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Duality of β-glucan microparticles: antigen carrier and immunostimulants

Kim Baert, Bruno De Geest UGent, Henri De Greve, Eric Cox UGent and Bert Devriendt UGent (2016) INTERNATIONAL JOURNAL OF NANOMEDICINE. 11. p.2463-2469
abstract
Designing efficient recombinant mucosal vaccines against enteric diseases is still a major challenge. Mucosal delivery of recombinant vaccines requires encapsulation in potent immunostimulatory particles to induce an efficient immune response. This paper evaluates the capacity of beta-glucan microparticles (GPs) as antigen vehicles and characterizes their immune-stimulatory effects. The relevant infectious antigen FedF was chosen to be loaded inside the microparticles. The incorporation of FedF inside the particles was highly efficient (roughly 85%) and occurred without antigen degradation. In addition, these GPs have immunostimulatory effects as well, demonstrated by the strong reactive oxygen species (ROS) production by porcine neutrophils upon their recognition. Although antigen-loaded GPs still induce ROS production, antigen loading decreases this production by neutrophils for reasons yet unknown. However, these antigen-loaded GPs are still able to bind their specific beta-glucan receptor, demonstrated by blocking complement receptor 3, which is the major beta-glucan receptor on porcine neutrophils. The dual character of these particles is confirmed by a T-cell proliferation assay. FedF-loaded particles induce a significantly higher FedF-specific T-cell proliferation than soluble FedF. Taken together, these results show that GPs are efficient antigen carriers with immune-stimulatory properties.
Please use this url to cite or link to this publication:
author
organization
alternative title
Duality of beta-glucan microparticles : antigen carrier and immunostimulants
year
type
journalArticle (original)
publication status
published
subject
keyword
antigen delivery vehicle, β-glucan microparticles, FedF, immunostimulants, FIMBRIAL ADHESIN FEDF, DELIVERY, VACCINE, BINDING, CELLS, COLI, IMMUNIZATION, COMPLEMENT, PIGLETS, SYSTEM
journal title
INTERNATIONAL JOURNAL OF NANOMEDICINE
Int. J. Nanomed.
volume
11
pages
2463 - 2469
Web of Science type
Article
Web of Science id
000377085000001
JCR category
PHARMACOLOGY & PHARMACY
JCR impact factor
4.3 (2016)
JCR rank
37/256 (2016)
JCR quartile
1 (2016)
ISSN
1178-2013
DOI
10.2147/IJN.S101881
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
8029127
handle
http://hdl.handle.net/1854/LU-8029127
date created
2016-07-07 11:08:09
date last changed
2017-02-10 15:35:10
@article{8029127,
  abstract     = {Designing efficient recombinant mucosal vaccines against enteric diseases is still a major challenge. Mucosal delivery of recombinant vaccines requires encapsulation in potent immunostimulatory particles to induce an efficient immune response. This paper evaluates the capacity of beta-glucan microparticles (GPs) as antigen vehicles and characterizes their immune-stimulatory effects. The relevant infectious antigen FedF was chosen to be loaded inside the microparticles. The incorporation of FedF inside the particles was highly efficient (roughly 85\%) and occurred without antigen degradation. In addition, these GPs have immunostimulatory effects as well, demonstrated by the strong reactive oxygen species (ROS) production by porcine neutrophils upon their recognition. Although antigen-loaded GPs still induce ROS production, antigen loading decreases this production by neutrophils for reasons yet unknown. However, these antigen-loaded GPs are still able to bind their specific beta-glucan receptor, demonstrated by blocking complement receptor 3, which is the major beta-glucan receptor on porcine neutrophils. The dual character of these particles is confirmed by a T-cell proliferation assay. FedF-loaded particles induce a significantly higher FedF-specific T-cell proliferation than soluble FedF. Taken together, these results show that GPs are efficient antigen carriers with immune-stimulatory properties.},
  author       = {Baert, Kim and De Geest, Bruno and De Greve, Henri and Cox, Eric and Devriendt, Bert},
  issn         = {1178-2013},
  journal      = {INTERNATIONAL JOURNAL OF NANOMEDICINE},
  keyword      = {antigen delivery vehicle,\ensuremath{\beta}-glucan microparticles,FedF,immunostimulants,FIMBRIAL ADHESIN FEDF,DELIVERY,VACCINE,BINDING,CELLS,COLI,IMMUNIZATION,COMPLEMENT,PIGLETS,SYSTEM},
  language     = {eng},
  pages        = {2463--2469},
  title        = {Duality of \ensuremath{\beta}-glucan microparticles: antigen carrier and immunostimulants},
  url          = {http://dx.doi.org/10.2147/IJN.S101881},
  volume       = {11},
  year         = {2016},
}

Chicago
Baert, Kim, Bruno De Geest, Henri De Greve, Eric Cox, and Bert Devriendt. 2016. “Duality of Β-glucan Microparticles: Antigen Carrier and Immunostimulants.” International Journal of Nanomedicine 11: 2463–2469.
APA
Baert, Kim, De Geest, B., De Greve, H., Cox, E., & Devriendt, B. (2016). Duality of β-glucan microparticles: antigen carrier and immunostimulants. INTERNATIONAL JOURNAL OF NANOMEDICINE, 11, 2463–2469.
Vancouver
1.
Baert K, De Geest B, De Greve H, Cox E, Devriendt B. Duality of β-glucan microparticles: antigen carrier and immunostimulants. INTERNATIONAL JOURNAL OF NANOMEDICINE. 2016;11:2463–9.
MLA
Baert, Kim, Bruno De Geest, Henri De Greve, et al. “Duality of Β-glucan Microparticles: Antigen Carrier and Immunostimulants.” INTERNATIONAL JOURNAL OF NANOMEDICINE 11 (2016): 2463–2469. Print.