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Distribution of the NF-kappa B Complex in the Inflammatory Exudates Characterizing the Idiopathic Inflammatory Myopathies

Kim Creus (UGent) , Boel De Paepe (UGent) , BART WERBROUCK (UGent) , VEERLE VERVAET (UGent) , J Weis and Jan De Bleecker (UGent)
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Abstract
The transcription factor nuclear factor-kappa B (NF-kappa B) is a ubiquitously expressed protein family that is considered crucial in autoimmunity. We describe NF-kappa B p50 and p65, and the inhibitor I-kappa B alpha in the inflammatory exudates characteristic for the different idiopathic inflammatory myopathies (IIM), that is, endomysial CD8(+) cytotoxic T cells invading non-necrotic fibers in polymyositis (PM) and sporadic inclusion body myositis (sIBM), and the perimysial/perivascular CD20(+) B cells and CD4(+) T cells in dermatomyositis (DM). We also analyzed other inflammatory cells in the vicinity of active inflammation sites. Strikingly, actively invading CD4(+) cells in PM and sIBM contained both p65 and p50, whereas I-kappa B alpha was absent. This could point to a high activation state in which these cells are capable of expressing a variety of inflammatory mediators, contributing to the degradation of non-necrotic fibers. Secondly, CD68(+) macrophages in the infiltrates in all three IIM subtypes showed strong nuclear p50 and I-kappa B alpha staining. This may point to a role for p50 in counteracting inflammation, a reaction that could be enhanced by an upregulation of I-kappa B alpha as we observed with immunofluorescence. These results shed further light on the immunopathology of PM, sIBM and DM. CD4(+) and CD68(+) mononuclear cells may play a more prominent role than previously assumed.
Keywords
DENDRITIC CELLS, T-CELLS, EXPRESSION, RELA, TRANSCRIPTION, REQUIREMENT, POLYMYOSITIS, NECROSIS-FACTOR-ALPHA, DUCHENNE DYSTROPHY, INCLUSION-BODY MYOSITIS

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Chicago
Creus, Kim, Boel De Paepe, BART WERBROUCK, VEERLE VERVAET, J Weis, and Jan De Bleecker. 2009. “Distribution of the NF-kappa B Complex in the Inflammatory Exudates Characterizing the Idiopathic Inflammatory Myopathies.” Annals of the New York Academy of Sciences 1173: 370–377.
APA
Creus, K., De Paepe, B., WERBROUCK, B., VERVAET, V., Weis, J., & De Bleecker, J. (2009). Distribution of the NF-kappa B Complex in the Inflammatory Exudates Characterizing the Idiopathic Inflammatory Myopathies. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1173, 370–377. Presented at the 6th International Congress on Autoimmunity.
Vancouver
1.
Creus K, De Paepe B, WERBROUCK B, VERVAET V, Weis J, De Bleecker J. Distribution of the NF-kappa B Complex in the Inflammatory Exudates Characterizing the Idiopathic Inflammatory Myopathies. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES. OXFORD: BLACKWELL PUBLISHING; 2009;1173:370–7.
MLA
Creus, Kim, Boel De Paepe, BART WERBROUCK, et al. “Distribution of the NF-kappa B Complex in the Inflammatory Exudates Characterizing the Idiopathic Inflammatory Myopathies.” ANNALS OF THE NEW YORK ACADEMY OF SCIENCES 1173 (2009): 370–377. Print.
@article{805402,
  abstract     = {The transcription factor nuclear factor-kappa B (NF-kappa B) is a ubiquitously expressed protein family that is considered crucial in autoimmunity. We describe NF-kappa B p50 and p65, and the inhibitor I-kappa B alpha in the inflammatory exudates characteristic for the different idiopathic inflammatory myopathies (IIM), that is, endomysial CD8(+) cytotoxic T cells invading non-necrotic fibers in polymyositis (PM) and sporadic inclusion body myositis (sIBM), and the perimysial/perivascular CD20(+) B cells and CD4(+) T cells in dermatomyositis (DM). We also analyzed other inflammatory cells in the vicinity of active inflammation sites. Strikingly, actively invading CD4(+) cells in PM and sIBM contained both p65 and p50, whereas I-kappa B alpha was absent. This could point to a high activation state in which these cells are capable of expressing a variety of inflammatory mediators, contributing to the degradation of non-necrotic fibers. Secondly, CD68(+) macrophages in the infiltrates in all three IIM subtypes showed strong nuclear p50 and I-kappa B alpha staining. This may point to a role for p50 in counteracting inflammation, a reaction that could be enhanced by an upregulation of I-kappa B alpha as we observed with immunofluorescence. These results shed further light on the immunopathology of PM, sIBM and DM. CD4(+) and CD68(+) mononuclear cells may play a more prominent role than previously assumed.},
  author       = {Creus, Kim and De Paepe, Boel and WERBROUCK, BART and VERVAET, VEERLE and Weis, J and De Bleecker, Jan},
  isbn         = {978-1-57331-762-7},
  issn         = {0077-8923},
  journal      = {ANNALS OF THE NEW YORK ACADEMY OF SCIENCES},
  language     = {eng},
  location     = {Oporto, PORTUGAL},
  pages        = {370--377},
  publisher    = {BLACKWELL PUBLISHING},
  title        = {Distribution of the NF-kappa B Complex in the Inflammatory Exudates Characterizing the Idiopathic Inflammatory Myopathies},
  url          = {http://dx.doi.org/10.1111/j.1749-6632.2009.04874.x},
  volume       = {1173},
  year         = {2009},
}

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