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Isolation of Enterobacter cowanii from Eucalyptus showing symptoms of bacterial blight and dieback in Uruguay

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Abstract
Aims: This study was performed to identify bacterial strains isolated simultaneously with Pantoea species from Eucalyptus trees showing symptoms of bacterial blight and dieback in Uruguay. Methods and Results: Several molecular techniques including 16S rRNA and rpoB gene sequencing and DNA-DNA hybridization were used to characterize the Gram-negative, facultatively anaerobic, slime-producing bacterial strains isolated along with Pantoea species from Eucalyptus. Hypersensitivity reactions (HR) and pathogenicity tests were performed on tobacco and Eucalyptus seedlings, respectively. The isolates clustered closely with the type strain of Enterobacter cowanii in both phylogenetic trees constructed. The DNA-DNA similarity between the isolates and the type strain of Ent. cowanii ranged from 88% to 92%. A positive HR was observed on the tobacco seedlings, but no disease symptoms were visible on the inoculated Eucalyptus seedlings. Conclusions: Enterobacter cowanii was isolated from trees with symptoms of bacterial blight although strains of this bacterial species do not appear to be the causal agent of the disease. Significance and Impact of the Study: This study provides the first report of Ent. cowanii isolated from Eucalyptus. Its presence in Eucalyptus tissue suggests that it is an endophyte in trees showing symptoms of blight.
Keywords
Eucalyptus, Enterobacteriaceae, DEOXYRIBONUCLEIC-ACID, ENVIRONMENT, DNA, IDENTIFICATION, HYBRIDIZATION, CLOACAE, PANTOEA, SP NOV., bacterial blight, endophyte, Enterobacter cowanii

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MLA
Brady, CL et al. “Isolation of Enterobacter Cowanii from Eucalyptus Showing Symptoms of Bacterial Blight and Dieback in Uruguay.” LETTERS IN APPLIED MICROBIOLOGY 49.4 (2009): 461–465. Print.
APA
Brady, CL, Venter, S., Cleenwerck, I., Engelbeen, K., De Vos, P., Wingfield, M., Telechea, L., et al. (2009). Isolation of Enterobacter cowanii from Eucalyptus showing symptoms of bacterial blight and dieback in Uruguay. LETTERS IN APPLIED MICROBIOLOGY, 49(4), 461–465.
Chicago author-date
Brady, CL, SN Venter, Ilse Cleenwerck, Katrien Engelbeen, Paul De Vos, MJ Wingfield, L Telechea, and TA Couthino. 2009. “Isolation of Enterobacter Cowanii from Eucalyptus Showing Symptoms of Bacterial Blight and Dieback in Uruguay.” Letters in Applied Microbiology 49 (4): 461–465.
Chicago author-date (all authors)
Brady, CL, SN Venter, Ilse Cleenwerck, Katrien Engelbeen, Paul De Vos, MJ Wingfield, L Telechea, and TA Couthino. 2009. “Isolation of Enterobacter Cowanii from Eucalyptus Showing Symptoms of Bacterial Blight and Dieback in Uruguay.” Letters in Applied Microbiology 49 (4): 461–465.
Vancouver
1.
Brady C, Venter S, Cleenwerck I, Engelbeen K, De Vos P, Wingfield M, et al. Isolation of Enterobacter cowanii from Eucalyptus showing symptoms of bacterial blight and dieback in Uruguay. LETTERS IN APPLIED MICROBIOLOGY. 2009;49(4):461–5.
IEEE
[1]
C. Brady et al., “Isolation of Enterobacter cowanii from Eucalyptus showing symptoms of bacterial blight and dieback in Uruguay,” LETTERS IN APPLIED MICROBIOLOGY, vol. 49, no. 4, pp. 461–465, 2009.
@article{799113,
  abstract     = {{Aims: This study was performed to identify bacterial strains isolated simultaneously with Pantoea species from Eucalyptus trees showing symptoms of bacterial blight and dieback in Uruguay.
Methods and Results: Several molecular techniques including 16S rRNA and rpoB gene sequencing and DNA-DNA hybridization were used to characterize the Gram-negative, facultatively anaerobic, slime-producing bacterial strains isolated along with Pantoea species from Eucalyptus. Hypersensitivity reactions (HR) and pathogenicity tests were performed on tobacco and Eucalyptus seedlings, respectively. The isolates clustered closely with the type strain of Enterobacter cowanii in both phylogenetic trees constructed. The DNA-DNA similarity between the isolates and the type strain of Ent. cowanii ranged from 88% to 92%. A positive HR was observed on the tobacco seedlings, but no disease symptoms were visible on the inoculated Eucalyptus seedlings.
Conclusions: Enterobacter cowanii was isolated from trees with symptoms of bacterial blight although strains of this bacterial species do not appear to be the causal agent of the disease.
Significance and Impact of the Study: This study provides the first report of Ent. cowanii isolated from Eucalyptus. Its presence in Eucalyptus tissue suggests that it is an endophyte in trees showing symptoms of blight.}},
  author       = {{Brady, CL and Venter, SN and Cleenwerck, Ilse and Engelbeen, Katrien and De Vos, Paul and Wingfield, MJ and Telechea, L and Couthino, TA}},
  issn         = {{0266-8254}},
  journal      = {{LETTERS IN APPLIED MICROBIOLOGY}},
  keywords     = {{Eucalyptus,Enterobacteriaceae,DEOXYRIBONUCLEIC-ACID,ENVIRONMENT,DNA,IDENTIFICATION,HYBRIDIZATION,CLOACAE,PANTOEA,SP NOV.,bacterial blight,endophyte,Enterobacter cowanii}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{461--465}},
  title        = {{Isolation of Enterobacter cowanii from Eucalyptus showing symptoms of bacterial blight and dieback in Uruguay}},
  url          = {{http://dx.doi.org/10.1111/j.1472-765X.2009.02692.x}},
  volume       = {{49}},
  year         = {{2009}},
}

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