Identification of individual exosome-like vesicles by surface enhanced Raman spectroscopy
- Author
- Stephan Stremersch (UGent) , Monica Marro, Bat-El Pinchasik, Pieter Baatsen, An Hendrix (UGent) , Stefaan De Smedt (UGent) , Pablo Loza-Alvarez, Andre Skirtach (UGent) , Koen Raemdonck (UGent) and Kevin Braeckmans (UGent)
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- Abstract
- Exosome-like vesicles (ELVs) are a novel class of biomarkers that are receiving a lot of attention for the detection of cancer at an early stage. In this study the feasibility of using a surface enhanced Raman spectroscopy (SERS) based method to distinguish between ELVs derived from different cellular origins is evaluated. A gold nanoparticle based shell is deposited on the surface of ELVs derived from cancerous and healthy cells, which enhances the Raman signal while maintaining a colloidal suspension of individual vesicles. This nanocoating allows the recording of SERS spectra from single vesicles. By using partial least squares discriminant analysis on the obtained spectra, vesicles from different origin can be distinguished, even when present in the same mixture. This proof-of-concept study paves the way for noninvasive (cancer) diagnostic tools based on exosomal SERS fingerprinting in combination with multivariate statistical analysis.
- Keywords
- RED-BLOOD-CELLS, EXTRACELLULAR VESICLES, BIOANALYTICAL APPLICATIONS, GOLD NANOPARTICLES, SINGLE-MOLECULE, LIVING CELLS, SERS, SCATTERING, PROTEINS, CANCER
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8051434
- MLA
- Stremersch, Stephan, et al. “Identification of Individual Exosome-like Vesicles by Surface Enhanced Raman Spectroscopy.” SMALL, vol. 12, no. 24, 2016, pp. 3292–301, doi:10.1002/smll.201600393.
- APA
- Stremersch, S., Marro, M., Pinchasik, B.-E., Baatsen, P., Hendrix, A., De Smedt, S., … Braeckmans, K. (2016). Identification of individual exosome-like vesicles by surface enhanced Raman spectroscopy. SMALL, 12(24), 3292–3301. https://doi.org/10.1002/smll.201600393
- Chicago author-date
- Stremersch, Stephan, Monica Marro, Bat-El Pinchasik, Pieter Baatsen, An Hendrix, Stefaan De Smedt, Pablo Loza-Alvarez, Andre Skirtach, Koen Raemdonck, and Kevin Braeckmans. 2016. “Identification of Individual Exosome-like Vesicles by Surface Enhanced Raman Spectroscopy.” SMALL 12 (24): 3292–3301. https://doi.org/10.1002/smll.201600393.
- Chicago author-date (all authors)
- Stremersch, Stephan, Monica Marro, Bat-El Pinchasik, Pieter Baatsen, An Hendrix, Stefaan De Smedt, Pablo Loza-Alvarez, Andre Skirtach, Koen Raemdonck, and Kevin Braeckmans. 2016. “Identification of Individual Exosome-like Vesicles by Surface Enhanced Raman Spectroscopy.” SMALL 12 (24): 3292–3301. doi:10.1002/smll.201600393.
- Vancouver
- 1.Stremersch S, Marro M, Pinchasik B-E, Baatsen P, Hendrix A, De Smedt S, et al. Identification of individual exosome-like vesicles by surface enhanced Raman spectroscopy. SMALL. 2016;12(24):3292–301.
- IEEE
- [1]S. Stremersch et al., “Identification of individual exosome-like vesicles by surface enhanced Raman spectroscopy,” SMALL, vol. 12, no. 24, pp. 3292–3301, 2016.
@article{8051434, abstract = {{Exosome-like vesicles (ELVs) are a novel class of biomarkers that are receiving a lot of attention for the detection of cancer at an early stage. In this study the feasibility of using a surface enhanced Raman spectroscopy (SERS) based method to distinguish between ELVs derived from different cellular origins is evaluated. A gold nanoparticle based shell is deposited on the surface of ELVs derived from cancerous and healthy cells, which enhances the Raman signal while maintaining a colloidal suspension of individual vesicles. This nanocoating allows the recording of SERS spectra from single vesicles. By using partial least squares discriminant analysis on the obtained spectra, vesicles from different origin can be distinguished, even when present in the same mixture. This proof-of-concept study paves the way for noninvasive (cancer) diagnostic tools based on exosomal SERS fingerprinting in combination with multivariate statistical analysis.}}, author = {{Stremersch, Stephan and Marro, Monica and Pinchasik, Bat-El and Baatsen, Pieter and Hendrix, An and De Smedt, Stefaan and Loza-Alvarez, Pablo and Skirtach, Andre and Raemdonck, Koen and Braeckmans, Kevin}}, issn = {{1613-6810}}, journal = {{SMALL}}, keywords = {{RED-BLOOD-CELLS,EXTRACELLULAR VESICLES,BIOANALYTICAL APPLICATIONS,GOLD NANOPARTICLES,SINGLE-MOLECULE,LIVING CELLS,SERS,SCATTERING,PROTEINS,CANCER}}, language = {{eng}}, number = {{24}}, pages = {{3292--3301}}, title = {{Identification of individual exosome-like vesicles by surface enhanced Raman spectroscopy}}, url = {{http://doi.org/10.1002/smll.201600393}}, volume = {{12}}, year = {{2016}}, }
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