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The effect of topical amphotericin B on inflammatory markers in patients with chronic rhinosinusitis: a multicenter randomized controlled study

(2009) LARYNGOSCOPE. 119(2). p.401-408
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Abstract
Background: It has been suggested that an exaggerated immune response to fungi is crucial in the pathogenesis of chronic rhinosinusitis (CRS). Based on this rationale, the use of topical antifungals (amphotericin B) has been advocated. Studies on its clinical effectiveness are, however, contradictory. Objectives: To examine the effect of nasal antifungal treatment on secreted mediators in samples of nasal lavage fluid from patients with CRS with or without nasal polyps (NP). Methods: Part two of a prospective double-blind, placebo-controlled multicenter clinical trial investigating the effect of 13 weeks of treatment with amphotericin B or placebo on the levels of pro-inflammatory cytokines, chemokines and growth factors (i.e., IL-1 beta, IL-1RA, IL-2, IL-2R, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40/p70 subunits), IL-13, IL-15, IL-17, TNF-alpha, IFN-alpha, IFN-gamma, G-CSF, GM-CSF MIP-1 alpha, MIP-1 beta, IP-10, MIG, eotaxin, RANTES, MCP-1, MCP-2, MCP-3, VEGF, EGF, FGF-basic, HGF, Gro-alpha) and albumin via a fluorescent enzyme immunoassay in nasal lavage specimens of CRS patients with or without NP. Results: Topical amphotericin B had no significant effect on the level of any of the tested proinflammatory cytokines, chemokines, and growth factors in CRS nasal lavage samples. Treatment with placebo, however, increased the level of MIP-1 alpha and MIP-1 beta, which are mediators involved in wound healing. Conclusions: Topical amphotericin B has no significant effect on activation markers of nasal inflammatory cells in chronic rhinosinusitis with or without nasal polyps.
Keywords
TRIAL, LAVAGE, IRRIGATION, SINUSITIS, INFECTION, CYTOKINES, EPITHELIAL-CELLS, THERAPY, NASAL POLYPS, ANTIFUNGAL TREATMENT, chemokine CCL3, chemokine CCL4, intracellular signalling peptides and proteins, nasal lavage fluid, fungi, cytokines, chemokines, chronic disease, rhinosinusitis, nasal polyps, intranasal administration, prospective study, double-blind method, human, amphotericin B, Randomized controlled trial

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Citation

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Chicago
Ebbens, Fenna A, Christos Georgalas, Silvia Luiten, Cornelis M van Drunen, Lydia Badia, Glenis K Scadding, Peter W Hellings, et al. 2009. “The Effect of Topical Amphotericin B on Inflammatory Markers in Patients with Chronic Rhinosinusitis: a Multicenter Randomized Controlled Study.” Laryngoscope 119 (2): 401–408.
APA
Ebbens, F. A., Georgalas, C., Luiten, S., van Drunen, C. M., Badia, L., Scadding, G. K., Hellings, P. W., et al. (2009). The effect of topical amphotericin B on inflammatory markers in patients with chronic rhinosinusitis: a multicenter randomized controlled study. LARYNGOSCOPE, 119(2), 401–408.
Vancouver
1.
Ebbens FA, Georgalas C, Luiten S, van Drunen CM, Badia L, Scadding GK, et al. The effect of topical amphotericin B on inflammatory markers in patients with chronic rhinosinusitis: a multicenter randomized controlled study. LARYNGOSCOPE. 2009;119(2):401–8.
MLA
Ebbens, Fenna A, Christos Georgalas, Silvia Luiten, et al. “The Effect of Topical Amphotericin B on Inflammatory Markers in Patients with Chronic Rhinosinusitis: a Multicenter Randomized Controlled Study.” LARYNGOSCOPE 119.2 (2009): 401–408. Print.
@article{803292,
  abstract     = {Background: It has been suggested that an exaggerated immune response to fungi is crucial in the pathogenesis of chronic rhinosinusitis (CRS). Based on this rationale, the use of topical antifungals (amphotericin B) has been advocated. Studies on its clinical effectiveness are, however, contradictory. 
Objectives: To examine the effect of nasal antifungal treatment on secreted mediators in samples of nasal lavage fluid from patients with CRS with or without nasal polyps (NP). 
Methods: Part two of a prospective double-blind, placebo-controlled multicenter clinical trial investigating the effect of 13 weeks of treatment with amphotericin B or placebo on the levels of pro-inflammatory cytokines, chemokines and growth factors (i.e., IL-1 beta, IL-1RA, IL-2, IL-2R, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40/p70 subunits), IL-13, IL-15, IL-17, TNF-alpha, IFN-alpha, IFN-gamma, G-CSF, GM-CSF MIP-1 alpha, MIP-1 beta, IP-10, MIG, eotaxin, RANTES, MCP-1, MCP-2, MCP-3, VEGF, EGF, FGF-basic, HGF, Gro-alpha) and albumin via a fluorescent enzyme immunoassay in nasal lavage specimens of CRS patients with or without NP. 
Results: Topical amphotericin B had no significant effect on the level of any of the tested proinflammatory cytokines, chemokines, and growth factors in CRS nasal lavage samples. Treatment with placebo, however, increased the level of MIP-1 alpha and MIP-1 beta, which are mediators involved in wound healing. 
Conclusions: Topical amphotericin B has no significant effect on activation markers of nasal inflammatory cells in chronic rhinosinusitis with or without nasal polyps.},
  author       = {Ebbens, Fenna A and Georgalas, Christos and Luiten, Silvia and van Drunen, Cornelis M and Badia, Lydia and Scadding, Glenis K and Hellings, Peter W and Jorissen, Mark and Mullol, Joaquim and Cardesin, Alda and Bachert, Claus and Van Zele, Thibaut and Lund, Valerie J and Fokkens, WJ},
  issn         = {0023-852X},
  journal      = {LARYNGOSCOPE},
  language     = {eng},
  number       = {2},
  pages        = {401--408},
  title        = {The effect of topical amphotericin B on inflammatory markers in patients with chronic rhinosinusitis: a multicenter randomized controlled study},
  url          = {http://dx.doi.org/10.1002/lary.20064},
  volume       = {119},
  year         = {2009},
}

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