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Lactonic sophorolipids increase tumor burden in Apc(min+/-) mice

(2016) PLOS ONE. 11(6).
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Abstract
Sophorolipids (SL) are amphiphilic biosurfactant molecules consisting of a disaccharide sophorose with one fatty acid at the C1 position and optional acetylation at the C6' and C6 '' positions. They exist in a closed ring lactonic (LSL) or open acidic (ASL) structure Sophorolipids are produced in crude mixtures in economically viable amounts by the yeast Starmerella bombicola and used in a variety of consumer products. Varying levels of antiproliferative and anti-cancer activity of crude sophorolipid mixtures are described in a number of tumor cell lines in vitro. However, significant inter-study variation exists in the composition of sophorolipid species as well as other biologically active compounds in these mixtures, which makes interpretation of in vitro and in vivo studies difficult. We produced a 96% pure C18:1 lactonic sophorolipid that dose-dependently reduces the viability of colorectal cancer, as well as normal human colonic and lung cell lines in vitro. Oral administration of vehicle-only; or lactonic sophorolipids (50 mg/kg for 70 days), to Apcmin(+/-) mice resulted in an increase in the number (55.5 +/- 3.3 vs 70.50 +/- 7.8: p < 0.05) and size (modal size 2mm vs 4mm) of intestinal polyps. Lactonic administration resulted in a systematic effect via reduced hematocrit (49.5 +/- 1.0 vs 28.2 +/- 2.0 vs: p<0.03) and splenomegaly (0.56 +/- 0.03g vs 0.71 +/- 0.04g; p<0.01) confirming exacerbation of disease progression in this model.
Keywords
BIOSURFACTANTS, APOPTOSIS, APC GENE, FOOD-INDUSTRY, IGE PRODUCTION, MICROBIAL-PRODUCTION, CANCER CELLS, MODEL, MOUSE, SEPSIS

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Citation

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Chicago
Callaghan, Breedge, Helen Lydon, Sophie Roelants, Inge Van Bogaert, Roger Marchant, Ibrahim M Banat, and Christoper A Mitchell. 2016. “Lactonic Sophorolipids Increase Tumor Burden in Apc(min+/-) Mice.” Plos One 11 (6).
APA
Callaghan, B., Lydon, H., Roelants, S., Van Bogaert, I., Marchant, R., Banat, I. M., & Mitchell, C. A. (2016). Lactonic sophorolipids increase tumor burden in Apc(min+/-) mice. PLOS ONE, 11(6).
Vancouver
1.
Callaghan B, Lydon H, Roelants S, Van Bogaert I, Marchant R, Banat IM, et al. Lactonic sophorolipids increase tumor burden in Apc(min+/-) mice. PLOS ONE. 2016;11(6).
MLA
Callaghan, Breedge, Helen Lydon, Sophie Roelants, et al. “Lactonic Sophorolipids Increase Tumor Burden in Apc(min+/-) Mice.” PLOS ONE 11.6 (2016): n. pag. Print.
@article{8065795,
  abstract     = {Sophorolipids (SL) are amphiphilic biosurfactant molecules consisting of a disaccharide sophorose with one fatty acid at the C1 position and optional acetylation at the C6' and C6 '' positions. They exist in a closed ring lactonic (LSL) or open acidic (ASL) structure Sophorolipids are produced in crude mixtures in economically viable amounts by the yeast Starmerella bombicola and used in a variety of consumer products. Varying levels of antiproliferative and anti-cancer activity of crude sophorolipid mixtures are described in a number of tumor cell lines in vitro. However, significant inter-study variation exists in the composition of sophorolipid species as well as other biologically active compounds in these mixtures, which makes interpretation of in vitro and in vivo studies difficult. We produced a 96\% pure C18:1 lactonic sophorolipid that dose-dependently reduces the viability of colorectal cancer, as well as normal human colonic and lung cell lines in vitro. Oral administration of vehicle-only; or lactonic sophorolipids (50 mg/kg for 70 days), to Apcmin(+/-) mice resulted in an increase in the number (55.5 +/- 3.3 vs 70.50 +/- 7.8: p {\textlangle} 0.05) and size (modal size 2mm vs 4mm) of intestinal polyps. Lactonic administration resulted in a systematic effect via reduced hematocrit (49.5 +/- 1.0 vs 28.2 +/- 2.0 vs: p{\textlangle}0.03) and splenomegaly (0.56 +/- 0.03g vs 0.71 +/- 0.04g; p{\textlangle}0.01) confirming exacerbation of disease progression in this model.},
  articleno    = {e0156845},
  author       = {Callaghan, Breedge and Lydon, Helen and Roelants, Sophie and Van Bogaert, Inge and Marchant, Roger and Banat, Ibrahim M and Mitchell, Christoper A},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {BIOSURFACTANTS,APOPTOSIS,APC GENE,FOOD-INDUSTRY,IGE PRODUCTION,MICROBIAL-PRODUCTION,CANCER CELLS,MODEL,MOUSE,SEPSIS},
  language     = {eng},
  number       = {6},
  pages        = {16},
  title        = {Lactonic sophorolipids increase tumor burden in Apc(min+/-) mice},
  url          = {http://dx.doi.org/10.1371/journal.pone.0156845},
  volume       = {11},
  year         = {2016},
}

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