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Non-instrumental immunochemical tests for rapid ochratoxin A detection in red wine

(2009) ANALYTICA CHIMICA ACTA. 653(1). p.97-102
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Abstract
Gel-based and membrane-based flow-through immunoassay formats were investigated for rapid ochratoxin A (OTA) detection in red wine. The flow-through set-Up consisted of an antibody containing gel or membrane placed at the bottom of a standard solid-phase extraction column (i.e the flow-through column), combined with a clean-up column. Different clean-up methods were studied for red wine clarification and purification The optimal method consisted of passing wine, diluted with an aqueous Solution containing 1% polyethylene glycol (PEG 6000) and 5% sodium hydrogencarbonate, through strong anion exchange (SAX) silica. An immunoassay for OTA detection in red wine was optimized and a cut-off level at 2 mu g L-1 according to EU legislation was achieved with both formals A more significant colour difference between blank and spiked samples was observed for the gel-based assay making this superior to the inembiane-based assay The proposed rapid gel-based test was compared with a standard immunoaffinity column - high-performance liquid chromatography - fluorescent detection (IAC-HPLC-FLD) method and a good correlation of the results was obtained for naturally contaminated wine samples
Keywords
IMMUNOASSAY, FLUORESCENCE DETECTION, SOLID-PHASE EXTRACTION, PERFORMANCE LIQUID-CHROMATOGRAPHY, IMMUNOAFFINITY COLUMN CLEANUP, BEER

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MLA
Rusanova, TY et al. “Non-instrumental Immunochemical Tests for Rapid Ochratoxin A Detection in Red Wine.” ANALYTICA CHIMICA ACTA 653.1 (2009): 97–102. Print.
APA
Rusanova, T., Beloglazova, N., Goryacheva, I., Lobeau, M., Van Peteghem, C., & De Saeger, S. (2009). Non-instrumental immunochemical tests for rapid ochratoxin A detection in red wine. ANALYTICA CHIMICA ACTA, 653(1), 97–102.
Chicago author-date
Rusanova, TY, NV Beloglazova, IY Goryacheva, M Lobeau, Carlos Van Peteghem, and Sarah De Saeger. 2009. “Non-instrumental Immunochemical Tests for Rapid Ochratoxin A Detection in Red Wine.” Analytica Chimica Acta 653 (1): 97–102.
Chicago author-date (all authors)
Rusanova, TY, NV Beloglazova, IY Goryacheva, M Lobeau, Carlos Van Peteghem, and Sarah De Saeger. 2009. “Non-instrumental Immunochemical Tests for Rapid Ochratoxin A Detection in Red Wine.” Analytica Chimica Acta 653 (1): 97–102.
Vancouver
1.
Rusanova T, Beloglazova N, Goryacheva I, Lobeau M, Van Peteghem C, De Saeger S. Non-instrumental immunochemical tests for rapid ochratoxin A detection in red wine. ANALYTICA CHIMICA ACTA. AMSTERDAM: Elsevier Science; 2009;653(1):97–102.
IEEE
[1]
T. Rusanova, N. Beloglazova, I. Goryacheva, M. Lobeau, C. Van Peteghem, and S. De Saeger, “Non-instrumental immunochemical tests for rapid ochratoxin A detection in red wine,” ANALYTICA CHIMICA ACTA, vol. 653, no. 1, pp. 97–102, 2009.
@article{807821,
  abstract     = {Gel-based and membrane-based flow-through immunoassay formats were investigated for rapid ochratoxin A (OTA) detection in red wine. The flow-through set-Up consisted of an antibody containing gel or membrane placed at the bottom of a standard solid-phase extraction column (i.e the flow-through column), combined with a clean-up column. Different clean-up methods were studied for red wine clarification and purification The optimal method consisted of passing wine, diluted with an aqueous Solution containing 1% polyethylene glycol (PEG 6000) and 5% sodium hydrogencarbonate, through strong anion exchange (SAX) silica. An immunoassay for OTA detection in red wine was optimized and a cut-off level at 2 mu g L-1 according to EU legislation was achieved with both formals A more significant colour difference between blank and spiked samples was observed for the gel-based assay making this superior to the inembiane-based assay The proposed rapid gel-based test was compared with a standard immunoaffinity column - high-performance liquid chromatography - fluorescent detection (IAC-HPLC-FLD) method and a good correlation of the results was obtained for naturally contaminated wine samples},
  author       = {Rusanova, TY and Beloglazova, NV and Goryacheva, IY and Lobeau, M and Van Peteghem, Carlos and De Saeger, Sarah},
  issn         = {0003-2670},
  journal      = {ANALYTICA CHIMICA ACTA},
  keywords     = {IMMUNOASSAY,FLUORESCENCE DETECTION,SOLID-PHASE EXTRACTION,PERFORMANCE LIQUID-CHROMATOGRAPHY,IMMUNOAFFINITY COLUMN CLEANUP,BEER},
  language     = {eng},
  number       = {1},
  pages        = {97--102},
  publisher    = {Elsevier Science},
  title        = {Non-instrumental immunochemical tests for rapid ochratoxin A detection in red wine},
  url          = {http://dx.doi.org/10.1016/j.aca.2009.08.036},
  volume       = {653},
  year         = {2009},
}

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