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Protection of budgerigars (Melopsittacus undulatus) against Chlamydophila psittaci challenge by DNA vaccination

Taher Harkinezhad, Katelijn Schautteet UGent and Daisy Vanrompay UGent (2009) VETERINARY RESEARCH. 40(6).
abstract
Plasmid DNA (pcDNA1::MOMP A) expressing the major outer membrane protein (MOMP) of Chlamydophila psittaci genotype A strain 89/1051 has been tested for its ability to induce protective immunity against Cp. psittaci challenge in budgerigars. Eight pairs of male and female budgerigars were housed in eight separate bird cages placed in two negative pressure isolators, four cages per group. All budgerigars were immunised twice intramuscularly with 100 mu g plasmid DNA. Both groups received a primary DNA inoculation at day 0 followed by a booster inoculation 3 weeks later. Group 1 received pcDNA1:: MOMP A, while group 2 received the placebo vaccine pcDNA1. Budgerigars were challenged by aerosol 2 weeks following the booster vaccination. The challenge consisted of 10(8) TCID50 of the homologous Cp. psittaci genotype A strain. Cloacal and pharyngeal swabs of all budgerigars, taken prior to the experimental infection were negative in both PCR and culture. However, all budgerigars showed low pre-existing serum antibody titres. This indicates that animals were previously infected. Nevertheless, DNA immunisation could significantly reduce clinical signs, macroscopic lesions, pharyngeal and cloacal excretion as well as chlamydial replication, even in the presence of pre-existing serum antibodies, as compared to the placebo-vaccinated controls.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
GENOTYPE, OUTER-MEMBRANE PROTEIN, PARROTS, CHLAMYDIA-PSITTACI, TURKEYS, INFECTION, HUMANS, ANTIBODIES, TRANSMISSION, PET BIRDS, Chlamydophila psittaci, DNA vaccination, MOMP, budgerigar (Melopsittacus undulatus)
journal title
VETERINARY RESEARCH
Vet. Res.
volume
40
issue
6
Web of Science type
Article
Web of Science id
000272039500010
JCR category
VETERINARY SCIENCES
JCR impact factor
3.579 (2009)
JCR rank
1/141 (2009)
JCR quartile
1 (2009)
ISSN
0928-4249
DOI
10.1051/vetres/2009044
language
English
UGent publication?
yes
classification
A1
additional info
article no. 61 (11 p.)
copyright statement
I have transferred the copyright for this publication to the publisher
id
806798
handle
http://hdl.handle.net/1854/LU-806798
date created
2009-12-10 18:03:56
date last changed
2010-01-08 14:55:14
@article{806798,
  abstract     = {Plasmid DNA (pcDNA1::MOMP A) expressing the major outer membrane protein (MOMP) of Chlamydophila psittaci genotype A strain 89/1051 has been tested for its ability to induce protective immunity against Cp. psittaci challenge in budgerigars. Eight pairs of male and female budgerigars were housed in eight separate bird cages placed in two negative pressure isolators, four cages per group. All budgerigars were immunised twice intramuscularly with 100 mu g plasmid DNA. Both groups received a primary DNA inoculation at day 0 followed by a booster inoculation 3 weeks later. Group 1 received pcDNA1:: MOMP A, while group 2 received the placebo vaccine pcDNA1. Budgerigars were challenged by aerosol 2 weeks following the booster vaccination. The challenge consisted of 10(8) TCID50 of the homologous Cp. psittaci genotype A strain. Cloacal and pharyngeal swabs of all budgerigars, taken prior to the experimental infection were negative in both PCR and culture. However, all budgerigars showed low pre-existing serum antibody titres. This indicates that animals were previously infected. Nevertheless, DNA immunisation could significantly reduce clinical signs, macroscopic lesions, pharyngeal and cloacal excretion as well as chlamydial replication, even in the presence of pre-existing serum antibodies, as compared to the placebo-vaccinated controls.},
  author       = {Harkinezhad, Taher and Schautteet, Katelijn and Vanrompay, Daisy},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keyword      = {GENOTYPE,OUTER-MEMBRANE PROTEIN,PARROTS,CHLAMYDIA-PSITTACI,TURKEYS,INFECTION,HUMANS,ANTIBODIES,TRANSMISSION,PET BIRDS,Chlamydophila psittaci,DNA vaccination,MOMP,budgerigar (Melopsittacus undulatus)},
  language     = {eng},
  number       = {6},
  title        = {Protection of budgerigars (Melopsittacus undulatus) against Chlamydophila psittaci challenge by DNA vaccination},
  url          = {http://dx.doi.org/10.1051/vetres/2009044},
  volume       = {40},
  year         = {2009},
}

Chicago
Harkinezhad, Taher, Katelijn Schautteet, and Daisy Vanrompay. 2009. “Protection of Budgerigars (Melopsittacus Undulatus) Against Chlamydophila Psittaci Challenge by DNA Vaccination.” Veterinary Research 40 (6).
APA
Harkinezhad, T., Schautteet, K., & Vanrompay, D. (2009). Protection of budgerigars (Melopsittacus undulatus) against Chlamydophila psittaci challenge by DNA vaccination. VETERINARY RESEARCH, 40(6).
Vancouver
1.
Harkinezhad T, Schautteet K, Vanrompay D. Protection of budgerigars (Melopsittacus undulatus) against Chlamydophila psittaci challenge by DNA vaccination. VETERINARY RESEARCH. 2009;40(6).
MLA
Harkinezhad, Taher, Katelijn Schautteet, and Daisy Vanrompay. “Protection of Budgerigars (Melopsittacus Undulatus) Against Chlamydophila Psittaci Challenge by DNA Vaccination.” VETERINARY RESEARCH 40.6 (2009): n. pag. Print.