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PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis

Sibu Simon UGent, Petr Skůpa, Tom Viaene, Marta Zwiewka, Ricardo Tejos Ulloa UGent, Petr Klíma, Mári Čarná, Jakub Rolčík, Riet De Rycke UGent, Ignacio Moreno, et al. (2016) NEW PHYTOLOGIST. 211(1). p.65-74
abstract
Plant development mediated by the phytohormone auxin depends on tightly controlled cellular auxin levels at its target tissue that are largely established by intercellular and intracellular auxin transport mediated by PIN auxin transporters. Among the eight members of the Arabidopsis PIN family, PIN6 is the least characterized candidate. In this study we generated functional, fluorescent protein-tagged PIN6 proteins and performed comprehensive analysis of their subcellular localization and also performed a detailed functional characterization of PIN6 and its developmental roles. The localization study of PIN6 revealed a dual localization at the plasma membrane (PM) and endoplasmic reticulum (ER). Transport and metabolic profiling assays in cultured cells and Arabidopsis strongly suggest that PIN6 mediates both auxin transport across the PM and intracellular auxin homeostasis, including the regulation of free auxin and auxin conjugates levels. As evidenced by the loss- and gain-of-function analysis, the complex function of PIN6 in auxin transport and homeostasis is required for auxin distribution during lateral and adventitious root organogenesis and for progression of these developmental processes. These results illustrate a unique position of PIN6 within the family of PIN auxin transporters and further add complexity to the developmentally crucial process of auxin transport.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
EFFLUX, PHOSPHORYLATION, PROTEINS, LATERAL ROOTS, ROOT GRAVITROPISM, PLANT DEVELOPMENT, plasma membrane (PM), PIN, lateral root, endoplasmic reticulum (ER), auxin, LOCALIZATION, TRAFFICKING, EXPRESSION, EVOLUTION
journal title
NEW PHYTOLOGIST
New Phytol.
volume
211
issue
1
pages
65 - 74
Web of Science type
Article
Web of Science id
000379212800008
JCR category
PLANT SCIENCES
JCR impact factor
7.33 (2016)
JCR rank
9/211 (2016)
JCR quartile
1 (2016)
ISSN
0028-646X
DOI
10.1111/nph.14019
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8047424
handle
http://hdl.handle.net/1854/LU-8047424
date created
2016-08-11 09:24:07
date last changed
2016-12-19 15:42:15
@article{8047424,
  abstract     = {Plant development mediated by the phytohormone auxin depends on tightly controlled cellular auxin levels at its target tissue that are largely established by intercellular and intracellular auxin transport mediated by PIN auxin transporters. Among the eight members of the Arabidopsis PIN family, PIN6 is the least characterized candidate. 
In this study we generated functional, fluorescent protein-tagged PIN6 proteins and performed comprehensive analysis of their subcellular localization and also performed a detailed functional characterization of PIN6 and its developmental roles. 
The localization study of PIN6 revealed a dual localization at the plasma membrane (PM) and endoplasmic reticulum (ER). Transport and metabolic profiling assays in cultured cells and Arabidopsis strongly suggest that PIN6 mediates both auxin transport across the PM and intracellular auxin homeostasis, including the regulation of free auxin and auxin conjugates levels. As evidenced by the loss- and gain-of-function analysis, the complex function of PIN6 in auxin transport and homeostasis is required for auxin distribution during lateral and adventitious root organogenesis and for progression of these developmental processes. 
These results illustrate a unique position of PIN6 within the family of PIN auxin transporters and further add complexity to the developmentally crucial process of auxin transport.},
  author       = {Simon, Sibu and Sk\r{u}pa, Petr and Viaene, Tom and Zwiewka, Marta and Tejos Ulloa, Ricardo and Kl{\'i}ma, Petr and \v{C}arn{\'a}, M{\'a}ri and Rol\v{c}{\'i}k, Jakub and De Rycke, Riet and Moreno, Ignacio and Dobrev, Petre I and Orellana, Ariel and Za\v{z}{\'i}malov{\'a}, E and Friml, Jiri},
  issn         = {0028-646X},
  journal      = {NEW PHYTOLOGIST},
  keyword      = {EFFLUX,PHOSPHORYLATION,PROTEINS,LATERAL ROOTS,ROOT GRAVITROPISM,PLANT DEVELOPMENT,plasma membrane (PM),PIN,lateral root,endoplasmic reticulum (ER),auxin,LOCALIZATION,TRAFFICKING,EXPRESSION,EVOLUTION},
  language     = {eng},
  number       = {1},
  pages        = {65--74},
  title        = {PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis},
  url          = {http://dx.doi.org/10.1111/nph.14019},
  volume       = {211},
  year         = {2016},
}

Chicago
Simon, Sibu, Petr Skůpa, Tom Viaene, Marta Zwiewka, Ricardo Tejos Ulloa, Petr Klíma, Mári Čarná, et al. 2016. “PIN6 Auxin Transporter at Endoplasmic Reticulum and Plasma Membrane Mediates Auxin Homeostasis and Organogenesis in Arabidopsis.” New Phytologist 211 (1): 65–74.
APA
Simon, Sibu, Skůpa, P., Viaene, T., Zwiewka, M., Tejos Ulloa, R., Klíma, P., Čarná, M., et al. (2016). PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis. NEW PHYTOLOGIST, 211(1), 65–74.
Vancouver
1.
Simon S, Skůpa P, Viaene T, Zwiewka M, Tejos Ulloa R, Klíma P, et al. PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis. NEW PHYTOLOGIST. 2016;211(1):65–74.
MLA
Simon, Sibu, Petr Skůpa, Tom Viaene, et al. “PIN6 Auxin Transporter at Endoplasmic Reticulum and Plasma Membrane Mediates Auxin Homeostasis and Organogenesis in Arabidopsis.” NEW PHYTOLOGIST 211.1 (2016): 65–74. Print.