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Cosupplementation of isoflavones, prenylflavonoids, and lignans alters human exposure to phytoestrogen-derived 17beta-estradiol equivalents

Selin Bolca UGent, Ciska Wyns UGent, Sam Possemiers UGent, Herman Depypere UGent, Denis De Keukeleire UGent, Marc Bracke UGent, Willy Verstraete UGent and Arne Heyerick UGent (2009) JOURNAL OF NUTRITION. 139(12). p.2293-2300
abstract
The microbial metabolism of dietary phytoestrogens varies considerably among individuals and influences the final exposure to bioactive compounds. In view of the increasing number of food supplements combining several classes of phytoestrogens, the microbial potential to activate various proestrogens within an individual was evaluated in 3 randomized dietary crossovers. Treatment allocation was based on participants' eligibility (>45% in vitro bioactivation of >= 2 separate proestrogens by fecal cultures; n = 40/100). After a run-in of >= 4 d, participants were given soy-, hop-, and/or flax-based food supplements dosed either separately (SOY: 2.83 mg daidzein aglycone equivalents/supplement, HOP: 1.20 mg isoxanthohumol (IX)/supplement, or FLAX: 2.08 mg secoisolariciresinol (SECO) aglycone equivalents/supplement; reference intervention) or simultaneously (MIX; test intervention) 3 times/d for 5 cl, followed by a washout period l >= 7 d) and the second intervention. Before and after each (co)supplementation, spot urine and serum were collected. In total, 22 equol, 19 8-prenylnaringenin (8-PN), and 21 enterolactone (ENL) producers completed the SOY+MIX, HOP+MIX, and FLAX+MIX trials, respectively, The microbial bioactivation of daidzein, IX, and SECO, generally decreased upon coincubation in vitro (equol: 4.4%, P = 0.164; 8-PN: 20.5%, P < 0.001; ENL: 44.3%, P < 0.001) and cosupplementation in vivo (equol: 28.3%, P = 0.009; 8-PN: 35.4%, P = 0,107; ENL: 35.9%, P = 0.003). Although the bioavailabilities of total isoflavones, prenylflavonoids, and lignans were not significantly affected upon coadministration, participants were exposed to lower phytoestrogen-derived 17 beta-estradiol equivalents. In conclusion, the bioavailability of phytoestrogens, especially when given in mixtures, is subject to high interindividual variation. These findings support the importance of personalized screening when assessing the efficacy of such products and mixtures. J. Nutr. 139: 2293-2300,2009.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
EQUOL-PRODUCING BACTERIUM, HUMAN INTESTINAL BACTERIA, ESTROGEN-RECEPTOR-ALPHA, POSTMENOPAUSAL WOMEN, PHYTO-ESTROGENS, SECOISOLARICIRESINOL DIGLUCOSIDE, POTENT PHYTOESTROGEN, SYNTHETIC CHEMICALS, PRODUCER PHENOTYPE, HUMULUS-LUPULUS L.
