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PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis

Sibu Simon (UGent) , Petr Skůpa, Tom Viaene (UGent) , Marta Zwiewka (UGent) , Ricardo Tejos Ulloa (UGent) , Petr Klíma, Mári Čarná, Jakub Rolčík, Riet De Rycke (UGent) , Ignacio Moreno, et al.
(2016) NEW PHYTOLOGIST. 211(1). p.65-74
Author
Organization
Abstract
Plant development mediated by the phytohormone auxin depends on tightly controlled cellular auxin levels at its target tissue that are largely established by intercellular and intracellular auxin transport mediated by PIN auxin transporters. Among the eight members of the Arabidopsis PIN family, PIN6 is the least characterized candidate. In this study we generated functional, fluorescent protein-tagged PIN6 proteins and performed comprehensive analysis of their subcellular localization and also performed a detailed functional characterization of PIN6 and its developmental roles. The localization study of PIN6 revealed a dual localization at the plasma membrane (PM) and endoplasmic reticulum (ER). Transport and metabolic profiling assays in cultured cells and Arabidopsis strongly suggest that PIN6 mediates both auxin transport across the PM and intracellular auxin homeostasis, including the regulation of free auxin and auxin conjugates levels. As evidenced by the loss- and gain-of-function analysis, the complex function of PIN6 in auxin transport and homeostasis is required for auxin distribution during lateral and adventitious root organogenesis and for progression of these developmental processes. These results illustrate a unique position of PIN6 within the family of PIN auxin transporters and further add complexity to the developmentally crucial process of auxin transport.
Keywords
EFFLUX, PHOSPHORYLATION, PROTEINS, LATERAL ROOTS, ROOT GRAVITROPISM, PLANT DEVELOPMENT, plasma membrane (PM), PIN, lateral root, endoplasmic reticulum (ER), auxin, LOCALIZATION, TRAFFICKING, EXPRESSION, EVOLUTION

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Citation

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MLA
Simon, Sibu, et al. “PIN6 Auxin Transporter at Endoplasmic Reticulum and Plasma Membrane Mediates Auxin Homeostasis and Organogenesis in Arabidopsis.” NEW PHYTOLOGIST, vol. 211, no. 1, 2016, pp. 65–74, doi:10.1111/nph.14019.
APA
Simon, S., Skůpa, P., Viaene, T., Zwiewka, M., Tejos Ulloa, R., Klíma, P., … Friml, J. (2016). PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis. NEW PHYTOLOGIST, 211(1), 65–74. https://doi.org/10.1111/nph.14019
Chicago author-date
Simon, Sibu, Petr Skůpa, Tom Viaene, Marta Zwiewka, Ricardo Tejos Ulloa, Petr Klíma, Mári Čarná, et al. 2016. “PIN6 Auxin Transporter at Endoplasmic Reticulum and Plasma Membrane Mediates Auxin Homeostasis and Organogenesis in Arabidopsis.” NEW PHYTOLOGIST 211 (1): 65–74. https://doi.org/10.1111/nph.14019.
Chicago author-date (all authors)
Simon, Sibu, Petr Skůpa, Tom Viaene, Marta Zwiewka, Ricardo Tejos Ulloa, Petr Klíma, Mári Čarná, Jakub Rolčík, Riet De Rycke, Ignacio Moreno, Petre I Dobrev, Ariel Orellana, E Zažímalová, and Jiri Friml. 2016. “PIN6 Auxin Transporter at Endoplasmic Reticulum and Plasma Membrane Mediates Auxin Homeostasis and Organogenesis in Arabidopsis.” NEW PHYTOLOGIST 211 (1): 65–74. doi:10.1111/nph.14019.
Vancouver
1.
Simon S, Skůpa P, Viaene T, Zwiewka M, Tejos Ulloa R, Klíma P, et al. PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis. NEW PHYTOLOGIST. 2016;211(1):65–74.
IEEE
[1]
S. Simon et al., “PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis,” NEW PHYTOLOGIST, vol. 211, no. 1, pp. 65–74, 2016.
@article{8047424,
  abstract     = {{Plant development mediated by the phytohormone auxin depends on tightly controlled cellular auxin levels at its target tissue that are largely established by intercellular and intracellular auxin transport mediated by PIN auxin transporters. Among the eight members of the Arabidopsis PIN family, PIN6 is the least characterized candidate. 
In this study we generated functional, fluorescent protein-tagged PIN6 proteins and performed comprehensive analysis of their subcellular localization and also performed a detailed functional characterization of PIN6 and its developmental roles. 
The localization study of PIN6 revealed a dual localization at the plasma membrane (PM) and endoplasmic reticulum (ER). Transport and metabolic profiling assays in cultured cells and Arabidopsis strongly suggest that PIN6 mediates both auxin transport across the PM and intracellular auxin homeostasis, including the regulation of free auxin and auxin conjugates levels. As evidenced by the loss- and gain-of-function analysis, the complex function of PIN6 in auxin transport and homeostasis is required for auxin distribution during lateral and adventitious root organogenesis and for progression of these developmental processes. 
These results illustrate a unique position of PIN6 within the family of PIN auxin transporters and further add complexity to the developmentally crucial process of auxin transport.}},
  author       = {{Simon, Sibu and Skůpa, Petr and Viaene, Tom and Zwiewka, Marta and Tejos Ulloa, Ricardo and Klíma, Petr and Čarná, Mári and Rolčík, Jakub and De Rycke, Riet and Moreno, Ignacio and Dobrev, Petre I and Orellana, Ariel and Zažímalová, E and Friml, Jiri}},
  issn         = {{0028-646X}},
  journal      = {{NEW PHYTOLOGIST}},
  keywords     = {{EFFLUX,PHOSPHORYLATION,PROTEINS,LATERAL ROOTS,ROOT GRAVITROPISM,PLANT DEVELOPMENT,plasma membrane (PM),PIN,lateral root,endoplasmic reticulum (ER),auxin,LOCALIZATION,TRAFFICKING,EXPRESSION,EVOLUTION}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{65--74}},
  title        = {{PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis}},
  url          = {{http://doi.org/10.1111/nph.14019}},
  volume       = {{211}},
  year         = {{2016}},
}
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