High resolution mu SPECT for brain activation analysis in small animals
- Author
- Steven Staelens (UGent) , Tine Wyckhuys (UGent) , Steven Deleye, Hans Hallez (UGent) , Stefaan Vandenberghe (UGent) , Bert Van Nieuwenhuyse and Kim Vonck
- Organization
- Abstract
- A stimulus on/off imaging study is often used to evaluate the brain's response to a presented visual, electrical or chemical trigger. Clinical software and human templates already exist but given the recent advent of ultrahigh resolution μ SPECT and μ PET, a larger need rises for a post processing platform to perform these subtraction molecular imaging studies also in small animals. We have designed such a multimodal framework to perform μ SPECT activation studies in rats thereby making use of μ CT and MRI for anatomical land marking. Our software solution is a combination of Amide, MRIcroN and a custom made Matlab implementation. We have studied the performance of different deep brain stimulations for which 6 rats were implanted with a multi-polar stimulation electrode in the right hippocampus. Each animal underwent a 99mTc-HMPAO μ SPECT with the Milabs U-SPECT-II and a μ CT scan with the GMI X-O CT before and after stimulation. Two line markers in oblique positions filled with low activity of 125I are used to register the μ SPECT and μ CT images. Afterwards the animals were sacrificed, their electrode was removed and a MRI scan was performed using the wrist coil of a Siemens Trio 3T. A semi-automated five step procedure delivers the activation map: (i) first the stimulus-on μ CT and the MRI are registered to the stimulus-off μ CT followed by (ii) the fusion of the off/on μ SPECT scans with their off/on μ CT counterparts. From the MRI, (iii) the rat brain is extracted, which is used as a mask for the calculation. Afterwards, (iv) both off/on μ SPECT scans are normalized and subtracted within this MRI brain mask. Finally, (v) the Z-score, representing the activation map, is achieved by dividing the result with the standard deviation of the masked stimulus-off μ SPECT. This semi-automated approach allows the experimental neu-roscientist to draw conclusions on the location, spatial extent and intensity of the small animal brains response to the stimulus.
- Keywords
- multipolar stimulation electrode, hippocampus, brain activation analysis, μ SPECT, image registration, single photon emission computed tomography, prosthetics, image resolution, biomedical MRI, biomedical electrodes, brain, computerised tomography, feature extraction, image fusion, Siemens Trio 3T, stimulus on/off imaging, MRI, μ CT
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-806673
- MLA
- Staelens, Steven, et al. “High Resolution Mu SPECT for Brain Activation Analysis in Small Animals.” IEEE Nuclear Science Symposium Conference Record, edited by Bo Yu, IEEE, 2009, pp. 2702–05, doi:10.1109/NSSMIC.2009.5401980.
- APA
- Staelens, S., Wyckhuys, T., Deleye, S., Hallez, H., Vandenberghe, S., Van Nieuwenhuyse, B., & Vonck, K. (2009). High resolution mu SPECT for brain activation analysis in small animals. In B. Yu (Ed.), IEEE Nuclear Science Symposium Conference Record (pp. 2702–2705). https://doi.org/10.1109/NSSMIC.2009.5401980
- Chicago author-date
- Staelens, Steven, Tine Wyckhuys, Steven Deleye, Hans Hallez, Stefaan Vandenberghe, Bert Van Nieuwenhuyse, and Kim Vonck. 2009. “High Resolution Mu SPECT for Brain Activation Analysis in Small Animals.” In IEEE Nuclear Science Symposium Conference Record, edited by Bo Yu, 2702–5. Piscataway, NJ, USA: IEEE. https://doi.org/10.1109/NSSMIC.2009.5401980.
- Chicago author-date (all authors)
- Staelens, Steven, Tine Wyckhuys, Steven Deleye, Hans Hallez, Stefaan Vandenberghe, Bert Van Nieuwenhuyse, and Kim Vonck. 2009. “High Resolution Mu SPECT for Brain Activation Analysis in Small Animals.” In IEEE Nuclear Science Symposium Conference Record, ed by. Bo Yu, 2702–2705. Piscataway, NJ, USA: IEEE. doi:10.1109/NSSMIC.2009.5401980.
- Vancouver
- 1.Staelens S, Wyckhuys T, Deleye S, Hallez H, Vandenberghe S, Van Nieuwenhuyse B, et al. High resolution mu SPECT for brain activation analysis in small animals. In: Yu B, editor. IEEE Nuclear Science Symposium Conference Record. Piscataway, NJ, USA: IEEE; 2009. p. 2702–5.
- IEEE
- [1]S. Staelens et al., “High resolution mu SPECT for brain activation analysis in small animals,” in IEEE Nuclear Science Symposium Conference Record, Orlando, FL, USA, 2009, pp. 2702–2705.
@inproceedings{806673, abstract = {{A stimulus on/off imaging study is often used to evaluate the brain's response to a presented visual, electrical or chemical trigger. Clinical software and human templates already exist but given the recent advent of ultrahigh resolution μ SPECT and μ PET, a larger need rises for a post processing platform to perform these subtraction molecular imaging studies also in small animals. We have designed such a multimodal framework to perform μ SPECT activation studies in rats thereby making use of μ CT and MRI for anatomical land marking. Our software solution is a combination of Amide, MRIcroN and a custom made Matlab implementation. We have studied the performance of different deep brain stimulations for which 6 rats were implanted with a multi-polar stimulation electrode in the right hippocampus. Each animal underwent a 99mTc-HMPAO μ SPECT with the Milabs U-SPECT-II and a μ CT scan with the GMI X-O CT before and after stimulation. Two line markers in oblique positions filled with low activity of 125I are used to register the μ SPECT and μ CT images. Afterwards the animals were sacrificed, their electrode was removed and a MRI scan was performed using the wrist coil of a Siemens Trio 3T. A semi-automated five step procedure delivers the activation map: (i) first the stimulus-on μ CT and the MRI are registered to the stimulus-off μ CT followed by (ii) the fusion of the off/on μ SPECT scans with their off/on μ CT counterparts. From the MRI, (iii) the rat brain is extracted, which is used as a mask for the calculation. Afterwards, (iv) both off/on μ SPECT scans are normalized and subtracted within this MRI brain mask. Finally, (v) the Z-score, representing the activation map, is achieved by dividing the result with the standard deviation of the masked stimulus-off μ SPECT. This semi-automated approach allows the experimental neu-roscientist to draw conclusions on the location, spatial extent and intensity of the small animal brains response to the stimulus.}}, author = {{Staelens, Steven and Wyckhuys, Tine and Deleye, Steven and Hallez, Hans and Vandenberghe, Stefaan and Van Nieuwenhuyse, Bert and Vonck, Kim}}, booktitle = {{IEEE Nuclear Science Symposium Conference Record}}, editor = {{Yu, Bo}}, isbn = {{9781424439614}}, issn = {{1082-3654}}, keywords = {{multipolar stimulation electrode,hippocampus,brain activation analysis,μ SPECT,image registration,single photon emission computed tomography,prosthetics,image resolution,biomedical MRI,biomedical electrodes,brain,computerised tomography,feature extraction,image fusion,Siemens Trio 3T,stimulus on/off imaging,MRI,μ CT}}, language = {{eng}}, location = {{Orlando, FL, USA}}, pages = {{2702--2705}}, publisher = {{IEEE}}, title = {{High resolution mu SPECT for brain activation analysis in small animals}}, url = {{http://doi.org/10.1109/NSSMIC.2009.5401980}}, year = {{2009}}, }
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