Ghent University Academic Bibliography

Advanced

Proteome of equine oviducal fluid : effects of ovulation and pregnancy

Katrien Smits UGent, Hilde Nelis, Katleen Van Steendam, Jan Govaere UGent, Kim Roels UGent, Cyrillus Ververs UGent, Bart Leemans UGent, Eline Wydooghe UGent, Dieter Deforce UGent and Ann Van Soom UGent (2017) REPRODUCTION FERTILITY AND DEVELOPMENT. 29(6). p.1085-1095
abstract
The equine oviduct plays a pivotal role in providing the optimal microenvironment for early embryonic development, but little is known about the protein composition of the oviducal fluid in the horse. The aim of the present study was to provide a large-scale identification of proteins in equine oviducal fluid and to determine the effects of ovulation and pregnancy. Four days after ovulation, the oviducts ipsilateral and contralateral to the ovulation side were collected from five pregnant and five non-pregnant mares. Identification and relative quantification of proteins in the oviducal fluid of the four groups was achieved by isobaric tags for relative and absolute quantification (iTRAQ) labelling and HPLC-tandem mass spectrometry. The presence of an embryo in the ipsilateral oviducal fluid of pregnant mares induced upregulation of 11 and downregulation of two proteins compared with the contralateral side, and upregulation of 19 proteins compared with the ipsilateral side of non-pregnant mares. Several of these upregulated proteins are related to early pregnancy in other species. The present study represents the first high-throughput identification of proteins in the oviducal fluid of the mare. The results support the hypothesis that the equine embryo interacts with the oviduct, affecting the maternal secretion pattern of proteins involved in pregnancy-related pathways.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
embryo, horse, oviduct, proteomics, UTERINE TUBAL FLUID, BRAIN-TUMOR 1, IN-VITRO, BLASTOCYST DEVELOPMENT, EARLY EMBRYO, PHOSPHOLIPASE-A2 ACTIVITY, UTEROGLOBIN, PROTEINS, UTERUS, HORSE
journal title
REPRODUCTION FERTILITY AND DEVELOPMENT
Reprod. Fertil. Dev.
volume
29
issue
6
pages
1085 - 1095
Web of Science type
Article
Web of Science id
000401583900004
ISSN
1031-3613
DOI
10.1071/RD15481
language
English
UGent publication?
yes
classification
A1
additional info
the first two authors contributed equally to this paper
copyright statement
I have transferred the copyright for this publication to the publisher
id
8055500
handle
http://hdl.handle.net/1854/LU-8055500
date created
2016-08-26 15:31:40
date last changed
2017-06-12 10:27:45
@article{8055500,
  abstract     = {The equine oviduct plays a pivotal role in providing the optimal microenvironment for early embryonic development, but little is known about the protein composition of the oviducal fluid in the horse. The aim of the present study was to provide a large-scale identification of proteins in equine oviducal fluid and to determine the effects of ovulation and pregnancy. Four days after ovulation, the oviducts ipsilateral and contralateral to the ovulation side were collected from five pregnant and five non-pregnant mares. Identification and relative quantification of proteins in the oviducal fluid of the four groups was achieved by isobaric tags for relative and absolute quantification (iTRAQ) labelling and HPLC-tandem mass spectrometry. The presence of an embryo in the ipsilateral oviducal fluid of pregnant mares induced upregulation of 11 and downregulation of two proteins compared with the contralateral side, and upregulation of 19 proteins compared with the ipsilateral side of non-pregnant mares. Several of these upregulated proteins are related to early pregnancy in other species. The present study represents the first high-throughput identification of proteins in the oviducal fluid of the mare. The results support the hypothesis that the equine embryo interacts with the oviduct, affecting the maternal secretion pattern of proteins involved in pregnancy-related pathways.},
  author       = {Smits, Katrien and Nelis, Hilde and Van Steendam, Katleen and Govaere, Jan and Roels, Kim and Ververs, Cyrillus and Leemans, Bart and Wydooghe, Eline and Deforce, Dieter and Van Soom, Ann},
  issn         = {1031-3613},
  journal      = {REPRODUCTION FERTILITY AND DEVELOPMENT},
  keyword      = {embryo,horse,oviduct,proteomics,UTERINE TUBAL FLUID,BRAIN-TUMOR 1,IN-VITRO,BLASTOCYST DEVELOPMENT,EARLY EMBRYO,PHOSPHOLIPASE-A2 ACTIVITY,UTEROGLOBIN,PROTEINS,UTERUS,HORSE},
  language     = {eng},
  number       = {6},
  pages        = {1085--1095},
  title        = {Proteome of equine oviducal fluid : effects of ovulation and pregnancy},
  url          = {http://dx.doi.org/10.1071/RD15481},
  volume       = {29},
  year         = {2017},
}

Chicago
Smits, Katrien, Hilde Nelis, Katleen Van Steendam, Jan Govaere, Kim Roels, Cyrillus Ververs, Bart Leemans, Eline Wydooghe, Dieter Deforce, and Ann Van Soom. 2017. “Proteome of Equine Oviducal Fluid : Effects of Ovulation and Pregnancy.” Reproduction Fertility and Development 29 (6): 1085–1095.
APA
Smits, Katrien, Nelis, H., Van Steendam, K., Govaere, J., Roels, K., Ververs, C., Leemans, B., et al. (2017). Proteome of equine oviducal fluid : effects of ovulation and pregnancy. REPRODUCTION FERTILITY AND DEVELOPMENT, 29(6), 1085–1095.
Vancouver
1.
Smits K, Nelis H, Van Steendam K, Govaere J, Roels K, Ververs C, et al. Proteome of equine oviducal fluid : effects of ovulation and pregnancy. REPRODUCTION FERTILITY AND DEVELOPMENT. 2017;29(6):1085–95.
MLA
Smits, Katrien, Hilde Nelis, Katleen Van Steendam, et al. “Proteome of Equine Oviducal Fluid : Effects of Ovulation and Pregnancy.” REPRODUCTION FERTILITY AND DEVELOPMENT 29.6 (2017): 1085–1095. Print.