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The C-terminal domain of Clostridium perfringens alpha toxin as a vaccine candidate against bovine necrohemorrhagic enteritis

Evy Goossens UGent, Stefanie Verherstraeten, Bram Valgaeren, Bart Pardon UGent, Leen Timbermont, Stijn Schauvliege UGent, Diego Rodrigo, Freddy Haesebrouck UGent, Richard Ducatelle UGent, Piet Deprez UGent, et al. (2016) VETERINARY RESEARCH. 47.
abstract
Bovine necrohemorrhagic enteritis is caused by Clostridium perfringens and leads to sudden death. Alpha toxin, together with perfringolysin O, has been identified as the principal toxin involved in the pathogenesis. We assessed the potential of alpha toxin as a vaccine antigen. Using an intestinal loop model in calves, we investigated the protection afforded by antisera raised against native alpha toxin or its non-toxic C-terminal fragment against C. perfringens-induced intestinal necrosis. Immunization of calves with either of the vaccine preparations induced a strong antibody response. The resulting antisera were able to neutralize the alpha toxin activity and the C. perfringens-induced endothelial cytotoxicity in vitro. The antisera raised against the native toxin had a stronger neutralizing activity than those against the C-terminal fragment. However, antibodies against alpha toxin alone were not sufficient to completely neutralize the C. perfringens-induced necrosis in the intestinal loop model. The development of a multivalent vaccine combining the C-terminal fragment of alpha toxin with other C. perfringens virulence factors might be necessary for complete protection against bovine necrohemorrhagic enteritis.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
MEDIATED GAS-GANGRENE, ENDOTHELIAL-CELLS, AVIAN NECROTIC ENTERITIS, BROILER-CHICKENS, LOOP MODEL, CALVES, ENTEROTOXEMIA, IMMUNIZATION, VIRULENCE, PERFRINGOLYSIN O
journal title
VETERINARY RESEARCH
Vet. Res.
volume
47
article number
52
pages
9 pages
Web of Science type
Article
Web of Science id
000374827300001
JCR category
VETERINARY SCIENCES
JCR impact factor
2.798 (2016)
JCR rank
5/136 (2016)
JCR quartile
1 (2016)
ISSN
0928-4249
DOI
10.1186/s13567-016-0336-y
language
English
UGent publication?
yes
classification
A1
copyright statement
Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
id
8027351
handle
http://hdl.handle.net/1854/LU-8027351
date created
2016-07-05 15:52:29
date last changed
2017-06-07 14:21:41
@article{8027351,
  abstract     = {Bovine necrohemorrhagic enteritis is caused by Clostridium perfringens and leads to sudden death. Alpha toxin, together with perfringolysin O, has been identified as the principal toxin involved in the pathogenesis. We assessed the potential of alpha toxin as a vaccine antigen. Using an intestinal loop model in calves, we investigated the protection afforded by antisera raised against native alpha toxin or its non-toxic C-terminal fragment against C. perfringens-induced intestinal necrosis. Immunization of calves with either of the vaccine preparations induced a strong antibody response. The resulting antisera were able to neutralize the alpha toxin activity and the C. perfringens-induced endothelial cytotoxicity in vitro. The antisera raised against the native toxin had a stronger neutralizing activity than those against the C-terminal fragment. However, antibodies against alpha toxin alone were not sufficient to completely neutralize the C. perfringens-induced necrosis in the intestinal loop model. The development of a multivalent vaccine combining the C-terminal fragment of alpha toxin with other C. perfringens virulence factors might be necessary for complete protection against bovine necrohemorrhagic enteritis.},
  articleno    = {52},
  author       = {Goossens, Evy and Verherstraeten, Stefanie and Valgaeren, Bram and Pardon, Bart and Timbermont, Leen and Schauvliege, Stijn and Rodrigo, Diego and Haesebrouck, Freddy and Ducatelle, Richard and Deprez, Piet and Van Immerseel, Filip},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keyword      = {MEDIATED GAS-GANGRENE,ENDOTHELIAL-CELLS,AVIAN NECROTIC ENTERITIS,BROILER-CHICKENS,LOOP MODEL,CALVES,ENTEROTOXEMIA,IMMUNIZATION,VIRULENCE,PERFRINGOLYSIN O},
  language     = {eng},
  pages        = {9},
  title        = {The C-terminal domain of Clostridium perfringens alpha toxin as a vaccine candidate against bovine necrohemorrhagic enteritis},
  url          = {http://dx.doi.org/10.1186/s13567-016-0336-y},
  volume       = {47},
  year         = {2016},
}

Chicago
Goossens, Evy, Stefanie Verherstraeten, Bram Valgaeren, Bart Pardon, Leen Timbermont, Stijn Schauvliege, Diego Rodrigo, et al. 2016. “The C-terminal Domain of Clostridium Perfringens Alpha Toxin as a Vaccine Candidate Against Bovine Necrohemorrhagic Enteritis.” Veterinary Research 47.
APA
Goossens, Evy, Verherstraeten, S., Valgaeren, B., Pardon, B., Timbermont, L., Schauvliege, S., Rodrigo, D., et al. (2016). The C-terminal domain of Clostridium perfringens alpha toxin as a vaccine candidate against bovine necrohemorrhagic enteritis. VETERINARY RESEARCH, 47.
Vancouver
1.
Goossens E, Verherstraeten S, Valgaeren B, Pardon B, Timbermont L, Schauvliege S, et al. The C-terminal domain of Clostridium perfringens alpha toxin as a vaccine candidate against bovine necrohemorrhagic enteritis. VETERINARY RESEARCH. 2016;47.
MLA
Goossens, Evy, Stefanie Verherstraeten, Bram Valgaeren, et al. “The C-terminal Domain of Clostridium Perfringens Alpha Toxin as a Vaccine Candidate Against Bovine Necrohemorrhagic Enteritis.” VETERINARY RESEARCH 47 (2016): n. pag. Print.