Effects of electromagnetic stimulation on osteogenic differentiation of human mesenchymal stromal cells seeded onto gelatin cryogel
- Author
- Enrica Saino, Lorenzo Fassina, Sandra Van Vlierberghe (UGent) , Maria Avanzini, Peter Dubruel (UGent) , Giovanni Magenes, Livia Visai and Francesco Benazzo
- Organization
- Abstract
- Bone tissue engineering typically uses biomaterial scaffolds, osteoblasts or cells that can become osteoblasts, and biophysical stimulations to promote cell attachment and differentiation. In this study, we investigated the effects of an electromagnetic wave on mesenchymal stromal cells isolated from the bone marrow and seeded upon gelatin cryogel disks. In comparison with control conditions without electromagnetic stimulus, the electromagnetic treatment (magnetic field, 2 mT; frequency, 75 Hz) increased the cell proliferation and differentiation and enhanced the biomaterial surface coating with bone extracellular matrix proteins. Using this tissue-engineering approach, the gelatin biomaterial, coated with differentiated cells and their extracellular matrix proteins, may be used in clinical applications as an implant for bone defect repair.
- Keywords
- mesenchymal stromal cells, bovine gelatin cryogel, electromagnetic bioreactor, bone matrix proteins
Downloads
-
International Journal of Immunopathology and Pharmacology 1.pdf
- full text (Published version)
- |
- open access
- |
- |
- 1.63 MB
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8082280
- MLA
- Saino, Enrica, et al. “Effects of Electromagnetic Stimulation on Osteogenic Differentiation of Human Mesenchymal Stromal Cells Seeded onto Gelatin Cryogel.” INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY, vol. 24, no. 1, Supplement 2, 2011, pp. 1–6.
- APA
- Saino, E., Fassina, L., Van Vlierberghe, S., Avanzini, M., Dubruel, P., Magenes, G., … Benazzo, F. (2011). Effects of electromagnetic stimulation on osteogenic differentiation of human mesenchymal stromal cells seeded onto gelatin cryogel. INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY, 24(1, Supplement 2), 1–6.
- Chicago author-date
- Saino, Enrica, Lorenzo Fassina, Sandra Van Vlierberghe, Maria Avanzini, Peter Dubruel, Giovanni Magenes, Livia Visai, and Francesco Benazzo. 2011. “Effects of Electromagnetic Stimulation on Osteogenic Differentiation of Human Mesenchymal Stromal Cells Seeded onto Gelatin Cryogel.” INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY 24 (1, Supplement 2): 1–6.
- Chicago author-date (all authors)
- Saino, Enrica, Lorenzo Fassina, Sandra Van Vlierberghe, Maria Avanzini, Peter Dubruel, Giovanni Magenes, Livia Visai, and Francesco Benazzo. 2011. “Effects of Electromagnetic Stimulation on Osteogenic Differentiation of Human Mesenchymal Stromal Cells Seeded onto Gelatin Cryogel.” INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY 24 (1, Supplement 2): 1–6.
- Vancouver
- 1.Saino E, Fassina L, Van Vlierberghe S, Avanzini M, Dubruel P, Magenes G, et al. Effects of electromagnetic stimulation on osteogenic differentiation of human mesenchymal stromal cells seeded onto gelatin cryogel. INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY. 2011;24(1, Supplement 2):1–6.
- IEEE
- [1]E. Saino et al., “Effects of electromagnetic stimulation on osteogenic differentiation of human mesenchymal stromal cells seeded onto gelatin cryogel,” INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY, vol. 24, no. 1, Supplement 2, pp. 1–6, 2011.
@article{8082280,
abstract = {{Bone tissue engineering typically uses biomaterial scaffolds, osteoblasts or cells that can become osteoblasts, and biophysical stimulations to promote cell attachment and differentiation. In this study, we investigated the effects of an electromagnetic wave on mesenchymal stromal cells isolated from the bone marrow and seeded upon gelatin cryogel disks. In comparison with control conditions without electromagnetic stimulus, the electromagnetic treatment (magnetic field, 2 mT; frequency, 75 Hz) increased the cell proliferation and differentiation and enhanced the biomaterial surface coating with bone extracellular matrix proteins. Using this tissue-engineering approach, the gelatin biomaterial, coated with differentiated cells and their extracellular matrix proteins, may be used in clinical applications as an implant for bone defect repair.}},
author = {{Saino, Enrica and Fassina, Lorenzo and Van Vlierberghe, Sandra and Avanzini, Maria and Dubruel, Peter and Magenes, Giovanni and Visai, Livia and Benazzo, Francesco}},
issn = {{0394-6320}},
journal = {{INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY}},
keywords = {{mesenchymal stromal cells,bovine gelatin cryogel,electromagnetic bioreactor,bone matrix proteins}},
language = {{eng}},
number = {{1, Supplement 2}},
pages = {{1--6}},
title = {{Effects of electromagnetic stimulation on osteogenic differentiation of human mesenchymal stromal cells seeded onto gelatin cryogel}},
volume = {{24}},
year = {{2011}},
}