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Fermentable soluble fibres spare amino acids in healthy dogs fed a low-protein diet

Wendy Wambacq UGent, Galyna Rybachuk, Isabelle Jeusette, Kristel Rochus, Brigitte Wuyts, Veerle Fievez UGent, Patrick Nguyen and Myriam Hesta UGent (2016) BMC VETERINARY RESEARCH. 12.
abstract
Background: Research in cats has shown that increased fermentation-derived propionic acid and its metabolites can be used as alternative substrates for gluconeogenesis, thus sparing amino acids for other purposes. This amino acid sparing effect could be of particular interest in patients with kidney or liver disease, where this could reduce the kidneys'/liver's burden of N-waste removal. Since dogs are known to have a different metabolism than the obligatory carnivorous cat, the main objective of this study was to assess the possibility of altering amino acid metabolism through intestinal fermentation in healthy dogs. This was studied by supplementing a low-protein diet with fermentable fibres, hereby providing an initial model for future studies in dogs suffering from renal/liver disease. Results: Eight healthy dogs were randomly assigned to one of two treatment groups: sugar beet pulp and guar gum mix (SF: soluble fibre, estimated to mainly stimulate propionic acid production) or cellulose (IF: insoluble fibre). Treatments were incorporated into a low-protein (17 %) extruded dry diet in amounts to obtain similar total dietary fibre (TDF) contents for both diets (9.4 % and 8.2 % for the SF and IF diet, respectively) and were tested in a 4-week crossover feeding trial. Apparent faecal nitrogen digestibility and post-prandial fermentation metabolites in faeces and plasma were evaluated. Dogs fed the SF diet showed significantly higher faecal excretion of acetic and propionic acid, resulting in a higher total SCFA excretion compared to IF. SF affected the three to six-hour postprandial plasma acylcarnitine profile by significantly increasing AUC of acetyl-, propionyl-, butyryl- + isobutyryl-, 3-OH-butyryl-, 3-OH-isovaleryl- and malonyl-L-carnitine. Moreover, the amino acid plasma profile at that time was modified as leucine + isoleucine concentrations were significantly increased by SF, and a similar trend for phenylalanine and tyrosine's AUC was found. Conclusion: These results indicate that guar gum and sugar beet pulp supplementation diminishes postprandial use of amino acids favoring instead the use of short-chain fatty acids as substrate for the tricarboxylic acid (TCA) cycle. Further research is warranted to investigate the amino acid sparing effect of fermentable fibres in dogs with kidney/liver disease.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Acylcarnitine, Canine renal diet, Apparent protein digestibility, Dietary fibre, Guar gum, MEAN RETENTION TIME, IN-VITRO FERMENTATION, METABOLIZABLE ENERGY, NUTRIENT INTAKE, INTESTINAL FERMENTATION, FECAL INOCULUM, DOMESTIC CATS, TRANSIT-TIME, BEET PULP, DIGESTIBILITY
journal title
BMC VETERINARY RESEARCH
BMC Vet. Res.
volume
12
article number
130
pages
10 pages
Web of Science type
Article
Web of Science id
000378997500001
JCR category
VETERINARY SCIENCES
JCR impact factor
1.75 (2016)
JCR rank
24/136 (2016)
JCR quartile
1 (2016)
ISSN
1746-6148
DOI
10.1186/s12917-016-0752-2
language
English
UGent publication?
yes
classification
A1
additional info
the first two authors are equal contributors
copyright statement
I have retained and own the full copyright for this publication
id
8033482
handle
http://hdl.handle.net/1854/LU-8033482
date created
2016-07-10 09:37:29
date last changed
2016-12-21 15:41:27
@article{8033482,
  abstract     = {Background: Research in cats has shown that increased fermentation-derived propionic acid and its metabolites can be used as alternative substrates for gluconeogenesis, thus sparing amino acids for other purposes. This amino acid sparing effect could be of particular interest in patients with kidney or liver disease, where this could reduce the kidneys'/liver's burden of N-waste removal. Since dogs are known to have a different metabolism than the obligatory carnivorous cat, the main objective of this study was to assess the possibility of altering amino acid metabolism through intestinal fermentation in healthy dogs. This was studied by supplementing a low-protein diet with fermentable fibres, hereby providing an initial model for future studies in dogs suffering from renal/liver disease. 
