Advanced search
2 files | 76.37 MB

Comparison of the adipose and luminal mammary gland compartment as orthotopic inoculation sites in a 4T1-based immunocompetent preclinical model for triple-negative breast cancer

Author
Organization
Abstract
Breast tumorigenesis is classically studied in mice by inoculating tumor cells in the fat pad, the adipose compartment of the mammary gland. Alternatively, the mammary ducts, which constitute the luminal mammary gland compartment, also provide a suitable inoculation site to induce breast cancer in murine models. The microenvironments in these compartments influence tumor cell progression, yet this effect has not been investigated in an immunocompetent context. Here, we compared both mammary gland compartments as distinct inoculation sites, taking into account the immunological aspect by inoculating 4T1 tumor cells in immunocompetent mice. Following tumor cell inoculation in the adipose compartment of non-pretreated/naive, hormonally pretreated/naive and non-pretreated/lactating mice, the primary tumors developed similarly. However, a slower onset of primary tumor growth was found after inoculations in the luminal compartment of non-pretreated/lactating mice. Despite this difference in tumor development rate, metastasis to the liver and lungs was equally observed and was accompanied by lymphatic spreading of tumor cells and progressive splenomegaly with both inoculation types. Chitinase 3-like 1 (CHI3L1) and lipocalin 2 (LCN2) served as innovative biomarkers for disease progression showing increased levels in primary tumors and sera of the non-pretreated/lactating inoculation groups. A slower increase in circulating CHI3L1 but not LCN2 levels, was observed after inoculations in the luminal compartment which corroborated the slower tumor development at this inoculation site. Our results highlight the critical impact of different mammary gland compartments on tumor development in syngeneic murine models and support the use of novel tumor progression biomarkers in an immune-competent environment.
Keywords
adipose and luminal compartment, triple-negative breast cancer, orthotopic inoculation, lipocalin 2, chitinase 3-like 1, 4T1 immunocompetent preclinical model, CARCINOMA IN-SITU, LIPOCALIN 2, TISSUE RESPONSES, LUNG METASTASIS, PROGRESSION, TRANSPLANTATION, 3-LIKE-1, CHI3L1, NGAL, MICE

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 6.19 MB
  • (...).tiff
    • full text
    • |
    • UGent only
    • |
    • image/tiff
    • |
    • 70.18 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Steenbrugge, Jonas, Koen Breyne, Sofie Denies, Melissa Dekimpe, Kristel Demeyere, Olivier De Wever, Peter Vermeulen, Steven Van Laere, Niek Sanders, and Evelyne Meyer. 2016. “Comparison of the Adipose and Luminal Mammary Gland Compartment as Orthotopic Inoculation Sites in a 4T1-based Immunocompetent Preclinical Model for Triple-negative Breast Cancer.” Journal of Mammary Gland Biology and Neoplasia 21 (3-4): 113–122.
APA
Steenbrugge, J., Breyne, K., Denies, S., Dekimpe, M., Demeyere, K., De Wever, O., Vermeulen, P., et al. (2016). Comparison of the adipose and luminal mammary gland compartment as orthotopic inoculation sites in a 4T1-based immunocompetent preclinical model for triple-negative breast cancer. JOURNAL OF MAMMARY GLAND BIOLOGY AND NEOPLASIA, 21(3-4), 113–122.
Vancouver
1.
Steenbrugge J, Breyne K, Denies S, Dekimpe M, Demeyere K, De Wever O, et al. Comparison of the adipose and luminal mammary gland compartment as orthotopic inoculation sites in a 4T1-based immunocompetent preclinical model for triple-negative breast cancer. JOURNAL OF MAMMARY GLAND BIOLOGY AND NEOPLASIA. 2016;21(3-4):113–22.
MLA
Steenbrugge, Jonas et al. “Comparison of the Adipose and Luminal Mammary Gland Compartment as Orthotopic Inoculation Sites in a 4T1-based Immunocompetent Preclinical Model for Triple-negative Breast Cancer.” JOURNAL OF MAMMARY GLAND BIOLOGY AND NEOPLASIA 21.3-4 (2016): 113–122. Print.
@article{8101205,
  abstract     = {Breast tumorigenesis is classically studied in mice by inoculating tumor cells in the fat pad, the adipose compartment of the mammary gland. Alternatively, the mammary ducts, which constitute the luminal mammary gland compartment, also provide a suitable inoculation site to induce breast cancer in murine models. The microenvironments in these compartments influence tumor cell progression, yet this effect has not been investigated in an immunocompetent context. Here, we compared both mammary gland compartments as distinct inoculation sites, taking into account the immunological aspect by inoculating 4T1 tumor cells in immunocompetent mice. Following tumor cell inoculation in the adipose compartment of non-pretreated/naive, hormonally pretreated/naive and non-pretreated/lactating mice, the primary tumors developed similarly. However, a slower onset of primary tumor growth was found after inoculations in the luminal compartment of non-pretreated/lactating mice. Despite this difference in tumor development rate, metastasis to the liver and lungs was equally observed and was accompanied by lymphatic spreading of tumor cells and progressive splenomegaly with both inoculation types. Chitinase 3-like 1 (CHI3L1) and lipocalin 2 (LCN2) served as innovative biomarkers for disease progression showing increased levels in primary tumors and sera of the non-pretreated/lactating inoculation groups. A slower increase in circulating CHI3L1 but not LCN2 levels, was observed after inoculations in the luminal compartment which corroborated the slower tumor development at this inoculation site. Our results highlight the critical impact of different mammary gland compartments on tumor development in syngeneic murine models and support the use of novel tumor progression biomarkers in an immune-competent environment.},
  author       = {Steenbrugge, Jonas and Breyne, Koen and Denies, Sofie and Dekimpe, Melissa and Demeyere, Kristel and De Wever, Olivier and Vermeulen, Peter and Van Laere, Steven and Sanders, Niek and Meyer, Evelyne},
  issn         = {1083-3021},
  journal      = {JOURNAL OF MAMMARY GLAND BIOLOGY AND NEOPLASIA},
  keywords     = {adipose and luminal compartment,triple-negative breast cancer,orthotopic inoculation,lipocalin 2,chitinase 3-like 1,4T1 immunocompetent preclinical model,CARCINOMA IN-SITU,LIPOCALIN 2,TISSUE RESPONSES,LUNG METASTASIS,PROGRESSION,TRANSPLANTATION,3-LIKE-1,CHI3L1,NGAL,MICE},
  language     = {eng},
  number       = {3-4},
  pages        = {113--122},
  title        = {Comparison of the adipose and luminal mammary gland compartment as orthotopic inoculation sites in a 4T1-based immunocompetent preclinical model for triple-negative breast cancer},
  url          = {http://dx.doi.org/10.1007/s10911-016-9362-7},
  volume       = {21},
  year         = {2016},
}

Altmetric
View in Altmetric
Web of Science
Times cited: