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Alternatively activated macrophages engage in homotypic and heterotypic interactions through IL-4 and polyamine-induced E-cadherin/catenin complexes

(2009) BLOOD. 114(21). p.4664-4674
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Abstract
Alternatively activated macrophages (AAMs), triggered by interleukin-4 (IL-4) and IL-13, play a modulating role during Th2 cytokine-driven pathologies, but their molecular armament remains poorly characterized. Here, we established E-cadherin (Cdh1) as a selective marker for IL-4/IL-13 exposed mouse and human macrophages, which is STAT6-dependently induced during polarized Th2 responses associated with Taenia crassiceps helminth infections or allergic airway inflammation. The IL-4-dependent, arginase-1/ornithine decarboxylase-mediated production of polyamines is important for maximal Cdh1 induction, unveiling a novel mechanism for IL-4-dependent gene transcription. At the macrophage surface, E-cadherin forms a functional complex with the catenins that accumulates at sites of cell contact. Macrophage-specific deletion of the Cdh1 gene illustrates the implication of E-cadherin in IL-4-driven macrophage fusion and heterotypic interactions with CD103(+) and KLRG1(+) T cells. This study identifies the E-cadherin/catenin complex as a discriminative, partly polyamine-regulated feature of IL-4/IL-13-exposed alternatively activated macrophages that contributes to homotypic and heterotypic cellular interactions.
Keywords
CELL-ADHESION, GENE-EXPRESSION, DENDRITIC CELLS, CUTTING EDGE, T-CELLS, IN-VIVO, INTERLEUKIN-4, MECHANISM, CD103, INTEGRIN

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Chicago
Van den Bossche, J, Pieter Bogaert, Jolanda van Hengel, Chris Guerin, Geert Berx, K Movahedi, R Van den Bergh, et al. 2009. “Alternatively Activated Macrophages Engage in Homotypic and Heterotypic Interactions Through IL-4 and Polyamine-induced E-cadherin/catenin Complexes.” Blood 114 (21): 4664–4674.
APA
Van den Bossche, J, Bogaert, P., van Hengel, J., Guerin, C., Berx, G., Movahedi, K., Van den Bergh, R., et al. (2009). Alternatively activated macrophages engage in homotypic and heterotypic interactions through IL-4 and polyamine-induced E-cadherin/catenin complexes. BLOOD, 114(21), 4664–4674.
Vancouver
1.
Van den Bossche J, Bogaert P, van Hengel J, Guerin C, Berx G, Movahedi K, et al. Alternatively activated macrophages engage in homotypic and heterotypic interactions through IL-4 and polyamine-induced E-cadherin/catenin complexes. BLOOD. WASHINGTON: AMER SOC HEMATOLOGY; 2009;114(21):4664–74.
MLA
Van den Bossche, J et al. “Alternatively Activated Macrophages Engage in Homotypic and Heterotypic Interactions Through IL-4 and Polyamine-induced E-cadherin/catenin Complexes.” BLOOD 114.21 (2009): 4664–4674. Print.
@article{799524,
  abstract     = {Alternatively activated macrophages (AAMs), triggered by interleukin-4 (IL-4) and IL-13, play a modulating role during Th2 cytokine-driven pathologies, but their molecular armament remains poorly characterized. Here, we established E-cadherin (Cdh1) as a selective marker for IL-4/IL-13 exposed mouse and human macrophages, which is STAT6-dependently induced during polarized Th2 responses associated with Taenia crassiceps helminth infections or allergic airway inflammation. The IL-4-dependent, arginase-1/ornithine decarboxylase-mediated production of polyamines is important for maximal Cdh1 induction, unveiling a novel mechanism for IL-4-dependent gene transcription. At the macrophage surface, E-cadherin forms a functional complex with the catenins that accumulates at sites of cell contact. Macrophage-specific deletion of the Cdh1 gene illustrates the implication of E-cadherin in IL-4-driven macrophage fusion and heterotypic interactions with CD103(+) and KLRG1(+) T cells. This study identifies the E-cadherin/catenin complex as a discriminative, partly polyamine-regulated feature of IL-4/IL-13-exposed alternatively activated macrophages that contributes to homotypic and heterotypic cellular interactions.},
  author       = {Van den Bossche, J and Bogaert, Pieter and van Hengel, Jolanda and Guerin, Chris and Berx, Geert and Movahedi, K and Van den Bergh, R and Pereira-Fernandes, A and Geuns, JMC and Pircher, H and Dorny, P and Grooten, Johan and De Baetselier, P and Van Ginderachter, JA},
  issn         = {0006-4971},
  journal      = {BLOOD},
  keywords     = {CELL-ADHESION,GENE-EXPRESSION,DENDRITIC CELLS,CUTTING EDGE,T-CELLS,IN-VIVO,INTERLEUKIN-4,MECHANISM,CD103,INTEGRIN},
  language     = {eng},
  number       = {21},
  pages        = {4664--4674},
  publisher    = {AMER SOC HEMATOLOGY},
  title        = {Alternatively activated macrophages engage in homotypic and heterotypic interactions through IL-4 and polyamine-induced E-cadherin/catenin complexes},
  url          = {http://dx.doi.org/10.1182/blood-2009-05-221598},
  volume       = {114},
  year         = {2009},
}

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