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RNA expression signatures and posttranscriptional regulation in diabetic nephropathy

(2015) NEPHROLOGY DIALYSIS TRANSPLANTATION. 30(suppl. 4). p.35-42
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Abstract
In the last decade, the integration of molecular approaches including transcriptome and miRNome analyses uncovered pathological mechanisms involved in the progression of diabetic nephropathy (DN). Using these techniques, molecular marker candidates [both messenger RNA (mRNA) and miRNA] have also been identified which may enable the characterization of patients at high risk for progression to end-stage renal disease. The results of such studies are urgently needed for a molecular definition of DN and for targeted treatment to improve patient care. The heterogeneity of kidney tissue and the minute amounts of RNA isolated from renal biopsies remain a challenge for omics-studies. Nevertheless, several studies have succeeded in the identification of RNA expression signatures in patients with diabetes and kidney disease. These studies show a reduced expression of growth factors such as VEGF and EGF, and an increased expression of matrix components and matrix-modulating enzymes, an activation of specific NF-kappa B modules, inflammatory pathways and the complement system. microRNAs are involved in the fine-tuning of mRNA abundance by binding to the 30 untranslated region of a target mRNA, which leads in most cases to translational repression or mRNA cleavage and a decrease in protein output. Here, we review the platforms used for miRNA expression profiling and ways to predict miRNA targets and functions. Several miRNAs have been shown to be involved in the pathogenesis of DN (e.g. miR-21, miR-192, miR-215, miR-216a, miR-29, let-7, miR-25, miR-93, etc.). Functional studies provide evidence that miRNAs are not only diagnostic tools but also represent potential therapeutic targets in DN.
Keywords
NONCODING RNAS, RENAL FIBROSIS, FACTOR-BETA, FUNCTIONAL ANNOTATION, MOLECULAR-MECHANISMS, gene expression, ENDOTHELIAL GROWTH-FACTOR, MICRORNA TARGET PREDICTION, TUBULAR EPITHELIAL-CELLS, diabetes mellitus, diabetic kidney disease, chronic renal insufficiency, diabetic nephropathy, GENE-EXPRESSION, VEGF-A EXPRESSION

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Citation

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MLA
Rudnicki, Michael et al. “RNA Expression Signatures and Posttranscriptional Regulation in Diabetic Nephropathy.” NEPHROLOGY DIALYSIS TRANSPLANTATION 30.suppl. 4 (2015): 35–42. Print.
APA
Rudnicki, M., Beckers, A., Neuwirt, H., & Vandesompele, J. (2015). RNA expression signatures and posttranscriptional regulation in diabetic nephropathy. NEPHROLOGY DIALYSIS TRANSPLANTATION, 30(suppl. 4), 35–42.
Chicago author-date
Rudnicki, Michael, Anneleen Beckers, Hannes Neuwirt, and Jo Vandesompele. 2015. “RNA Expression Signatures and Posttranscriptional Regulation in Diabetic Nephropathy.” Nephrology Dialysis Transplantation 30 (suppl. 4): 35–42.
Chicago author-date (all authors)
Rudnicki, Michael, Anneleen Beckers, Hannes Neuwirt, and Jo Vandesompele. 2015. “RNA Expression Signatures and Posttranscriptional Regulation in Diabetic Nephropathy.” Nephrology Dialysis Transplantation 30 (suppl. 4): 35–42.
Vancouver
1.
Rudnicki M, Beckers A, Neuwirt H, Vandesompele J. RNA expression signatures and posttranscriptional regulation in diabetic nephropathy. NEPHROLOGY DIALYSIS TRANSPLANTATION. 2015;30(suppl. 4):35–42.
IEEE
[1]
M. Rudnicki, A. Beckers, H. Neuwirt, and J. Vandesompele, “RNA expression signatures and posttranscriptional regulation in diabetic nephropathy,” NEPHROLOGY DIALYSIS TRANSPLANTATION, vol. 30, no. suppl. 4, pp. 35–42, 2015.
@article{8053643,
  abstract     = {In the last decade, the integration of molecular approaches including transcriptome and miRNome analyses uncovered pathological mechanisms involved in the progression of diabetic nephropathy (DN). Using these techniques, molecular marker candidates [both messenger RNA (mRNA) and miRNA] have also been identified which may enable the characterization of patients at high risk for progression to end-stage renal disease. The results of such studies are urgently needed for a molecular definition of DN and for targeted treatment to improve patient care. The heterogeneity of kidney tissue and the minute amounts of RNA isolated from renal biopsies remain a challenge for omics-studies. Nevertheless, several studies have succeeded in the identification of RNA expression signatures in patients with diabetes and kidney disease. These studies show a reduced expression of growth factors such as VEGF and EGF, and an increased expression of matrix components and matrix-modulating enzymes, an activation of specific NF-kappa B modules, inflammatory pathways and the complement system. microRNAs are involved in the fine-tuning of mRNA abundance by binding to the 30 untranslated region of a target mRNA, which leads in most cases to translational repression or mRNA cleavage and a decrease in protein output. Here, we review the platforms used for miRNA expression profiling and ways to predict miRNA targets and functions. Several miRNAs have been shown to be involved in the pathogenesis of DN (e.g. miR-21, miR-192, miR-215, miR-216a, miR-29, let-7, miR-25, miR-93, etc.). Functional studies provide evidence that miRNAs are not only diagnostic tools but also represent potential therapeutic targets in DN.},
  author       = {Rudnicki, Michael and Beckers, Anneleen and Neuwirt, Hannes and Vandesompele, Jo},
  issn         = {0931-0509},
  journal      = {NEPHROLOGY DIALYSIS TRANSPLANTATION},
  keywords     = {NONCODING RNAS,RENAL FIBROSIS,FACTOR-BETA,FUNCTIONAL ANNOTATION,MOLECULAR-MECHANISMS,gene expression,ENDOTHELIAL GROWTH-FACTOR,MICRORNA TARGET PREDICTION,TUBULAR EPITHELIAL-CELLS,diabetes mellitus,diabetic kidney disease,chronic renal insufficiency,diabetic nephropathy,GENE-EXPRESSION,VEGF-A EXPRESSION},
  language     = {eng},
  number       = {suppl. 4},
  pages        = {35--42},
  title        = {RNA expression signatures and posttranscriptional regulation in diabetic nephropathy},
  url          = {http://dx.doi.org/10.1093/ndt/gfv079},
  volume       = {30},
  year         = {2015},
}

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