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Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.)

(2008) MOLECULAR BREEDING. 21(4). p.533-548
Author
Organization
Abstract
An expressed sequence tag (EST) library of the key grassland species perennial ryegrass (Lolium perenne L.) has been exploited as a resource for microsatellite marker development. Out of 955 simple sequence repeat (SSR) containing ESTs, 744 were used for primer design. Primer amplification was tested in eight genotypes of L. perenne and L. multiflorum representing (grand-) parents of four mapping populations and resulted in 464 successfully amplified EST-SSRs. Three hundred and six primer pairs successfully amplified products in the mapping population VrnA derived from two of the eight genotypes included in the original screening and revealed SSR polymorphisms for 143 ESTs. Here, we report on 464 EST-derived SSR primer sequences of perennial ryegrass established in laboratory assays, providing a dedicated tool for marker assisted breeding and comparative mapping within and among forage and turf grasses.
Keywords
ryegrass (Lolium spp.), simple sequence repeat (SSR), expressed sequence tag (EST), comparative mapping, REPEAT SSR MARKERS, MULTIFLORUM LAM., LINKAGE MAP, PLANTS, TRANSFERABILITY, CONSTRUCTION, REVEALS, QTL

Citation

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MLA
Studer, Bruno et al. “Expressed Sequence Tag-derived Microsatellite Markers of Perennial Ryegrass (Lolium Perenne L.).” MOLECULAR BREEDING 21.4 (2008): 533–548. Print.
APA
Studer, B., Asp, T., Frei, U., Hentrup, S., Meally, H., Guillard, A., Barth, S., et al. (2008). Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.). MOLECULAR BREEDING, 21(4), 533–548.
Chicago author-date
Studer, Bruno, Torben Asp, Ursula Frei, Stephan Hentrup, Helena Meally, Aurelie Guillard, Susanne Barth, et al. 2008. “Expressed Sequence Tag-derived Microsatellite Markers of Perennial Ryegrass (Lolium Perenne L.).” Molecular Breeding 21 (4): 533–548.
Chicago author-date (all authors)
Studer, Bruno, Torben Asp, Ursula Frei, Stephan Hentrup, Helena Meally, Aurelie Guillard, Susanne Barth, Hilde Muylle, Isabel Roldàn-Ruiz, Philippe Barre, Carole Koning-Boucoiran, Gerda Uenk-Stunnenberg, Oene Dolstra, Leif Skot, Kirsten P Skot, Lesley B Turner, Mervyn O Humphreys, Roland Kolliker, Niels Roulund, Klaus K Nielsen, and Thomas Lubberstedt. 2008. “Expressed Sequence Tag-derived Microsatellite Markers of Perennial Ryegrass (Lolium Perenne L.).” Molecular Breeding 21 (4): 533–548.
Vancouver
1.
Studer B, Asp T, Frei U, Hentrup S, Meally H, Guillard A, et al. Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.). MOLECULAR BREEDING. 2008;21(4):533–48.
IEEE
[1]
B. Studer et al., “Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.),” MOLECULAR BREEDING, vol. 21, no. 4, pp. 533–548, 2008.
@article{8096415,
  abstract     = {An expressed sequence tag (EST) library of the key grassland species perennial ryegrass (Lolium perenne L.) has been exploited as a resource for microsatellite marker development. Out of 955 simple sequence repeat (SSR) containing ESTs, 744 were used for primer design. Primer amplification was tested in eight genotypes of L. perenne and L. multiflorum representing (grand-) parents of four mapping populations and resulted in 464 successfully amplified EST-SSRs. Three hundred and six primer pairs successfully amplified products in the mapping population VrnA derived from two of the eight genotypes included in the original screening and revealed SSR polymorphisms for 143 ESTs. Here, we report on 464 EST-derived SSR primer sequences of perennial ryegrass established in laboratory assays, providing a dedicated tool for marker assisted breeding and comparative mapping within and among forage and turf grasses.},
  author       = {Studer, Bruno and Asp, Torben and Frei, Ursula and Hentrup, Stephan and Meally, Helena and Guillard, Aurelie and Barth, Susanne and Muylle, Hilde and Roldàn-Ruiz, Isabel and Barre, Philippe and Koning-Boucoiran, Carole and Uenk-Stunnenberg, Gerda and Dolstra, Oene and Skot, Leif and Skot, Kirsten P and Turner, Lesley B and Humphreys, Mervyn O and Kolliker, Roland and Roulund, Niels and Nielsen, Klaus K and Lubberstedt, Thomas},
  issn         = {1380-3743},
  journal      = {MOLECULAR BREEDING},
  keywords     = {ryegrass (Lolium spp.),simple sequence repeat (SSR),expressed sequence tag (EST),comparative mapping,REPEAT SSR MARKERS,MULTIFLORUM LAM.,LINKAGE MAP,PLANTS,TRANSFERABILITY,CONSTRUCTION,REVEALS,QTL},
  language     = {eng},
  number       = {4},
  pages        = {533--548},
  title        = {Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.)},
  url          = {http://dx.doi.org/10.1007/s11032-007-9148-0},
  volume       = {21},
  year         = {2008},
}

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