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Optical manipulation of single magnetic beads in a microwell Array on a digital microfluidic chip

Deborah Decrop, Toon Brans UGent, Pieter Gijsenbergh, Jiadi Lu, Dragana Spasic, Tadej Kokalj, Filip Beunis UGent, Peter Goos, Robert Puers and Jeroen Lammertyn (2016) ANALYTICAL CHEMISTRY . 88(17). p.8596-8603
abstract
The detection of single molecules in magnetic microbead microwell array formats revolutionized the development of digital bioassays. However, retrieval of individual magnetic beads from these arrays has not been realized until now despite having great potential for studying captured targets at the individual level. In this paper, optical tweezers were implemented on a digital microfluidic platform for accurate manipulation of single magnetic beads seeded in a microwell array. Successful optical trapping of magnetic beads was found to be dependent on Brownian motion of the beads, suggesting a 99% chance of trapping a vibrating bead. A tailor-made experimental design was used to screen the effect of bead type, ionic buffer strength, surfactant type, and concentration on the Brownian activity of beads in microwells. With the optimal conditions, the manipulation of magnetic beads was demonstrated by their trapping, retrieving, transporting, and repositioning to a desired microwell on the array. The presented platform combines the strengths of digital microfluidics, digital bioassays, and optical tweezers, resulting in a powerful dynamic microwell array system for single molecule and single cell studies.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
journal title
ANALYTICAL CHEMISTRY
volume
88
issue
17
pages
8 pages
publisher
American Chemical Society
Web of Science type
Article
Web of Science id
000382805900037
ISSN
0003-2700
DOI
10.1021/acs.analchem.6b01734
language
English
UGent publication?
yes
classification
A1
id
8079879
handle
http://hdl.handle.net/1854/LU-8079879
date created
2016-09-19 11:41:58
date last changed
2017-08-25 08:44:02
@article{8079879,
  abstract     = {The detection of single molecules in magnetic microbead microwell array formats revolutionized the development of digital bioassays. However, retrieval of individual magnetic beads from these arrays has not been realized until now despite having great potential for studying captured targets at the individual level. In this paper, optical tweezers were implemented on a digital microfluidic platform for accurate manipulation of single magnetic beads seeded in a microwell array. Successful optical trapping of magnetic beads was found to be dependent on Brownian motion of the beads, suggesting a 99\% chance of trapping a vibrating bead. A tailor-made experimental design was used to screen the effect of bead type, ionic buffer strength, surfactant type, and concentration on the Brownian activity of beads in microwells. With the optimal conditions, the manipulation of magnetic beads was demonstrated by their trapping, retrieving, transporting, and repositioning to a desired microwell on the array. The presented platform combines the strengths of digital microfluidics, digital bioassays, and optical tweezers, resulting in a powerful dynamic microwell array system for single molecule and single cell studies.},
  author       = {Decrop, Deborah and Brans, Toon and Gijsenbergh, Pieter  and Lu, Jiadi and Spasic, Dragana and Kokalj, Tadej and Beunis, Filip and Goos, Peter and Puers, Robert and Lammertyn, Jeroen},
  issn         = {0003-2700 },
  journal      = {ANALYTICAL CHEMISTRY },
  language     = {eng},
  number       = {17},
  pages        = {8596--8603},
  publisher    = {American Chemical Society},
  title        = {Optical manipulation of single magnetic beads in a microwell Array on a digital microfluidic chip},
  url          = {http://dx.doi.org/10.1021/acs.analchem.6b01734},
  volume       = {88},
  year         = {2016},
}

Chicago
Decrop, Deborah, Toon Brans, Pieter Gijsenbergh, Jiadi Lu, Dragana Spasic, Tadej Kokalj, Filip Beunis, Peter Goos, Robert Puers, and Jeroen Lammertyn. 2016. “Optical Manipulation of Single Magnetic Beads in a Microwell Array on a Digital Microfluidic Chip.” Analytical Chemistry 88 (17): 8596–8603.
APA
Decrop, D., Brans, T., Gijsenbergh, P., Lu, J., Spasic, D., Kokalj, T., Beunis, F., et al. (2016). Optical manipulation of single magnetic beads in a microwell Array on a digital microfluidic chip. ANALYTICAL CHEMISTRY , 88(17), 8596–8603.
Vancouver
1.
Decrop D, Brans T, Gijsenbergh P, Lu J, Spasic D, Kokalj T, et al. Optical manipulation of single magnetic beads in a microwell Array on a digital microfluidic chip. ANALYTICAL CHEMISTRY . American Chemical Society; 2016;88(17):8596–603.
MLA
Decrop, Deborah, Toon Brans, Pieter Gijsenbergh, et al. “Optical Manipulation of Single Magnetic Beads in a Microwell Array on a Digital Microfluidic Chip.” ANALYTICAL CHEMISTRY 88.17 (2016): 8596–8603. Print.