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Enzymatic production of alpha-d-galactose 1-phosphate by lactose phosphorolysis

Manu De Groeve (UGent) , Veerle Depreitere (UGent) , Tom Desmet (UGent) and Wim Soetaert (UGent)
(2009) BIOTECHNOLOGY LETTERS. 31(12). p.1873-1877
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Organization
Abstract
alpha-d-Galactose 1-phosphate (alpha Gal1P) is an important building block for the synthesis of nucleotide sugars that are substrates for glycosyltransferases. We have previously reported the creation of novel lactose phosphorylase enzymes that are useful for the synthesis of alpha Gal1P from the cheap and abundant lactose. Here we describe the application of such a lactose phosphorylase in a production system using permeabilized Escherichia coli cells. After purification of the product from the reaction mixture by anion-exchange chromatography and ethanol precipitation, 9.5 grams of highly pure alpha Gal1P were obtained from a 1 l reaction volume.
Keywords
Galactose 1-phosphate, Bioconversion, Lactose phosphorylase, Phosphorolysis, Purification, Synthesis, CELLOBIOSE PHOSPHORYLASE, DIRECTED EVOLUTION, BIOTRANSFORMATION, PHOSPHATASE

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MLA
De Groeve, Manu et al. “Enzymatic Production of Alpha-d-galactose 1-phosphate by Lactose Phosphorolysis.” BIOTECHNOLOGY LETTERS 31.12 (2009): 1873–1877. Print.
APA
De Groeve, M., Depreitere, V., Desmet, T., & Soetaert, W. (2009). Enzymatic production of alpha-d-galactose 1-phosphate by lactose phosphorolysis. BIOTECHNOLOGY LETTERS, 31(12), 1873–1877.
Chicago author-date
De Groeve, Manu, Veerle Depreitere, Tom Desmet, and Wim Soetaert. 2009. “Enzymatic Production of Alpha-d-galactose 1-phosphate by Lactose Phosphorolysis.” Biotechnology Letters 31 (12): 1873–1877.
Chicago author-date (all authors)
De Groeve, Manu, Veerle Depreitere, Tom Desmet, and Wim Soetaert. 2009. “Enzymatic Production of Alpha-d-galactose 1-phosphate by Lactose Phosphorolysis.” Biotechnology Letters 31 (12): 1873–1877.
Vancouver
1.
De Groeve M, Depreitere V, Desmet T, Soetaert W. Enzymatic production of alpha-d-galactose 1-phosphate by lactose phosphorolysis. BIOTECHNOLOGY LETTERS. 2009;31(12):1873–7.
IEEE
[1]
M. De Groeve, V. Depreitere, T. Desmet, and W. Soetaert, “Enzymatic production of alpha-d-galactose 1-phosphate by lactose phosphorolysis,” BIOTECHNOLOGY LETTERS, vol. 31, no. 12, pp. 1873–1877, 2009.
@article{807924,
  abstract     = {alpha-d-Galactose 1-phosphate (alpha Gal1P) is an important building block for the synthesis of nucleotide sugars that are substrates for glycosyltransferases. We have previously reported the creation of novel lactose phosphorylase enzymes that are useful for the synthesis of alpha Gal1P from the cheap and abundant lactose. Here we describe the application of such a lactose phosphorylase in a production system using permeabilized Escherichia coli cells. After purification of the product from the reaction mixture by anion-exchange chromatography and ethanol precipitation, 9.5 grams of highly pure alpha Gal1P were obtained from a 1 l reaction volume.},
  author       = {De Groeve, Manu and Depreitere, Veerle and Desmet, Tom and Soetaert, Wim},
  issn         = {0141-5492},
  journal      = {BIOTECHNOLOGY LETTERS},
  keywords     = {Galactose 1-phosphate,Bioconversion,Lactose phosphorylase,Phosphorolysis,Purification,Synthesis,CELLOBIOSE PHOSPHORYLASE,DIRECTED EVOLUTION,BIOTRANSFORMATION,PHOSPHATASE},
  language     = {eng},
  number       = {12},
  pages        = {1873--1877},
  title        = {Enzymatic production of alpha-d-galactose 1-phosphate by lactose phosphorolysis},
  url          = {http://dx.doi.org/10.1007/s10529-009-0087-1},
  volume       = {31},
  year         = {2009},
}

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