journal title
JOURNAL OF NUTRITION
J. Nutr.
volume
139
issue
12
pages
2293 - 2300
Web of Science type
Article
Web of Science id
000272478900011
JCR category
NUTRITION & DIETETICS
JCR impact factor
4.091 (2009)
JCR rank
8/66 (2009)
JCR quartile
1 (2009)
ISSN
0022-3166
DOI
10.3945/jn.109.113639
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
807214
handle
http://hdl.handle.net/1854/LU-807214
date created
2009-12-11 10:44:26
date last changed
2010-01-08 16:01:37
@article{807214,
  abstract     = {The microbial metabolism of dietary phytoestrogens varies considerably among individuals and influences the final exposure to bioactive compounds. In view of the increasing number of food supplements combining several classes of phytoestrogens, the microbial potential to activate various proestrogens within an individual was evaluated in 3 randomized dietary crossovers. Treatment allocation was based on participants' eligibility ({\textrangle}45\% in vitro bioactivation of {\textrangle}= 2 separate proestrogens by fecal cultures; n = 40/100). After a run-in of {\textrangle}= 4 d, participants were given soy-, hop-, and/or flax-based food supplements dosed either separately (SOY: 2.83 mg daidzein aglycone equivalents/supplement, HOP: 1.20 mg isoxanthohumol (IX)/supplement, or FLAX: 2.08 mg secoisolariciresinol (SECO) aglycone equivalents/supplement; reference intervention) or simultaneously (MIX; test intervention) 3 times/d for 5 cl, followed by a washout period l {\textrangle}= 7 d) and the second intervention. Before and after each (co)supplementation, spot urine and serum were collected. In total, 22 equol, 19 8-prenylnaringenin (8-PN), and 21 enterolactone (ENL) producers completed the SOY+MIX, HOP+MIX, and FLAX+MIX trials, respectively, The microbial bioactivation of daidzein, IX, and SECO, generally decreased upon coincubation in vitro (equol: 4.4\%, P = 0.164; 8-PN: 20.5\%, P {\textlangle} 0.001; ENL: 44.3\%, P {\textlangle} 0.001) and cosupplementation in vivo (equol: 28.3\%, P = 0.009; 8-PN: 35.4\%, P = 0,107; ENL: 35.9\%, P = 0.003). Although the bioavailabilities of total isoflavones, prenylflavonoids, and lignans were not significantly affected upon coadministration, participants were exposed to lower phytoestrogen-derived 17 beta-estradiol equivalents. In conclusion, the bioavailability of phytoestrogens, especially when given in mixtures, is subject to high interindividual variation. These findings support the importance of personalized screening when assessing the efficacy of such products and mixtures. J. Nutr. 139: 2293-2300,2009.},
  author       = {Bolca, Selin and Wyns, Ciska and Possemiers, Sam and Depypere, Herman and De Keukeleire, Denis and Bracke, Marc and Verstraete, Willy and Heyerick, Arne},
  issn         = {0022-3166},
  journal      = {JOURNAL OF NUTRITION},
  keyword      = {EQUOL-PRODUCING BACTERIUM,HUMAN INTESTINAL BACTERIA,ESTROGEN-RECEPTOR-ALPHA,POSTMENOPAUSAL WOMEN,PHYTO-ESTROGENS,SECOISOLARICIRESINOL DIGLUCOSIDE,POTENT PHYTOESTROGEN,SYNTHETIC CHEMICALS,PRODUCER PHENOTYPE,HUMULUS-LUPULUS L.},
  language     = {eng},
  number       = {12},
  pages        = {2293--2300},
  title        = {Cosupplementation of isoflavones, prenylflavonoids, and lignans alters human exposure to phytoestrogen-derived 17beta-estradiol equivalents},
  url          = {http://dx.doi.org/10.3945/jn.109.113639},
  volume       = {139},
  year         = {2009},
}

Chicago
Bolca, Selin, Ciska Wyns, Sam Possemiers, Herman Depypere, Denis De Keukeleire, Marc Bracke, Willy Verstraete, and Arne Heyerick. 2009. “Cosupplementation of Isoflavones, Prenylflavonoids, and Lignans Alters Human Exposure to Phytoestrogen-derived 17beta-estradiol Equivalents.” Journal of Nutrition 139 (12): 2293–2300.
APA
Bolca, S., Wyns, C., Possemiers, S., Depypere, H., De Keukeleire, D., Bracke, M., Verstraete, W., et al. (2009). Cosupplementation of isoflavones, prenylflavonoids, and lignans alters human exposure to phytoestrogen-derived 17beta-estradiol equivalents. JOURNAL OF NUTRITION, 139(12), 2293–2300.
Vancouver
1.
Bolca S, Wyns C, Possemiers S, Depypere H, De Keukeleire D, Bracke M, et al. Cosupplementation of isoflavones, prenylflavonoids, and lignans alters human exposure to phytoestrogen-derived 17beta-estradiol equivalents. JOURNAL OF NUTRITION. 2009;139(12):2293–300.
MLA
Bolca, Selin, Ciska Wyns, Sam Possemiers, et al. “Cosupplementation of Isoflavones, Prenylflavonoids, and Lignans Alters Human Exposure to Phytoestrogen-derived 17beta-estradiol Equivalents.” JOURNAL OF NUTRITION 139.12 (2009): 2293–2300. Print.