Advanced search
1 file | 248.97 KB Add to list

Determination of iohexol and iothalamate in serum and urine by capillary electrophoresis

Sofie Van Houcke (UGent) , LIESBETH SEAUX (UGent) , Etienne Cavalier, Marijn Speeckaert (UGent) , Els Dumoulin (UGent) , Elke Lecocq (UGent) and Joris Delanghe (UGent)
(2016) ELECTROPHORESIS. 37(17-18). p.2363-2367
Author
Organization
Abstract
Estimation of glomerular filtration rate (eGFR) is essential to assess kidney function. Iodine-containing contrast agents detection by HPLC has been proposed as a safe alternative for inulin or radioactive compounds. However, HPLC is a time-consuming and labor-intensive method. The aim of this study was to develop an assay for iohexol and iothalamate using capillary electrophoresis. Iohexol and iothalamate were directly analyzed by CE in serum and urine, using photometric detection (246 nm). Serum peak height was proportional to iohexol and iothalamate concentrations. Detection limits for iohexol and iothalamate were 10 and 5 mg/L. Limits of quantification were 13.0 and 15.0 mg/L. Within-run CVs were 4.9 and 6.5%; between-run CVs 3.1-9.9% and 3.8-13.7%. A good correlation was observed between CE and HPLC: y = 1.1703x + 5.017 (iohexol) and y = 0.7807x + 11.01 (iothalamate; (y = concentration obtained by CE [mg/L], x = concentration obtained by HPLC [mg/L]). In addition, CE allowed to determine urinary iohexol concentration. Although the detection limit for CE was higher than for HPLC, CE can still be used for eGFR determination. Advantages of this high-throughput method are the absence of sample pretreatment and a minimal sample volume requirement.
Keywords
Capillary electrophoresis, Iodine-containing contrast media, Glomerular filtration rate, GLOMERULAR-FILTRATION-RATE, CONTRAST AGENT, CHROMATOGRAPHY, QUANTIFICATION, CLEARANCE, GFR

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 248.97 KB

Citation

Please use this url to cite or link to this publication:

MLA
Van Houcke, Sofie, et al. “Determination of Iohexol and Iothalamate in Serum and Urine by Capillary Electrophoresis.” ELECTROPHORESIS, vol. 37, no. 17–18, 2016, pp. 2363–67.
APA
Van Houcke, S., SEAUX, L., Cavalier, E., Speeckaert, M., Dumoulin, E., Lecocq, E., & Delanghe, J. (2016). Determination of iohexol and iothalamate in serum and urine by capillary electrophoresis. ELECTROPHORESIS, 37(17–18), 2363–2367.
Chicago author-date
Van Houcke, Sofie, LIESBETH SEAUX, Etienne Cavalier, Marijn Speeckaert, Els Dumoulin, Elke Lecocq, and Joris Delanghe. 2016. “Determination of Iohexol and Iothalamate in Serum and Urine by Capillary Electrophoresis.” ELECTROPHORESIS 37 (17–18): 2363–67.
Chicago author-date (all authors)
Van Houcke, Sofie, LIESBETH SEAUX, Etienne Cavalier, Marijn Speeckaert, Els Dumoulin, Elke Lecocq, and Joris Delanghe. 2016. “Determination of Iohexol and Iothalamate in Serum and Urine by Capillary Electrophoresis.” ELECTROPHORESIS 37 (17–18): 2363–2367.
Vancouver
1.
Van Houcke S, SEAUX L, Cavalier E, Speeckaert M, Dumoulin E, Lecocq E, et al. Determination of iohexol and iothalamate in serum and urine by capillary electrophoresis. ELECTROPHORESIS. 2016;37(17–18):2363–7.
IEEE
[1]
S. Van Houcke et al., “Determination of iohexol and iothalamate in serum and urine by capillary electrophoresis,” ELECTROPHORESIS, vol. 37, no. 17–18, pp. 2363–2367, 2016.
@article{8084401,
  abstract     = {{Estimation of glomerular filtration rate (eGFR) is essential to assess kidney function. Iodine-containing contrast agents detection by HPLC has been proposed as a safe alternative for inulin or radioactive compounds. However, HPLC is a time-consuming and labor-intensive method. The aim of this study was to develop an assay for iohexol and iothalamate using capillary electrophoresis. Iohexol and iothalamate were directly analyzed by CE in serum and urine, using photometric detection (246 nm). Serum peak height was proportional to iohexol and iothalamate concentrations. Detection limits for iohexol and iothalamate were 10 and 5 mg/L. Limits of quantification were 13.0 and 15.0 mg/L. Within-run CVs were 4.9 and 6.5%; between-run CVs 3.1-9.9% and 3.8-13.7%. A good correlation was observed between CE and HPLC: y = 1.1703x + 5.017 (iohexol) and y = 0.7807x + 11.01 (iothalamate; (y = concentration obtained by CE [mg/L], x = concentration obtained by HPLC [mg/L]). In addition, CE allowed to determine urinary iohexol concentration. Although the detection limit for CE was higher than for HPLC, CE can still be used for eGFR determination. Advantages of this high-throughput method are the absence of sample pretreatment and a minimal sample volume requirement.}},
  author       = {{Van Houcke, Sofie and SEAUX, LIESBETH and Cavalier, Etienne and Speeckaert, Marijn and Dumoulin, Els and Lecocq, Elke and Delanghe, Joris}},
  issn         = {{0173-0835}},
  journal      = {{ELECTROPHORESIS}},
  keywords     = {{Capillary electrophoresis,Iodine-containing contrast media,Glomerular filtration rate,GLOMERULAR-FILTRATION-RATE,CONTRAST AGENT,CHROMATOGRAPHY,QUANTIFICATION,CLEARANCE,GFR}},
  language     = {{eng}},
  number       = {{17-18}},
  pages        = {{2363--2367}},
  title        = {{Determination of iohexol and iothalamate in serum and urine by capillary electrophoresis}},
  url          = {{http://dx.doi.org/10.1002/elps.201600084}},
  volume       = {{37}},
  year         = {{2016}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: