Advanced search
1 file | 1.52 MB

The C-terminal domain of Clostridium perfringens alpha toxin as a vaccine candidate against bovine necrohemorrhagic enteritis

Evy Goossens (UGent) , Stefanie Verherstraeten (UGent) , Bonnie Valgaeren (UGent) , Bart Pardon (UGent) , Leen Timbermont (UGent) , Stijn Schauvliege (UGent) , Diego Rodrigo, Freddy Haesebrouck (UGent) , Richard Ducatelle (UGent) , Piet Deprez (UGent) , et al.
Author
Organization
Abstract
Bovine necrohemorrhagic enteritis is caused by Clostridium perfringens and leads to sudden death. Alpha toxin, together with perfringolysin O, has been identified as the principal toxin involved in the pathogenesis. We assessed the potential of alpha toxin as a vaccine antigen. Using an intestinal loop model in calves, we investigated the protection afforded by antisera raised against native alpha toxin or its non-toxic C-terminal fragment against C. perfringens-induced intestinal necrosis. Immunization of calves with either of the vaccine preparations induced a strong antibody response. The resulting antisera were able to neutralize the alpha toxin activity and the C. perfringens-induced endothelial cytotoxicity in vitro. The antisera raised against the native toxin had a stronger neutralizing activity than those against the C-terminal fragment. However, antibodies against alpha toxin alone were not sufficient to completely neutralize the C. perfringens-induced necrosis in the intestinal loop model. The development of a multivalent vaccine combining the C-terminal fragment of alpha toxin with other C. perfringens virulence factors might be necessary for complete protection against bovine necrohemorrhagic enteritis.
Keywords
MEDIATED GAS-GANGRENE, ENDOTHELIAL-CELLS, AVIAN NECROTIC ENTERITIS, BROILER-CHICKENS, LOOP MODEL, CALVES, ENTEROTOXEMIA, IMMUNIZATION, VIRULENCE, PERFRINGOLYSIN O

Downloads

  • c-termin.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 1.52 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Goossens, Evy, Stefanie Verherstraeten, Bonnie Valgaeren, Bart Pardon, Leen Timbermont, Stijn Schauvliege, Diego Rodrigo, et al. 2016. “The C-terminal Domain of Clostridium Perfringens Alpha Toxin as a Vaccine Candidate Against Bovine Necrohemorrhagic Enteritis.” Veterinary Research 47.
APA
Goossens, Evy, Verherstraeten, S., Valgaeren, B., Pardon, B., Timbermont, L., Schauvliege, S., Rodrigo, D., et al. (2016). The C-terminal domain of Clostridium perfringens alpha toxin as a vaccine candidate against bovine necrohemorrhagic enteritis. VETERINARY RESEARCH, 47.
Vancouver
1.
Goossens E, Verherstraeten S, Valgaeren B, Pardon B, Timbermont L, Schauvliege S, et al. The C-terminal domain of Clostridium perfringens alpha toxin as a vaccine candidate against bovine necrohemorrhagic enteritis. VETERINARY RESEARCH. 2016;47.
MLA
Goossens, Evy, Stefanie Verherstraeten, Bonnie Valgaeren, et al. “The C-terminal Domain of Clostridium Perfringens Alpha Toxin as a Vaccine Candidate Against Bovine Necrohemorrhagic Enteritis.” VETERINARY RESEARCH 47 (2016): n. pag. Print.
@article{8027351,
  abstract     = {Bovine necrohemorrhagic enteritis is caused by Clostridium perfringens and leads to sudden death. Alpha toxin, together with perfringolysin O, has been identified as the principal toxin involved in the pathogenesis. We assessed the potential of alpha toxin as a vaccine antigen. Using an intestinal loop model in calves, we investigated the protection afforded by antisera raised against native alpha toxin or its non-toxic C-terminal fragment against C. perfringens-induced intestinal necrosis. Immunization of calves with either of the vaccine preparations induced a strong antibody response. The resulting antisera were able to neutralize the alpha toxin activity and the C. perfringens-induced endothelial cytotoxicity in vitro. The antisera raised against the native toxin had a stronger neutralizing activity than those against the C-terminal fragment. However, antibodies against alpha toxin alone were not sufficient to completely neutralize the C. perfringens-induced necrosis in the intestinal loop model. The development of a multivalent vaccine combining the C-terminal fragment of alpha toxin with other C. perfringens virulence factors might be necessary for complete protection against bovine necrohemorrhagic enteritis.},
  articleno    = {52},
  author       = {Goossens, Evy and Verherstraeten, Stefanie and Valgaeren, Bonnie and Pardon, Bart and Timbermont, Leen and Schauvliege, Stijn and Rodrigo, Diego and Haesebrouck, Freddy and Ducatelle, Richard and Deprez, Piet and Van Immerseel, Filip},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keyword      = {MEDIATED GAS-GANGRENE,ENDOTHELIAL-CELLS,AVIAN NECROTIC ENTERITIS,BROILER-CHICKENS,LOOP MODEL,CALVES,ENTEROTOXEMIA,IMMUNIZATION,VIRULENCE,PERFRINGOLYSIN O},
  language     = {eng},
  pages        = {9},
  title        = {The C-terminal domain of Clostridium perfringens alpha toxin as a vaccine candidate against bovine necrohemorrhagic enteritis},
  url          = {http://dx.doi.org/10.1186/s13567-016-0336-y},
  volume       = {47},
  year         = {2016},
}

Altmetric
View in Altmetric
Web of Science
Times cited: