Distribution of the NF-kappa B Complex in the Inflammatory Exudates Characterizing the Idiopathic Inflammatory Myopathies
- Author
- Kim Creus (UGent) , Boel De Paepe (UGent) , BART WERBROUCK (UGent) , Veerle Vervaet, J Weis and Jan De Bleecker (UGent)
- Organization
- Abstract
- The transcription factor nuclear factor-kappa B (NF-kappa B) is a ubiquitously expressed protein family that is considered crucial in autoimmunity. We describe NF-kappa B p50 and p65, and the inhibitor I-kappa B alpha in the inflammatory exudates characteristic for the different idiopathic inflammatory myopathies (IIM), that is, endomysial CD8(+) cytotoxic T cells invading non-necrotic fibers in polymyositis (PM) and sporadic inclusion body myositis (sIBM), and the perimysial/perivascular CD20(+) B cells and CD4(+) T cells in dermatomyositis (DM). We also analyzed other inflammatory cells in the vicinity of active inflammation sites. Strikingly, actively invading CD4(+) cells in PM and sIBM contained both p65 and p50, whereas I-kappa B alpha was absent. This could point to a high activation state in which these cells are capable of expressing a variety of inflammatory mediators, contributing to the degradation of non-necrotic fibers. Secondly, CD68(+) macrophages in the infiltrates in all three IIM subtypes showed strong nuclear p50 and I-kappa B alpha staining. This may point to a role for p50 in counteracting inflammation, a reaction that could be enhanced by an upregulation of I-kappa B alpha as we observed with immunofluorescence. These results shed further light on the immunopathology of PM, sIBM and DM. CD4(+) and CD68(+) mononuclear cells may play a more prominent role than previously assumed.
- Keywords
- DENDRITIC CELLS, T-CELLS, EXPRESSION, RELA, TRANSCRIPTION, REQUIREMENT, POLYMYOSITIS, NECROSIS-FACTOR-ALPHA, DUCHENNE DYSTROPHY, INCLUSION-BODY MYOSITIS
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-805402
- MLA
- Creus, Kim, et al. “Distribution of the NF-Kappa B Complex in the Inflammatory Exudates Characterizing the Idiopathic Inflammatory Myopathies.” ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, vol. 1173, BLACKWELL PUBLISHING, 2009, pp. 370–77, doi:10.1111/j.1749-6632.2009.04874.x.
- APA
- Creus, K., De Paepe, B., WERBROUCK, B., Vervaet, V., Weis, J., & De Bleecker, J. (2009). Distribution of the NF-kappa B Complex in the Inflammatory Exudates Characterizing the Idiopathic Inflammatory Myopathies. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1173, 370–377. https://doi.org/10.1111/j.1749-6632.2009.04874.x
- Chicago author-date
- Creus, Kim, Boel De Paepe, BART WERBROUCK, Veerle Vervaet, J Weis, and Jan De Bleecker. 2009. “Distribution of the NF-Kappa B Complex in the Inflammatory Exudates Characterizing the Idiopathic Inflammatory Myopathies.” ANNALS OF THE NEW YORK ACADEMY OF SCIENCES 1173: 370–77. https://doi.org/10.1111/j.1749-6632.2009.04874.x.
- Chicago author-date (all authors)
- Creus, Kim, Boel De Paepe, BART WERBROUCK, Veerle Vervaet, J Weis, and Jan De Bleecker. 2009. “Distribution of the NF-Kappa B Complex in the Inflammatory Exudates Characterizing the Idiopathic Inflammatory Myopathies.” ANNALS OF THE NEW YORK ACADEMY OF SCIENCES 1173: 370–377. doi:10.1111/j.1749-6632.2009.04874.x.
- Vancouver
- 1.Creus K, De Paepe B, WERBROUCK B, Vervaet V, Weis J, De Bleecker J. Distribution of the NF-kappa B Complex in the Inflammatory Exudates Characterizing the Idiopathic Inflammatory Myopathies. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES. 2009;1173:370–7.
- IEEE
- [1]K. Creus, B. De Paepe, B. WERBROUCK, V. Vervaet, J. Weis, and J. De Bleecker, “Distribution of the NF-kappa B Complex in the Inflammatory Exudates Characterizing the Idiopathic Inflammatory Myopathies,” ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, vol. 1173, pp. 370–377, 2009.
@article{805402, abstract = {{The transcription factor nuclear factor-kappa B (NF-kappa B) is a ubiquitously expressed protein family that is considered crucial in autoimmunity. We describe NF-kappa B p50 and p65, and the inhibitor I-kappa B alpha in the inflammatory exudates characteristic for the different idiopathic inflammatory myopathies (IIM), that is, endomysial CD8(+) cytotoxic T cells invading non-necrotic fibers in polymyositis (PM) and sporadic inclusion body myositis (sIBM), and the perimysial/perivascular CD20(+) B cells and CD4(+) T cells in dermatomyositis (DM). We also analyzed other inflammatory cells in the vicinity of active inflammation sites. Strikingly, actively invading CD4(+) cells in PM and sIBM contained both p65 and p50, whereas I-kappa B alpha was absent. This could point to a high activation state in which these cells are capable of expressing a variety of inflammatory mediators, contributing to the degradation of non-necrotic fibers. Secondly, CD68(+) macrophages in the infiltrates in all three IIM subtypes showed strong nuclear p50 and I-kappa B alpha staining. This may point to a role for p50 in counteracting inflammation, a reaction that could be enhanced by an upregulation of I-kappa B alpha as we observed with immunofluorescence. These results shed further light on the immunopathology of PM, sIBM and DM. CD4(+) and CD68(+) mononuclear cells may play a more prominent role than previously assumed.}}, author = {{Creus, Kim and De Paepe, Boel and WERBROUCK, BART and Vervaet, Veerle and Weis, J and De Bleecker, Jan}}, isbn = {{978-1-57331-762-7}}, issn = {{0077-8923}}, journal = {{ANNALS OF THE NEW YORK ACADEMY OF SCIENCES}}, keywords = {{DENDRITIC CELLS,T-CELLS,EXPRESSION,RELA,TRANSCRIPTION,REQUIREMENT,POLYMYOSITIS,NECROSIS-FACTOR-ALPHA,DUCHENNE DYSTROPHY,INCLUSION-BODY MYOSITIS}}, language = {{eng}}, location = {{Oporto, PORTUGAL}}, pages = {{370--377}}, publisher = {{BLACKWELL PUBLISHING}}, title = {{Distribution of the NF-kappa B Complex in the Inflammatory Exudates Characterizing the Idiopathic Inflammatory Myopathies}}, url = {{http://doi.org/10.1111/j.1749-6632.2009.04874.x}}, volume = {{1173}}, year = {{2009}}, }
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