Ghent University Academic Bibliography

Advanced

Synthetic biology of modular proteins

Veerle Maervoet and Yves Briers UGent (2017) BIOENGINEERED. 8(3). p.196-202
abstract
The evolution of natural modular proteins and domain swapping by protein engineers have shown the disruptive potential of non-homologous recombination to create proteins with novel functions or traits. Bacteriophage endolysins, cellulosomes and polyketide synthases are three examples of natural modular proteins with each module having a dedicated function. These modular architectures have been created by extensive duplication, shuffling of domains and insertion/deletion of new domains. Protein engineers mimic these natural processes in vitro to create chimeras with altered properties or novel functions by swapping modules between different parental genes. Most domain swapping efforts are realized with traditional restriction and ligation techniques, which become particularly restrictive when either a large number of variants, or variants of proteins with multiple domains have to be constructed. Recent advances in homology-independent shuffling techniques increasingly address this need, but to realize the full potential of the synthetic biology of modular proteins a complete homology-independent method for both rational and random shuffling of modules from an unlimited number of parental genes is still needed.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
polyketide synthase, horizontal transfer, protein engineering, endolysin, designer cellulosome, Modular protein, domain swapping, POLYKETIDE SYNTHASES, CELLULOSE DEGRADATION, BIOSYNTHESIS, ENDOLYSINS, EVOLUTION, HOMOLOGY, LISTERIA, LIBRARY, ENZYME, CONSTRUCTION
journal title
BIOENGINEERED
Bioengineered
volume
8
issue
3
pages
196 - 202
Web of Science type
Article
Web of Science id
000402878800009
ISSN
2165-5979
DOI
10.1080/21655979.2016.1222993
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8086542
handle
http://hdl.handle.net/1854/LU-8086542
date created
2016-09-23 20:19:53
date last changed
2017-06-21 09:19:38
@article{8086542,
  abstract     = {The evolution of natural modular proteins and domain swapping by protein engineers have shown the disruptive potential of non-homologous recombination to create proteins with novel functions or traits. Bacteriophage endolysins, cellulosomes and polyketide synthases are three examples of natural modular proteins with each module having a dedicated function. These modular architectures have been created by extensive duplication, shuffling of domains and insertion/deletion of new domains. Protein engineers mimic these natural processes in vitro to create chimeras with altered properties or novel functions by swapping modules between different parental genes. Most domain swapping efforts are realized with traditional restriction and ligation techniques, which become particularly restrictive when either a large number of variants, or variants of proteins with multiple domains have to be constructed. Recent advances in homology-independent shuffling techniques increasingly address this need, but to realize the full potential of the synthetic biology of modular proteins a complete homology-independent method for both rational and random shuffling of modules from an unlimited number of parental genes is still needed.},
  author       = {Maervoet, Veerle and Briers, Yves},
  issn         = {2165-5979},
  journal      = {BIOENGINEERED},
  keyword      = {polyketide synthase,horizontal transfer,protein engineering,endolysin,designer cellulosome,Modular protein,domain swapping,POLYKETIDE SYNTHASES,CELLULOSE DEGRADATION,BIOSYNTHESIS,ENDOLYSINS,EVOLUTION,HOMOLOGY,LISTERIA,LIBRARY,ENZYME,CONSTRUCTION},
  language     = {eng},
  number       = {3},
  pages        = {196--202},
  title        = {Synthetic biology of modular proteins},
  url          = {http://dx.doi.org/10.1080/21655979.2016.1222993},
  volume       = {8},
  year         = {2017},
}

Chicago
Maervoet, Veerle, and Yves Briers. 2017. “Synthetic Biology of Modular Proteins.” Bioengineered 8 (3): 196–202.
APA
Maervoet, V., & Briers, Y. (2017). Synthetic biology of modular proteins. BIOENGINEERED, 8(3), 196–202.
Vancouver
1.
Maervoet V, Briers Y. Synthetic biology of modular proteins. BIOENGINEERED. 2017;8(3):196–202.
MLA
Maervoet, Veerle, and Yves Briers. “Synthetic Biology of Modular Proteins.” BIOENGINEERED 8.3 (2017): 196–202. Print.