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Impact of detergent-based decellularization methods on porcine tissues for heart valve engineering

(2016) ANNALS OF BIOMEDICAL ENGINEERING. 44(9). p.2827-2839
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Abstract
To date an optimal decellularization protocol of heart valve leaflets (HVL) and pericardia (PER) with an adequate preservation of the extracellular matrix (ECM) is still lacking. This study compares a 4 day Triton X-100-based protocol with faster SDC-based protocols for the decellularization of cardiac tissues. Decellularized and non-treated HVL and PER were processed for histological, biochemical and mechanical analysis to determine the effect of these agents on the structure, ECM components, and biomechanical properties. Tissues treated with SDC-based protocols still showed nuclear material, whereas tissues treated with Triton X-100 1% + ENZ +/- TRYP were completely cell free. For both decellularized tissues, an almost complete washout of glycosaminoglycans, a reduction of soluble collagen and an alteration of the surface ultrastructure was observed. Interestingly, only the elastic fibers of pericardial tissue were affected and this tissue had a decreased maximum load. This study showed that both detergents had a similar impact on the ECM. However, Triton X-100 1% + DNase/RNase (ENZ) +/- Trypsin (TRYP) is the only protocol that generated completely cell free bioscaffolds. Also, our study clearly demonstrated that the decellularization agents have more impact on pericardial tissues than on heart valve leaflets. Thus, for the purpose of tissue engineering of heart valves, it is advisable to use valvular rather than pericardial matrices.
Keywords
Acellular scaffold, EXTRACELLULAR-MATRIX, XENOGENEIC TISSUE, AORTIC VALVES, SCAFFOLD, CELLS, RECONSTRUCTION, PERICARDIUM, EXTRACTION, DISEASE, Extracellular matrix, Triton X-100, Sodium deoxycholate, Pericardium, Heart valve leaflet

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Chicago
Roosens, Annelies, Pamela Somers, Filip De Somer, Víctor Sebastián Carriel Araya, Guido Van Nooten, and Maria Cornelissen. 2016. “Impact of Detergent-based Decellularization Methods on Porcine Tissues for Heart Valve Engineering.” Annals of Biomedical Engineering 44 (9): 2827–2839.
APA
Roosens, Annelies, Somers, P., De Somer, F., Carriel Araya, V. S., Van Nooten, G., & Cornelissen, M. (2016). Impact of detergent-based decellularization methods on porcine tissues for heart valve engineering. ANNALS OF BIOMEDICAL ENGINEERING, 44(9), 2827–2839.
Vancouver
1.
Roosens A, Somers P, De Somer F, Carriel Araya VS, Van Nooten G, Cornelissen M. Impact of detergent-based decellularization methods on porcine tissues for heart valve engineering. ANNALS OF BIOMEDICAL ENGINEERING. 2016;44(9):2827–39.
MLA
Roosens, Annelies, Pamela Somers, Filip De Somer, et al. “Impact of Detergent-based Decellularization Methods on Porcine Tissues for Heart Valve Engineering.” ANNALS OF BIOMEDICAL ENGINEERING 44.9 (2016): 2827–2839. Print.
@article{8049657,
  abstract     = {To date an optimal decellularization protocol of heart valve leaflets (HVL) and pericardia (PER) with an adequate preservation of the extracellular matrix (ECM) is still lacking. This study compares a 4 day Triton X-100-based protocol with faster SDC-based protocols for the decellularization of cardiac tissues. Decellularized and non-treated HVL and PER were processed for histological, biochemical and mechanical analysis to determine the effect of these agents on the structure, ECM components, and biomechanical properties. Tissues treated with SDC-based protocols still showed nuclear material, whereas tissues treated with Triton X-100 1% + ENZ +/- TRYP were completely cell free. For both decellularized tissues, an almost complete washout of glycosaminoglycans, a reduction of soluble collagen and an alteration of the surface ultrastructure was observed. Interestingly, only the elastic fibers of pericardial tissue were affected and this tissue had a decreased maximum load. This study showed that both detergents had a similar impact on the ECM. However, Triton X-100 1% + DNase/RNase (ENZ) +/- Trypsin (TRYP) is the only protocol that generated completely cell free bioscaffolds. Also, our study clearly demonstrated that the decellularization agents have more impact on pericardial tissues than on heart valve leaflets. Thus, for the purpose of tissue engineering of heart valves, it is advisable to use valvular rather than pericardial matrices.},
  author       = {Roosens, Annelies and Somers, Pamela and De Somer, Filip and Carriel Araya, Víctor Sebastián and Van Nooten, Guido and Cornelissen, Maria},
  issn         = {0090-6964},
  journal      = {ANNALS OF BIOMEDICAL ENGINEERING},
  keywords     = {Acellular scaffold,EXTRACELLULAR-MATRIX,XENOGENEIC TISSUE,AORTIC VALVES,SCAFFOLD,CELLS,RECONSTRUCTION,PERICARDIUM,EXTRACTION,DISEASE,Extracellular matrix,Triton X-100,Sodium deoxycholate,Pericardium,Heart valve leaflet},
  language     = {eng},
  number       = {9},
  pages        = {2827--2839},
  title        = {Impact of detergent-based decellularization methods on porcine tissues for heart valve engineering},
  url          = {http://dx.doi.org/10.1007/s10439-016-1555-0},
  volume       = {44},
  year         = {2016},
}

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