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Th2 biased upper airway inflammation is associated with an impaired response to viral infection with Herpes simplex virus 1

(2016) RHINOLOGY. 54(2). p.141-149
Author
Organization
Abstract
Background: We aimed to elucidate possible differences in antiviral defense in chronic rhinosinusitis with nasal polyps (CRSwNP) mucosal tissue compared to healthy mucosal tissue (HMT) upon herpes simplex virus 1 (HSV1) exposure. Methodology: HMT and CRSwNP samples were infected with HSV1. We visualized the virus location by immunofluorescence and monitored invasion by a score. The mediators Interferon (IFN)-alpha, IFN-beta, IFN-lambda, IFN-gamma, Interleukin (IL)-6, IL-1 beta, Tumor necrosis factor (TNF)-alpha, IL-17, IL-5, IL-10 were measured in culture supernatants at baseline and at 24h, 48h and 72h after virus incubation. Results: CRSwNP mucosal tissue showed a significant deficit in IFN-gamma and IL-17 release within 24 to 72 hours after infection in comparison to HMT, at the same time releasing significantly more pro-inflammatory cytokines including IL-1 beta and TNF-alpha. These findings were associated with significantly higher viral invasion scores at 48 and 72 h in CRSwNP mucosa compared to those for the HMT. Conclusions: We demonstrate for the first time in a human ex-vivo mucosal model that the inadequate response of CRSwNP may be associated with a deeper intrusion of viruses into the mucosal tissue, and may contribute to more and longer symptoms upon acute infection, but also to the persistence of inflammation in CRSwNP tissue.
Keywords
nasal polyps, interferon-gamma, herpes simplex virus 1 infection, human ex vivo mucosa model, STAPHYLOCOCCUS-AUREUS ENTEROTOXINS, EXPERIMENTAL RHINOVIRUS INFECTION, CHRONIC RHINOSINUSITIS, NASAL POLYPS, ASTHMA EXACERBATIONS, INTERFERON-LAMBDA, SINUS DISEASE, INTERLEUKIN-10, TYPE-1, GAMMA

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Citation

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MLA
Lan, Feng et al. “Th2 Biased Upper Airway Inflammation Is Associated with an Impaired Response to Viral Infection with Herpes Simplex Virus 1.” RHINOLOGY 54.2 (2016): 141–149. Print.
APA
Lan, F., Wane, X. D., Nauwynck, H., Holtappels, G., Zhang, L., Johnston, S. L., Papadopoulos, N. G., et al. (2016). Th2 biased upper airway inflammation is associated with an impaired response to viral infection with Herpes simplex virus 1. RHINOLOGY, 54(2), 141–149.
Chicago author-date
Lan, Feng, Xiang Dong Wane, Hans Nauwynck, Gabriële Holtappels, Luo Zhang, Sebastian L Johnston, Nikolaos G Papadopoulos, Claus Bachert, and Nan Zhang. 2016. “Th2 Biased Upper Airway Inflammation Is Associated with an Impaired Response to Viral Infection with Herpes Simplex Virus 1.” Rhinology 54 (2): 141–149.
Chicago author-date (all authors)
Lan, Feng, Xiang Dong Wane, Hans Nauwynck, Gabriële Holtappels, Luo Zhang, Sebastian L Johnston, Nikolaos G Papadopoulos, Claus Bachert, and Nan Zhang. 2016. “Th2 Biased Upper Airway Inflammation Is Associated with an Impaired Response to Viral Infection with Herpes Simplex Virus 1.” Rhinology 54 (2): 141–149.
Vancouver
1.
Lan F, Wane XD, Nauwynck H, Holtappels G, Zhang L, Johnston SL, et al. Th2 biased upper airway inflammation is associated with an impaired response to viral infection with Herpes simplex virus 1. RHINOLOGY. 2016;54(2):141–9.
IEEE
[1]
F. Lan et al., “Th2 biased upper airway inflammation is associated with an impaired response to viral infection with Herpes simplex virus 1,” RHINOLOGY, vol. 54, no. 2, pp. 141–149, 2016.
@article{8031645,
  abstract     = {Background: We aimed to elucidate possible differences in antiviral defense in chronic rhinosinusitis with nasal polyps (CRSwNP) mucosal tissue compared to healthy mucosal tissue (HMT) upon herpes simplex virus 1 (HSV1) exposure. 
Methodology: HMT and CRSwNP samples were infected with HSV1. We visualized the virus location by immunofluorescence and monitored invasion by a score. The mediators Interferon (IFN)-alpha, IFN-beta, IFN-lambda, IFN-gamma, Interleukin (IL)-6, IL-1 beta, Tumor necrosis factor (TNF)-alpha, IL-17, IL-5, IL-10 were measured in culture supernatants at baseline and at 24h, 48h and 72h after virus incubation. 
Results: CRSwNP mucosal tissue showed a significant deficit in IFN-gamma and IL-17 release within 24 to 72 hours after infection in comparison to HMT, at the same time releasing significantly more pro-inflammatory cytokines including IL-1 beta and TNF-alpha. These findings were associated with significantly higher viral invasion scores at 48 and 72 h in CRSwNP mucosa compared to those for the HMT. 
Conclusions: We demonstrate for the first time in a human ex-vivo mucosal model that the inadequate response of CRSwNP may be associated with a deeper intrusion of viruses into the mucosal tissue, and may contribute to more and longer symptoms upon acute infection, but also to the persistence of inflammation in CRSwNP tissue.},
  author       = {Lan, Feng and Wane, Xiang Dong and Nauwynck, Hans and Holtappels, Gabriële and Zhang, Luo and Johnston, Sebastian L and Papadopoulos, Nikolaos G and Bachert, Claus and Zhang, Nan},
  issn         = {0300-0729},
  journal      = {RHINOLOGY},
  keywords     = {nasal polyps,interferon-gamma,herpes simplex virus 1 infection,human ex vivo mucosa model,STAPHYLOCOCCUS-AUREUS ENTEROTOXINS,EXPERIMENTAL RHINOVIRUS INFECTION,CHRONIC RHINOSINUSITIS,NASAL POLYPS,ASTHMA EXACERBATIONS,INTERFERON-LAMBDA,SINUS DISEASE,INTERLEUKIN-10,TYPE-1,GAMMA},
  language     = {eng},
  number       = {2},
  pages        = {141--149},
  title        = {Th2 biased upper airway inflammation is associated with an impaired response to viral infection with Herpes simplex virus 1},
  url          = {http://dx.doi.org/10.4193/Rhino15.213},
  volume       = {54},
  year         = {2016},
}

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