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Enhanced release of IgE-dependent early phase mediators from nasal polyp tissue

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Background: The mast cell is a crucial effector cell in allergic rhinitis and other inflammatory diseases. During the acute allergic reaction preformed mediators such as histamine, but also de novo produced mediators such as leukotrienes (LTC4/D-4/E-4) and prostaglandins (PGD(2)) are released. Mast cells represent targets for therapeutic intervention, and thus a human ex-vivo model to stimulate mast cells taken from mucosal sites would be instrumental for drug intervention studies. We have aimed to activate mast cells within ex-vivo human nasal tissue by IgE/anti-IgE specific (epsilon chain specific) stimulations and in this respect to test the usability of nasal polyps versus inferior turbinates Methods: Biopsy samples were collected from patients with nasal polyps and inferior turbinates from patients who underwent sinus or septal surgery. Tissue fragments were primed with IgE 1 mu g/ml for 60 minutes and then stimulated for 30 minutes with tissue culture medium (negative control), anti-IgE 10 mu g/ml, anti-IgE 30 mu g/ml and ionomycin 10 mu M (positive control). Histamine, leukotrienes and PGD2 were measured in supernatants. To help provide an understanding of the extent of the response, the number of tryptase and Fc epsilon RI alpha positive cells was evaluated by means of immunohistochemistry and the Fc epsilon RI alpha-chain was measured by means of quantitative PCR in the nasal polyp and inferior turbinate tissues. Finally, the correlation between IgE concentrations in the nasal tissue and the release of mediators was analysed. Results: Stimulations with anti-IgE on IgE-primed nasal tissue fragments lead to a concentration-dependent release of histamine, leukotrienes and PGD(2). The release of these early phase mediators was significantly higher in nasal polyps compared to inferior turbinates, although tryptase, Fc epsilon RI alpha positive cells and Fc epsilon RI alpha-chain transcripts were equally present in both groups. No correlation was found between baseline concentrations of IgE, and the release of histamine, LTC4/LTD4/LTE4 and PGD2 after stimulation. Conclusion: This human nasal challenge model mimics the allergic early phase reaction. The release of histamine, cys-leukotrienes and PGD(2) was significantly higher in nasal polyps versus inferior turbinates, however, this observation could not be explained by differences in mast cell or Fc epsilon RI+ cell numbers.

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Chicago
Patou, Joke, Gabriële Holtappels, Karen Affleck, Philippe Gevaert, Claudina Perez Novo, Paul Van Cauwenberge, and Claus Bachert. 2009. “Enhanced Release of IgE-dependent Early Phase Mediators from Nasal Polyp Tissue.” Journal of Inflammation-london 6 (AR11): AR11.
APA
Patou, J., Holtappels, G., Affleck, K., Gevaert, P., Perez Novo, C., Van Cauwenberge, P., & Bachert, C. (2009). Enhanced release of IgE-dependent early phase mediators from nasal polyp tissue. JOURNAL OF INFLAMMATION-LONDON, 6(AR11), AR11.
Vancouver
1.
Patou J, Holtappels G, Affleck K, Gevaert P, Perez Novo C, Van Cauwenberge P, et al. Enhanced release of IgE-dependent early phase mediators from nasal polyp tissue. JOURNAL OF INFLAMMATION-LONDON. 2009;6(AR11):AR11.
MLA
Patou, Joke, Gabriële Holtappels, Karen Affleck, et al. “Enhanced Release of IgE-dependent Early Phase Mediators from Nasal Polyp Tissue.” JOURNAL OF INFLAMMATION-LONDON 6.AR11 (2009): AR11. Print.
@article{803389,
  abstract     = {Background: The mast cell is a crucial effector cell in allergic rhinitis and other inflammatory diseases. During the acute allergic reaction preformed mediators such as histamine, but also de novo produced mediators such as leukotrienes (LTC4/D-4/E-4) and prostaglandins (PGD(2)) are released. Mast cells represent targets for therapeutic intervention, and thus a human ex-vivo model to stimulate mast cells taken from mucosal sites would be instrumental for drug intervention studies. We have aimed to activate mast cells within ex-vivo human nasal tissue by IgE/anti-IgE specific (epsilon chain specific) stimulations and in this respect to test the usability of nasal polyps versus inferior turbinates

Methods: Biopsy samples were collected from patients with nasal polyps and inferior turbinates from patients who underwent sinus or septal surgery. Tissue fragments were primed with IgE 1 mu g/ml for 60 minutes and then stimulated for 30 minutes with tissue culture medium (negative control), anti-IgE 10 mu g/ml, anti-IgE 30 mu g/ml and ionomycin 10 mu M (positive control). Histamine, leukotrienes and PGD2 were measured in supernatants. To help provide an understanding of the extent of the response, the number of tryptase and Fc epsilon RI alpha positive cells was evaluated by means of immunohistochemistry and the Fc epsilon RI alpha-chain was measured by means of quantitative PCR in the nasal polyp and inferior turbinate tissues. Finally, the correlation between IgE concentrations in the nasal tissue and the release of mediators was analysed.

Results: Stimulations with anti-IgE on IgE-primed nasal tissue fragments lead to a concentration-dependent release of histamine, leukotrienes and PGD(2). The release of these early phase mediators was significantly higher in nasal polyps compared to inferior turbinates, although tryptase, Fc epsilon RI alpha positive cells and Fc epsilon RI alpha-chain transcripts were equally present in both groups. No correlation was found between baseline concentrations of IgE, and the release of histamine, LTC4/LTD4/LTE4 and PGD2 after stimulation.

Conclusion: This human nasal challenge model mimics the allergic early phase reaction. The release of histamine, cys-leukotrienes and PGD(2) was significantly higher in nasal polyps versus inferior turbinates, however, this observation could not be explained by differences in mast cell or Fc epsilon RI+ cell numbers.},
  author       = {Patou, Joke and Holtappels, Gabri{\"e}le and Affleck, Karen and Gevaert, Philippe and Perez Novo, Claudina and Van Cauwenberge, Paul and Bachert, Claus},
  issn         = {1476-9255},
  journal      = {JOURNAL OF INFLAMMATION-LONDON},
  language     = {eng},
  number       = {AR11},
  title        = {Enhanced release of IgE-dependent early phase mediators from nasal polyp tissue},
  url          = {http://dx.doi.org/10.1186/1476-9255-6-11},
  volume       = {6},
  year         = {2009},
}

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