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RENEB intercomparison exercises analyzing micronuclei (Cytokinesis-block Micronucleus Assay)

Julie Depuydt, Ans Baeyens UGent, Stephen Barnard, Christina Beinke, Anett Benedek, Philip Beukes, Iwona Buraczewska, Firouz Darroudi, Stefania De Sanctis, Inmaculada Dominguez, et al. (2017) INTERNATIONAL JOURNAL OF RADIATION BIOLOGY. 93(1). p.36-47
abstract
Purpose: In the framework of the ‘Realizing the European Network of Biodosimetry’ (RENEB) project, two intercomparison exercises were conducted to assess the suitability of an optimized version of the cytokinesis-block micronucleus assay, and to evaluate the capacity of a large laboratory network performing biodosimetry for radiation emergency triages. Twelve European institutions participated in the first exercise, and four non-RENEB labs were added in the second one. Materials and methods: Irradiated blood samples were shipped to participating labs, whose task was to culture these samples and provide a blind dose estimate. Micronucleus analysis was performed by automated, semi-automated and manual procedures. Results: The dose estimates provided by network laboratories were in good agreement with true administered doses. The most accurate estimates were reported for low dose points (=< 0.94 Gy). For higher dose points (>= 2.7 Gy) a larger variation in estimates was observed, though in the second exercise the number of acceptable estimates increased satisfactorily. Higher accuracy was achieved with the semi-automated method. Conclusion: The results of the two exercises performed by our network demonstrate that the micronucleus assay is a useful tool for large-scale radiation emergencies, and can be successfully implemented within a large network of laboratories.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Biodosimetry, micronucleus assay, triage, radiation accident, RENEB, RADIATION BIODOSIMETRY, BIOLOGICAL DOSIMETRY, POPULATION TRIAGE, SCORING PROCEDURE, EUROPEAN NETWORK, HIGH-THROUGHPUT, IMAGE-ANALYSIS, CYTOGENETICS, LYMPHOCYTES, VALIDATION
journal title
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
Int. J. Radiat. Biol.
volume
93
issue
1
pages
36 - 47
Web of Science type
Article
Web of Science id
000392891400006
ISSN
0955-3002
DOI
10.1080/09553002.2016.1206231
project
RENEB (Realizing the European Network in Biodosimetry)
language
English
UGent publication?
yes
classification
A1
copyright statement
Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
id
8049415
handle
http://hdl.handle.net/1854/LU-8049415
date created
2016-08-16 13:28:53
date last changed
2017-03-09 11:32:00
@article{8049415,
  abstract     = {Purpose: In the framework of the {\textquoteleft}Realizing the European Network of Biodosimetry{\textquoteright} (RENEB) project, two intercomparison exercises were conducted to assess the suitability of an optimized version of the cytokinesis-block micronucleus assay, and to evaluate the capacity of a large laboratory network performing biodosimetry for radiation emergency triages. Twelve European institutions participated in the first exercise, and four non-RENEB labs were added in the second one.
Materials and methods: Irradiated blood samples were shipped to participating labs, whose task was to culture these samples and provide a blind dose estimate. Micronucleus analysis was performed by automated, semi-automated and manual procedures.
Results: The dose estimates provided by network laboratories were in good agreement with true administered doses. The most accurate estimates were reported for low dose points (={\textlangle} 0.94 Gy). For higher dose points ({\textrangle}= 2.7 Gy) a larger variation in estimates was observed, though in the second exercise the number of acceptable estimates increased satisfactorily. Higher accuracy was achieved with the semi-automated method.
Conclusion: The results of the two exercises performed by our network demonstrate that the micronucleus assay is a useful tool for large-scale radiation emergencies, and can be successfully implemented within a large network of laboratories.},
  author       = {Depuydt, Julie and Baeyens, Ans and Barnard, Stephen and Beinke, Christina and Benedek, Anett and Beukes, Philip and Buraczewska, Iwona and Darroudi, Firouz and De Sanctis, Stefania and Dominguez, Inmaculada and Monteiro Gil, Oct{\'a}via and Hadjidekova, Valeria and Kis, Enik\unmatched{0151} and Kulka, Ulrike and Lista, Florigio and Lumniczky, Katalin and M{\textquoteright}kacher, Radhia and Moquet, Jayne and Obreja, Doina and Oestreicher, Ursula and Pajic, Jelena and Pastor, Nuria and Popova, Ljubomira and Regalbuto, Elisa and Ricoul, Michelle and Sabatier, Laure and Slabbert, Jacobus and Sommer, Sylwester and Testa, Antonella and Thierens, Hubert and Wojcik, Andrzej and Vral, Anne},
  issn         = {0955-3002},
  journal      = {INTERNATIONAL JOURNAL OF RADIATION BIOLOGY},
  keyword      = {Biodosimetry,micronucleus assay,triage,radiation accident,RENEB,RADIATION BIODOSIMETRY,BIOLOGICAL DOSIMETRY,POPULATION TRIAGE,SCORING PROCEDURE,EUROPEAN NETWORK,HIGH-THROUGHPUT,IMAGE-ANALYSIS,CYTOGENETICS,LYMPHOCYTES,VALIDATION},
  language     = {eng},
  number       = {1},
  pages        = {36--47},
  title        = {RENEB intercomparison exercises analyzing micronuclei (Cytokinesis-block Micronucleus Assay)},
  url          = {http://dx.doi.org/10.1080/09553002.2016.1206231},
  volume       = {93},
  year         = {2017},
}

Chicago
Depuydt, Julie, Ans Baeyens, Stephen Barnard, Christina Beinke, Anett Benedek, Philip Beukes, Iwona Buraczewska, et al. 2017. “RENEB Intercomparison Exercises Analyzing Micronuclei (Cytokinesis-block Micronucleus Assay).” International Journal of Radiation Biology 93 (1): 36–47.
APA
Depuydt, Julie, Baeyens, A., Barnard, S., Beinke, C., Benedek, A., Beukes, P., Buraczewska, I., et al. (2017). RENEB intercomparison exercises analyzing micronuclei (Cytokinesis-block Micronucleus Assay). INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 93(1), 36–47.
Vancouver
1.
Depuydt J, Baeyens A, Barnard S, Beinke C, Benedek A, Beukes P, et al. RENEB intercomparison exercises analyzing micronuclei (Cytokinesis-block Micronucleus Assay). INTERNATIONAL JOURNAL OF RADIATION BIOLOGY. 2017;93(1):36–47.
MLA
Depuydt, Julie, Ans Baeyens, Stephen Barnard, et al. “RENEB Intercomparison Exercises Analyzing Micronuclei (Cytokinesis-block Micronucleus Assay).” INTERNATIONAL JOURNAL OF RADIATION BIOLOGY 93.1 (2017): 36–47. Print.