. “Challenges in the Microbial Production of Flavonoids.” Phytochemistry Reviews 17 (2): 229–247.
Chicago author-date (all authors)
Delmulle, Tom, Sofie De Maeseneire, and Marjan De Mey. 2018. “Challenges in the Microbial Production of Flavonoids.” Phytochemistry Reviews 17 (2): 229–247.
Vancouver
1.
Delmulle T, De Maeseneire S, De Mey M. Challenges in the microbial production of flavonoids. PHYTOCHEMISTRY REVIEWS. 2018;17(2):229–47.
IEEE
[1]
T. Delmulle, S. De Maeseneire, and M. De Mey, “Challenges in the microbial production of flavonoids,” PHYTOCHEMISTRY REVIEWS, vol. 17, no. 2, pp. 229–247, 2018.
@article{8110958,
  abstract     = {As flavonoids have beneficial health effects on humans, they are gaining increasing interest from pharmaceutical and health industries. However, current production methods, such as plant extraction and chemical synthesis, are inadequate to meet the demand. Therefore, microbial production might offer a promising alternative. During recent years, microbial strains able to produce flavonoids to a certain extent have been developed. However production titers are limited to the mg l−1 range, hampering the industrial exploitation of these strains. The latter will not be achieved by simply introducing the heterologous pathway in the production host and optimizing the fermentation process, but will depend on the interaction of different aspects of metabolic engineering and process engineering to overcome the current limitations. Next to engineering the production strain to optimize the availability of precursors, the pathway itself also requires intensive engineering. Currently utilized strategies result in a wide variety of different production strains, requiring high-throughput screening methods to identify optimal performing strains. As more and more organisms are being characterized, each with their own specific properties which might be beneficial for the heterologous production of flavonoids, the choice of the production host is another important aspect. Finally, the use of co-cultures might offer an alternative in which different parts of the process are performed by different organisms. This review aims to provide an overview of the research that has been done on these separate aspects. The work presented here could be used as a framework for further research.},
  author       = {Delmulle, Tom and De Maeseneire, Sofie and De Mey, Marjan},
  issn         = {1568-7767},
  journal      = {PHYTOCHEMISTRY REVIEWS},
  keywords     = {Synthetic biology,Metabolic engineering,Flavonoids,Microbial cell factories,ENGINEERED ESCHERICHIA-COLI,L-TYROSINE PRODUCTION,CENTRAL METABOLIC PATHWAYS,AMINO-ACID BIOSYNTHESIS,MULTIDRUG EFFLUX PUMP,SACCHAROMYCES-CEREVISIAE,MALONYL-COA,EFFICIENT PRODUCTION,NATURAL-PRODUCTS,BRADYRHIZOBIUM-JAPONICUM},
  language     = {eng},
  number       = {2},
  pages        = {229--247},
  title        = {Challenges in the microbial production of flavonoids},
  url          = {http://dx.doi.org/10.1007/s11101-017-9515-3},
  volume       = {17},
  year         = {2018},
}

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