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Differentiation of species of the family Acetobacteraceae by AFLP DNA fingerprinting: Gluconacetobacter kombuchae is a later heterotypic synonym of Gluconacetobacter hansenii

Ilse Cleenwerck UGent, Marjan De Wachter UGent, Angel González, Luc De Vuyst and Paul De Vos UGent (2009) INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 59(7). p.1771-1786
abstract
Amplified fragment length polymorphism (AFLP) DNA fingerprinting was investigated as a tool for fast and accurate identification of acetic acid bacteria (AAB) to the species level. One hundred and thirty five reference strains and 15 additional strains, representing 50 recognized species of the family Acetobacteraceae, were subjected to AFLP analysis using the restriction enzyme combination Apal/Taql and the primer combination A03/T03. The reference strains had been previously subjected to either DNA-DNA hybridization or 16S-23S rRNA spacer region gene sequence analysis and were regarded as being accurately classified at the species level. The present study revealed that six of these strains should be reclassified, namely Gluconacetobacter europaeus LMG 1518 and Gluconacetobacter xylinus LMG 1510 as Gluconacetobacter xylinus and Gluconacetobacter europaeus, respectively; Gluconacetobacter kombuchae LMG 237267 as Gluconacetobacter hansenii; and Acetobacter orleanensis strains LMG 1545, LMG 1592 and LMG 1608 as Acetobacter cerevisiae. Cluster analysis of the AFLP DNA fingerprints of the reference strains revealed one cluster for each species, showing a linkage level below 50% with other clusters, except for Acetobacter pasteurianus, Acetobacter indonesiensis and Acetobacter cerevisiae. These three species were separated into two, two, and three clusters, respectively. At present, confusion exists regarding the taxonomic status of Gluconacetobacter oboediens and Gluconacetobacter intermedius; the AFLP data from this study supported their classification as separate taxa. The 15 additional strains could all be identified at the species level. AFLP analysis further revealed that some species harboured genetically diverse strains, whereas other species consisted of strains showing similar banding patterns, indicating a more limited genetic diversity. It can be concluded that AFLP DNA fingerprinting is suitable for accurate identification and classification of a broad range of AAB, as well as for the determination of intraspecific genetic diversity.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
DEOXYRIBONUCLEIC-ACID, ASAIA-BOGORENSIS, COCOA BEANS, GEN. NOV., SP-NOV., ALPHA-PROTEOBACTERIA, FRAGMENT LENGTH POLYMORPHISM, AD-HOC-COMMITTEE, ACETIC-ACID BACTERIUM, INDUSTRIAL VINEGAR FERMENTATIONS
journal title
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Int. J. Syst. Evol. Microbiol.
volume
59
issue
7
pages
1771 - 1786
Web of Science type
Article
Web of Science id
000268219800038
JCR category
MICROBIOLOGY
JCR impact factor
2.113 (2009)
JCR rank
56/94 (2009)
JCR quartile
3 (2009)
ISSN
1466-5026
DOI
10.1099/ijs.0.005157-0
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
799084
handle
http://hdl.handle.net/1854/LU-799084
date created
2009-12-04 09:51:22
date last changed
2016-12-19 15:46:56
@article{799084,
  abstract     = {Amplified fragment length polymorphism (AFLP) DNA fingerprinting was investigated as a tool for fast and accurate identification of acetic acid bacteria (AAB) to the species level. One hundred and thirty five reference strains and 15 additional strains, representing 50 recognized species of the family Acetobacteraceae, were subjected to AFLP analysis using the restriction enzyme combination Apal/Taql and the primer combination A03/T03. The reference strains had been previously subjected to either DNA-DNA hybridization or 16S-23S rRNA spacer region gene sequence analysis and were regarded as being accurately classified at the species level. The present study revealed that six of these strains should be reclassified, namely Gluconacetobacter europaeus LMG 1518 and Gluconacetobacter xylinus LMG 1510 as Gluconacetobacter xylinus and Gluconacetobacter europaeus, respectively; Gluconacetobacter kombuchae LMG 237267 as Gluconacetobacter hansenii; and Acetobacter orleanensis strains LMG 1545, LMG 1592 and LMG 1608 as Acetobacter cerevisiae. Cluster analysis of the AFLP DNA fingerprints of the reference strains revealed one cluster for each species, showing a linkage level below 50\% with other clusters, except for Acetobacter pasteurianus, Acetobacter indonesiensis and Acetobacter cerevisiae. These three species were separated into two, two, and three clusters, respectively. At present, confusion exists regarding the taxonomic status of Gluconacetobacter oboediens and Gluconacetobacter intermedius; the AFLP data from this study supported their classification as separate taxa. The 15 additional strains could all be identified at the species level. AFLP analysis further revealed that some species harboured genetically diverse strains, whereas other species consisted of strains showing similar banding patterns, indicating a more limited genetic diversity. It can be concluded that AFLP DNA fingerprinting is suitable for accurate identification and classification of a broad range of AAB, as well as for the determination of intraspecific genetic diversity.},
  author       = {Cleenwerck, Ilse and De Wachter, Marjan and Gonz{\'a}lez, Angel and De Vuyst, Luc and De Vos, Paul},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keyword      = {DEOXYRIBONUCLEIC-ACID,ASAIA-BOGORENSIS,COCOA BEANS,GEN. NOV.,SP-NOV.,ALPHA-PROTEOBACTERIA,FRAGMENT LENGTH POLYMORPHISM,AD-HOC-COMMITTEE,ACETIC-ACID BACTERIUM,INDUSTRIAL VINEGAR FERMENTATIONS},
  language     = {eng},
  number       = {7},
  pages        = {1771--1786},
  title        = {Differentiation of species of the family Acetobacteraceae by AFLP DNA fingerprinting: Gluconacetobacter kombuchae is a later heterotypic synonym of Gluconacetobacter hansenii},
  url          = {http://dx.doi.org/10.1099/ijs.0.005157-0},
  volume       = {59},
  year         = {2009},
}

Chicago
Cleenwerck, Ilse, Marjan De Wachter, Angel González, Luc De Vuyst, and Paul De Vos. 2009. “Differentiation of Species of the Family Acetobacteraceae by AFLP DNA Fingerprinting: Gluconacetobacter Kombuchae Is a Later Heterotypic Synonym of Gluconacetobacter Hansenii.” International Journal of Systematic and Evolutionary Microbiology 59 (7): 1771–1786.
APA
Cleenwerck, I., De Wachter, M., González, A., De Vuyst, L., & De Vos, P. (2009). Differentiation of species of the family Acetobacteraceae by AFLP DNA fingerprinting: Gluconacetobacter kombuchae is a later heterotypic synonym of Gluconacetobacter hansenii. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 59(7), 1771–1786.
Vancouver
1.
Cleenwerck I, De Wachter M, González A, De Vuyst L, De Vos P. Differentiation of species of the family Acetobacteraceae by AFLP DNA fingerprinting: Gluconacetobacter kombuchae is a later heterotypic synonym of Gluconacetobacter hansenii. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2009;59(7):1771–86.
MLA
Cleenwerck, Ilse, Marjan De Wachter, Angel González, et al. “Differentiation of Species of the Family Acetobacteraceae by AFLP DNA Fingerprinting: Gluconacetobacter Kombuchae Is a Later Heterotypic Synonym of Gluconacetobacter Hansenii.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 59.7 (2009): 1771–1786. Print.