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Toxin-neutralizing antibodies protect against Clostridium perfringens-induced necrosis in an intestinal loop model for bovine necrohemorrhagic enteritis

Evy Goossens UGent, Stefanie Verherstraeten, Bonnie Valgaeren, Bart Pardon UGent, Leen Timbermont, Stijn Schauvliege UGent, Diego Rodrigo, Freddy Haesebrouck UGent, Richard Ducatelle UGent, Piet Deprez UGent, et al. (2016) BMC VETERINARY RESEARCH. 12.
abstract
Background: Bovine necrohemorrhagic enteritis is caused by Clostridium perfringens type A. Due to the rapid progress and fatal outcome of the disease, vaccination would be of high value. In this study, C. perfringens toxins, either as native toxins or after formaldehyde inactivation, were evaluated as possible vaccine antigens. We determined whether antisera raised in calves against these toxins were able to protect against C. perfringens challenge in an intestinal loop model for bovine necrohemorrhagic enteritis. Results: Alpha toxin and perfringolysin O were identified as the most immunogenic proteins in the vaccine preparations. All vaccines evoked a high antibody response against the causative toxins, alpha toxin and perfringolysin O, as detected by ELISA. All antibodies were able to inhibit the activity of alpha toxin and perfringolysin O in vitro. However, the antibodies raised against the native toxins were more inhibitory to the C. perfringens-induced cytotoxicity (as tested on bovine endothelial cells) and only these antibodies protected against C. perfringens challenge in the intestinal loop model. Conclusion: Although immunization of calves with both native and formaldehyde inactivated toxins resulted in high antibody titers against alpha toxin and perfringolysin O, only antibodies raised against native toxins protect against C. perfringens challenge in an intestinal loop model for bovine necrohemorrhagic enteritis.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Clostridium perfringens, Bovine necrohemorrhagic enteritis, Neutralizing antibodies, Alpha toxin, Perfringolysin O, MEDIATED GAS-GANGRENE, ALPHA-TOXIN, ENDOTHELIAL-CELLS, PERFRINGOLYSIN-O, C. PERFRINGENS, VEAL CALVES, ENTEROTOXEMIA, IMMUNIZATION, VIRULENCE, INFECTIONS
journal title
BMC VETERINARY RESEARCH
BMC Vet. Res.
volume
12
article number
101
pages
8 pages
Web of Science type
Article
Web of Science id
000377650400002
JCR category
VETERINARY SCIENCES
JCR impact factor
1.75 (2016)
JCR rank
24/136 (2016)
JCR quartile
1 (2016)
ISSN
1746-6148
DOI
10.1186/s12917-016-0730-8
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
8068759
handle
http://hdl.handle.net/1854/LU-8068759
date created
2016-09-08 10:04:47
date last changed
2016-12-21 15:41:58
@article{8068759,
  abstract     = {Background: Bovine necrohemorrhagic enteritis is caused by Clostridium perfringens type A. Due to the rapid progress and fatal outcome of the disease, vaccination would be of high value. In this study, C. perfringens toxins, either as native toxins or after formaldehyde inactivation, were evaluated as possible vaccine antigens. We determined whether antisera raised in calves against these toxins were able to protect against C. perfringens challenge in an intestinal loop model for bovine necrohemorrhagic enteritis. 
Results: Alpha toxin and perfringolysin O were identified as the most immunogenic proteins in the vaccine preparations. All vaccines evoked a high antibody response against the causative toxins, alpha toxin and perfringolysin O, as detected by ELISA. All antibodies were able to inhibit the activity of alpha toxin and perfringolysin O in vitro. However, the antibodies raised against the native toxins were more inhibitory to the C. perfringens-induced cytotoxicity (as tested on bovine endothelial cells) and only these antibodies protected against C. perfringens challenge in the intestinal loop model. 
Conclusion: Although immunization of calves with both native and formaldehyde inactivated toxins resulted in high antibody titers against alpha toxin and perfringolysin O, only antibodies raised against native toxins protect against C. perfringens challenge in an intestinal loop model for bovine necrohemorrhagic enteritis.},
  articleno    = {101},
  author       = {Goossens, Evy and Verherstraeten, Stefanie and Valgaeren, Bonnie and Pardon, Bart and Timbermont, Leen and Schauvliege, Stijn and Rodrigo, Diego and Haesebrouck, Freddy and Ducatelle, Richard and Deprez, Piet and Van Immerseel, Filip},
  issn         = {1746-6148},
  journal      = {BMC VETERINARY RESEARCH},
  keyword      = {Clostridium perfringens,Bovine necrohemorrhagic enteritis,Neutralizing antibodies,Alpha toxin,Perfringolysin O,MEDIATED GAS-GANGRENE,ALPHA-TOXIN,ENDOTHELIAL-CELLS,PERFRINGOLYSIN-O,C. PERFRINGENS,VEAL CALVES,ENTEROTOXEMIA,IMMUNIZATION,VIRULENCE,INFECTIONS},
  language     = {eng},
  pages        = {8},
  title        = {Toxin-neutralizing antibodies protect against Clostridium perfringens-induced necrosis in an intestinal loop model for bovine necrohemorrhagic enteritis},
  url          = {http://dx.doi.org/10.1186/s12917-016-0730-8},
  volume       = {12},
  year         = {2016},
}

Chicago
Goossens, Evy, Stefanie Verherstraeten, Bonnie Valgaeren, Bart Pardon, Leen Timbermont, Stijn Schauvliege, Diego Rodrigo, et al. 2016. “Toxin-neutralizing Antibodies Protect Against Clostridium Perfringens-induced Necrosis in an Intestinal Loop Model for Bovine Necrohemorrhagic Enteritis.” Bmc Veterinary Research 12.
APA
Goossens, Evy, Verherstraeten, S., Valgaeren, B., Pardon, B., Timbermont, L., Schauvliege, S., Rodrigo, D., et al. (2016). Toxin-neutralizing antibodies protect against Clostridium perfringens-induced necrosis in an intestinal loop model for bovine necrohemorrhagic enteritis. BMC VETERINARY RESEARCH, 12.
Vancouver
1.
Goossens E, Verherstraeten S, Valgaeren B, Pardon B, Timbermont L, Schauvliege S, et al. Toxin-neutralizing antibodies protect against Clostridium perfringens-induced necrosis in an intestinal loop model for bovine necrohemorrhagic enteritis. BMC VETERINARY RESEARCH. 2016;12.
MLA
Goossens, Evy, Stefanie Verherstraeten, Bonnie Valgaeren, et al. “Toxin-neutralizing Antibodies Protect Against Clostridium Perfringens-induced Necrosis in an Intestinal Loop Model for Bovine Necrohemorrhagic Enteritis.” BMC VETERINARY RESEARCH 12 (2016): n. pag. Print.