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Sucrose preculture to simplify cryopreservation of banana meristem cultures

(2002) CRYOLETTERS. 23(6). p.375-384
Author
Organization
Abstract
A simple cryopreservation method is. described for proliferating meristem cultures of banana (Musa spp.). It relies on a 2-week preculture on media containing 0.4 M sucrose followed by rapid cooling in liquid nitrogen. Different preculture media were screened for efficient protection of banana meristems during cryopreservation. Sucrose can be replaced by both fructose and glucose without significantly affecting post-thaw survival. A high BA concentration (100 muM) in the preculture medium results in less material available for cryopreservation, but does not affect cryoprotection. Culture in liquid media significantly improved post-thaw regeneration. The optimized cryopreservation protocol was applied on 36 banana accessions belonging to 8 different genomic groups. It is shown that post-thaw regeneration frequencies (ranging between 0 and 66%) are highly dependent on the genomic constitution of the banana cultivar.
Keywords
glucose, meristem culture, sugar, sucrose, fructose, cryoprotection, benzyladenine, cryopreservation, banana, Musa spp., ASPARAGUS-OFFICINALIS L, CARROT SOMATIC EMBRYOS, SHOOT TIPS, ENCAPSULATION-VITRIFICATION, AXILLARY BUDS, TERM STORAGE, MUSA, TOLERANCE, ACQUISITION, SUGAR

Citation

Please use this url to cite or link to this publication:

MLA
Panis, Bart et al. “Sucrose Preculture to Simplify Cryopreservation of Banana Meristem Cultures.” CRYOLETTERS 23.6 (2002): 375–384. Print.
APA
Panis, B., Strosse, H., Van Den Hende, S., & Swennen, R. (2002). Sucrose preculture to simplify cryopreservation of banana meristem cultures. CRYOLETTERS, 23(6), 375–384.
Chicago author-date
Panis, Bart, Hannelore Strosse, Sofie Van Den Hende, and Rony Swennen. 2002. “Sucrose Preculture to Simplify Cryopreservation of Banana Meristem Cultures.” Cryoletters 23 (6): 375–384.
Chicago author-date (all authors)
Panis, Bart, Hannelore Strosse, Sofie Van Den Hende, and Rony Swennen. 2002. “Sucrose Preculture to Simplify Cryopreservation of Banana Meristem Cultures.” Cryoletters 23 (6): 375–384.
Vancouver
1.
Panis B, Strosse H, Van Den Hende S, Swennen R. Sucrose preculture to simplify cryopreservation of banana meristem cultures. CRYOLETTERS. 2002;23(6):375–84.
IEEE
[1]
B. Panis, H. Strosse, S. Van Den Hende, and R. Swennen, “Sucrose preculture to simplify cryopreservation of banana meristem cultures,” CRYOLETTERS, vol. 23, no. 6, pp. 375–384, 2002.
@article{808725,
  abstract     = {A simple cryopreservation method is. described for proliferating meristem cultures of banana (Musa spp.). It relies on a 2-week preculture on media containing 0.4 M sucrose followed by rapid cooling in liquid nitrogen. Different preculture media were screened for efficient protection of banana meristems during cryopreservation. Sucrose can be replaced by both fructose and glucose without significantly affecting post-thaw survival. A high BA concentration (100 muM) in the preculture medium results in less material available for cryopreservation, but does not affect cryoprotection. Culture in liquid media significantly improved post-thaw regeneration. The optimized cryopreservation protocol was applied on 36 banana accessions belonging to 8 different genomic groups. It is shown that post-thaw regeneration frequencies (ranging between 0 and 66%) are highly dependent on the genomic constitution of the banana cultivar.},
  author       = {Panis, Bart and Strosse, Hannelore and Van Den Hende, Sofie and Swennen, Rony},
  issn         = {0143-2044},
  journal      = {CRYOLETTERS},
  keywords     = {glucose,meristem culture,sugar,sucrose,fructose,cryoprotection,benzyladenine,cryopreservation,banana,Musa spp.,ASPARAGUS-OFFICINALIS L,CARROT SOMATIC EMBRYOS,SHOOT TIPS,ENCAPSULATION-VITRIFICATION,AXILLARY BUDS,TERM STORAGE,MUSA,TOLERANCE,ACQUISITION,SUGAR},
  language     = {eng},
  number       = {6},
  pages        = {375--384},
  title        = {Sucrose preculture to simplify cryopreservation of banana meristem cultures},
  volume       = {23},
  year         = {2002},
}

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