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Mitochondrial uncouplers inhibit clathrin-mediated endocytosis largely through cytoplasmic acidification

Wim Dejonghe, Sabine Kuenen, Evelien Mylle UGent, Mina Vasileva, Olivier Keech, Corrado Viotti, Jef Swerts, Matyas Fendrych, Fausto Andres Ortiz Morea, Kiril Mishev, et al. (2016) NATURE COMMUNICATIONS. 7.
abstract
ATP production requires the establishment of an electrochemical proton gradient across the inner mitochondrial membrane. Mitochondrial uncouplers dissipate this proton gradient and disrupt numerous cellular processes, including vesicular trafficking, mainly through energy depletion. Here we show that Endosidin9 (ES9), a novel mitochondrial uncoupler, is a potent inhibitor of clathrin-mediated endocytosis (CME) in different systems and that ES9 induces inhibition of CME not because of its effect on cellular ATP, but rather due to its protonophore activity that leads to cytoplasm acidification. We show that the known tyrosine kinase inhibitor tyrphostinA23, which is routinely used to block CME, displays similar properties, thus questioning its use as a specific inhibitor of cargo recognition by the AP-2 adaptor complex via tyrosine motif-based endocytosis signals. Furthermore, we show that cytoplasm acidification dramatically affects the dynamics and recruitment of clathrin and associated adaptors, and leads to reduction of phosphatidylinositol 4,5-biphosphate from the plasma membrane.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ADAPTER COMPLEX, ARABIDOPSIS-THALIANA, OXIDATIVE-PHOSPHORYLATION, ALPHA-ADAPTIN, COATED PITS, ANTIMYCIN-A, 5-BISPHOSPHATE, PHOSPHATIDYLINOSITOL 4, PLASMA-MEMBRANE, ATPASE ACTIVITY, TERMINAL DOMAIN
journal title
NATURE COMMUNICATIONS
Nat. Commun.
volume
7
article number
11710
pages
12 pages
Web of Science type
Article
Web of Science id
000377899800001
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
12.124 (2016)
JCR rank
3/64 (2016)
JCR quartile
1 (2016)
ISSN
2041-1723
DOI
10.1038/ncomms11710
language
English
UGent publication?
yes
classification
A1
copyright statement
Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
id
8036372
handle
http://hdl.handle.net/1854/LU-8036372
date created
2016-07-14 09:19:55
date last changed
2017-05-04 13:45:52
@article{8036372,
  abstract     = {ATP production requires the establishment of an electrochemical proton gradient across the inner mitochondrial membrane. Mitochondrial uncouplers dissipate this proton gradient and disrupt numerous cellular processes, including vesicular trafficking, mainly through energy depletion. Here we show that Endosidin9 (ES9), a novel mitochondrial uncoupler, is a potent inhibitor of clathrin-mediated endocytosis (CME) in different systems and that ES9 induces inhibition of CME not because of its effect on cellular ATP, but rather due to its protonophore activity that leads to cytoplasm acidification. We show that the known tyrosine kinase inhibitor tyrphostinA23, which is routinely used to block CME, displays similar properties, thus questioning its use as a specific inhibitor of cargo recognition by the AP-2 adaptor complex via tyrosine motif-based endocytosis signals. Furthermore, we show that cytoplasm acidification dramatically affects the dynamics and recruitment of clathrin and associated adaptors, and leads to reduction of phosphatidylinositol 4,5-biphosphate from the plasma membrane.},
  articleno    = {11710},
  author       = {Dejonghe, Wim and Kuenen, Sabine and Mylle, Evelien and Vasileva, Mina and Keech, Olivier and Viotti, Corrado and Swerts, Jef and Fendrych, Matyas and Ortiz Morea, Fausto Andres and Mishev, Kiril and Delang, Simon and Scholl, Stefan and Zarza, Xavier and Heilmann, Mareike and Kourelis, Jiorgos and Kasprowicz, Jaroslaw and Nguyen, Long and Drozdzecki, Andrzej and Vanhoutte, Isabelle and Szatmari, Anna Maria and Majda, Mateusz and Baisa, Gary and Bednarek, Sebastian York and Robert, Stephanie and Audenaert, Dominique and Testerink, Christa and Munnik, Teun and Van Damme, Dani{\"e}l and Heilmann, Ingo and Schumacher, Karin and Winne, Johan and Friml, Jiri and Verstreken, Patrik and Russinova, Eugenia},
  issn         = {2041-1723},
  journal      = {NATURE COMMUNICATIONS},
  keyword      = {ADAPTER COMPLEX,ARABIDOPSIS-THALIANA,OXIDATIVE-PHOSPHORYLATION,ALPHA-ADAPTIN,COATED PITS,ANTIMYCIN-A,5-BISPHOSPHATE,PHOSPHATIDYLINOSITOL 4,PLASMA-MEMBRANE,ATPASE ACTIVITY,TERMINAL DOMAIN},
  language     = {eng},
  pages        = {12},
  title        = {Mitochondrial uncouplers inhibit clathrin-mediated endocytosis largely through cytoplasmic acidification},
  url          = {http://dx.doi.org/10.1038/ncomms11710},
  volume       = {7},
  year         = {2016},
}

Chicago
Dejonghe, Wim, Sabine Kuenen, Evelien Mylle, Mina Vasileva, Olivier Keech, Corrado Viotti, Jef Swerts, et al. 2016. “Mitochondrial Uncouplers Inhibit Clathrin-mediated Endocytosis Largely Through Cytoplasmic Acidification.” Nature Communications 7.
APA
Dejonghe, Wim, Kuenen, S., Mylle, E., Vasileva, M., Keech, O., Viotti, C., Swerts, J., et al. (2016). Mitochondrial uncouplers inhibit clathrin-mediated endocytosis largely through cytoplasmic acidification. NATURE COMMUNICATIONS, 7.
Vancouver
1.
Dejonghe W, Kuenen S, Mylle E, Vasileva M, Keech O, Viotti C, et al. Mitochondrial uncouplers inhibit clathrin-mediated endocytosis largely through cytoplasmic acidification. NATURE COMMUNICATIONS. 2016;7.
MLA
Dejonghe, Wim, Sabine Kuenen, Evelien Mylle, et al. “Mitochondrial Uncouplers Inhibit Clathrin-mediated Endocytosis Largely Through Cytoplasmic Acidification.” NATURE COMMUNICATIONS 7 (2016): n. pag. Print.