Results: Eight healthy dogs were randomly assigned to one of two treatment groups: sugar beet pulp and guar gum mix (SF: soluble fibre, estimated to mainly stimulate propionic acid production) or cellulose (IF: insoluble fibre). Treatments were incorporated into a low-protein (17 \%) extruded dry diet in amounts to obtain similar total dietary fibre (TDF) contents for both diets (9.4 \% and 8.2 \% for the SF and IF diet, respectively) and were tested in a 4-week crossover feeding trial. Apparent faecal nitrogen digestibility and post-prandial fermentation metabolites in faeces and plasma were evaluated. Dogs fed the SF diet showed significantly higher faecal excretion of acetic and propionic acid, resulting in a higher total SCFA excretion compared to IF. SF affected the three to six-hour postprandial plasma acylcarnitine profile by significantly increasing AUC of acetyl-, propionyl-, butyryl- + isobutyryl-, 3-OH-butyryl-, 3-OH-isovaleryl- and malonyl-L-carnitine. Moreover, the amino acid plasma profile at that time was modified as leucine + isoleucine concentrations were significantly increased by SF, and a similar trend for phenylalanine and tyrosine's AUC was found. 
Conclusion: These results indicate that guar gum and sugar beet pulp supplementation diminishes postprandial use of amino acids favoring instead the use of short-chain fatty acids as substrate for the tricarboxylic acid (TCA) cycle. Further research is warranted to investigate the amino acid sparing effect of fermentable fibres in dogs with kidney/liver disease.},
  articleno    = {130},
  author       = {Wambacq, Wendy and Rybachuk, Galyna and Jeusette, Isabelle and Rochus, Kristel and Wuyts, Brigitte and Fievez, Veerle and Nguyen, Patrick and Hesta, Myriam},
  issn         = {1746-6148},
  journal      = {BMC VETERINARY RESEARCH},
  keyword      = {Acylcarnitine,Canine renal diet,Apparent protein digestibility,Dietary fibre,Guar gum,MEAN RETENTION TIME,IN-VITRO FERMENTATION,METABOLIZABLE ENERGY,NUTRIENT INTAKE,INTESTINAL FERMENTATION,FECAL INOCULUM,DOMESTIC CATS,TRANSIT-TIME,BEET PULP,DIGESTIBILITY},
  language     = {eng},
  pages        = {10},
  title        = {Fermentable soluble fibres spare amino acids in healthy dogs fed a low-protein diet},
  url          = {http://dx.doi.org/10.1186/s12917-016-0752-2},
  volume       = {12},
  year         = {2016},
}

Chicago
Wambacq, Wendy, Galyna Rybachuk, Isabelle Jeusette, Kristel Rochus, Brigitte Wuyts, Veerle Fievez, Patrick Nguyen, and Myriam Hesta. 2016. “Fermentable Soluble Fibres Spare Amino Acids in Healthy Dogs Fed a Low-protein Diet.” Bmc Veterinary Research 12.
APA
Wambacq, W., Rybachuk, G., Jeusette, I., Rochus, K., Wuyts, B., Fievez, V., Nguyen, P., et al. (2016). Fermentable soluble fibres spare amino acids in healthy dogs fed a low-protein diet. BMC VETERINARY RESEARCH, 12.
Vancouver
1.
Wambacq W, Rybachuk G, Jeusette I, Rochus K, Wuyts B, Fievez V, et al. Fermentable soluble fibres spare amino acids in healthy dogs fed a low-protein diet. BMC VETERINARY RESEARCH. 2016;12.
MLA
Wambacq, Wendy, Galyna Rybachuk, Isabelle Jeusette, et al. “Fermentable Soluble Fibres Spare Amino Acids in Healthy Dogs Fed a Low-protein Diet.” BMC VETERINARY RESEARCH 12 (2016): n. pag. Print.