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Species specificity of bacteria associated to the brown seaweeds Lobophora (Dictyotales, Phaeophyceae) and their potential for induction of rapid coral bleaching in Acropora muricata

Christophe Vieira UGent, Aschwin H Engelen, Linda Guentas, Tânia Aires, Fanny Houlbreque, Julie Gaubert, Ester A Serrão, Olivier De Clerck UGent and Claude E Payri (2016) FRONTIERS IN MICROBIOLOGY. 7.
abstract
While reef degradation is occurring worldwide, it is not uncommon to see phase shifts from coral to macroalgal dominated reefs. Numerous studies have addressed the mechanisms by which macroalgae may outcompete corals and a few recent studies highlighted the putative role of bacteria at the interface between macroalgae and corals. Some studies suggest that macroalgae may act as vectors and/or foster proliferation of microorganisms pathogenic for corals. Using a combination of high throughput sequencing, bacterial culturing, and in situ bioassays we question if the adversity of macroalgal associated bacteria to corals is mediated by specific bacterial taxa. Using Illumina sequencing, we characterized and compared the bacterial community from two Lobophora (Dictyotales, Phaeophyceae) species. The two species presented distinctive bacterial communities. Both species shared approximately half of their OTUs, mainly the most abundant bacteria. Species-specific OTUs belong to Planctomycetes, Proteobacteria, and Bacteroidetes. In total, 16 culturable bacterial strain were isolated and identified from the Lobophora surface, consisting of 10 genera (from nine families, four classes, and three phyla), some of which are not known as, but are related to pathogens involved in coral diseases, and others are naturally associated to corals. When patches of marine agar with 24 h cultures of each of these bacteria were placed in direct contact with the branches of the scleractinian coral Acropora muricata, they caused severe bleaching after 24 h exposure. Results suggest that regardless of taxonomic affinities, increase in density of these bacteria can be adverse to corals. Nevertheless, the microbial community associated to macroalgal surface may not represent a threat to corals, because the specific bacterial screening and control exerted by the alga preventing specific bacterial proliferation.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
macroalgal-coral interaction, macroalgal bacterial assemblage, Lobophora, in situ bioassay, Illumina sequencing, coral bleaching, COMMUNITIES, HERBIVORY, VARIEGATA, DIVERSITY, COMPETITION, REEF, ALGAE, HARD CORAL, MONTASTRAEA-ANNULARIS, PHYLOGENETIC CHARACTERIZATION, macroalgal culturable epibacteria
journal title
FRONTIERS IN MICROBIOLOGY
Front. Microbiol.
volume
7
article number
316
pages
13 pages
Web of Science type
Article
Web of Science id
000372491100001
JCR category
MICROBIOLOGY
JCR impact factor
4.076 (2016)
JCR rank
25/124 (2016)
JCR quartile
1 (2016)
ISSN
1664-302X
DOI
10.3389/fmicb.2016.00316
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
8085356
handle
http://hdl.handle.net/1854/LU-8085356
date created
2016-09-22 16:29:19
date last changed
2017-02-15 15:04:11
@article{8085356,
  abstract     = {While reef degradation is occurring worldwide, it is not uncommon to see phase shifts from coral to macroalgal dominated reefs. Numerous studies have addressed the mechanisms by which macroalgae may outcompete corals and a few recent studies highlighted the putative role of bacteria at the interface between macroalgae and corals. Some studies suggest that macroalgae may act as vectors and/or foster proliferation of microorganisms pathogenic for corals. Using a combination of high throughput sequencing, bacterial culturing, and in situ bioassays we question if the adversity of macroalgal associated bacteria to corals is mediated by specific bacterial taxa. Using Illumina sequencing, we characterized and compared the bacterial community from two Lobophora (Dictyotales, Phaeophyceae) species. The two species presented distinctive bacterial communities. Both species shared approximately half of their OTUs, mainly the most abundant bacteria. Species-specific OTUs belong to Planctomycetes, Proteobacteria, and Bacteroidetes. In total, 16 culturable bacterial strain were isolated and identified from the Lobophora surface, consisting of 10 genera (from nine families, four classes, and three phyla), some of which are not known as, but are related to pathogens involved in coral diseases, and others are naturally associated to corals. When patches of marine agar with 24 h cultures of each of these bacteria were placed in direct contact with the branches of the scleractinian coral Acropora muricata, they caused severe bleaching after 24 h exposure. Results suggest that regardless of taxonomic affinities, increase in density of these bacteria can be adverse to corals. Nevertheless, the microbial community associated to macroalgal surface may not represent a threat to corals, because the specific bacterial screening and control exerted by the alga preventing specific bacterial proliferation.},
  articleno    = {316},
  author       = {Vieira, Christophe and Engelen, Aschwin H and Guentas, Linda and Aires, T{\^a}nia and Houlbreque, Fanny and Gaubert, Julie and Serr{\~a}o, Ester A and De Clerck, Olivier and Payri, Claude E},
  issn         = {1664-302X},
  journal      = {FRONTIERS IN MICROBIOLOGY},
  keyword      = {macroalgal-coral interaction,macroalgal bacterial assemblage,Lobophora,in situ bioassay,Illumina sequencing,coral bleaching,COMMUNITIES,HERBIVORY,VARIEGATA,DIVERSITY,COMPETITION,REEF,ALGAE,HARD CORAL,MONTASTRAEA-ANNULARIS,PHYLOGENETIC CHARACTERIZATION,macroalgal culturable epibacteria},
  language     = {eng},
  pages        = {13},
  title        = {Species specificity of bacteria associated to the brown seaweeds Lobophora (Dictyotales, Phaeophyceae) and their potential for induction of rapid coral bleaching in Acropora muricata},
  url          = {http://dx.doi.org/10.3389/fmicb.2016.00316},
  volume       = {7},
  year         = {2016},
}

Chicago
Vieira, Christophe, Aschwin H Engelen, Linda Guentas, Tânia Aires, Fanny Houlbreque, Julie Gaubert, Ester A Serrão, Olivier De Clerck, and Claude E Payri. 2016. “Species Specificity of Bacteria Associated to the Brown Seaweeds Lobophora (Dictyotales, Phaeophyceae) and Their Potential for Induction of Rapid Coral Bleaching in Acropora Muricata.” Frontiers in Microbiology 7.
APA
Vieira, C., Engelen, A. H., Guentas, L., Aires, T., Houlbreque, F., Gaubert, J., Serrão, E. A., et al. (2016). Species specificity of bacteria associated to the brown seaweeds Lobophora (Dictyotales, Phaeophyceae) and their potential for induction of rapid coral bleaching in Acropora muricata. FRONTIERS IN MICROBIOLOGY, 7.
Vancouver
1.
Vieira C, Engelen AH, Guentas L, Aires T, Houlbreque F, Gaubert J, et al. Species specificity of bacteria associated to the brown seaweeds Lobophora (Dictyotales, Phaeophyceae) and their potential for induction of rapid coral bleaching in Acropora muricata. FRONTIERS IN MICROBIOLOGY. 2016;7.
MLA
Vieira, Christophe, Aschwin H Engelen, Linda Guentas, et al. “Species Specificity of Bacteria Associated to the Brown Seaweeds Lobophora (Dictyotales, Phaeophyceae) and Their Potential for Induction of Rapid Coral Bleaching in Acropora Muricata.” FRONTIERS IN MICROBIOLOGY 7 (2016): n. pag. Print.