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(Homo)glutathione Depletion Modulates Host Gene Expression during the Symbiotic Interaction between Medicago truncatula and Sinorhizobium meliloti

Chiara Pucciariello, Gilles Innocenti, Willem Van De Velde UGent, Annie Lambert, Julie Hopkins, Mathilde Clément, Michel Ponchet, Nicolas Pauly, Sofie Goormachtig UGent, Marcella Holsters UGent, et al. (2009) PLANT PHYSIOLOGY. 151(3). p.1186-1196
abstract
Under nitrogen-limiting conditions, legumes interact with symbiotic rhizobia to produce nitrogen-fixing root nodules. We have previously shown that glutathione and homoglutathione [(h)GSH] deficiencies impaired Medicago truncatula symbiosis efficiency, showing the importance of the low M-r thiols during the nodulation process in the model legume M. truncatula. In this study, the plant transcriptomic response to Sinorhizobium meliloti infection under (h)GSH depletion was investigated using cDNA-amplified fragment length polymorphism analysis. Among 6,149 expression tags monitored, 181 genes displayed significant differential expression between inoculated control and inoculated (h)GSH depleted roots. Quantitative reverse transcription polymerase chain reaction analysis confirmed the changes in mRNA levels. This transcriptomic analysis shows a down-regulation of genes involved in meristem formation and a modulation of the expression of stress-related genes in (h)GSH-depleted plants. Promoter-beta-glucuronidase histochemical analysis showed that the putative MtPIP2 aquaporin might be up-regulated during nodule meristem formation and that this up-regulation is inhibited under (h)GSH depletion. (h)GSH depletion enhances the expression of salicylic acid (SA)-regulated genes after S. meliloti infection and the expression of SA-regulated genes after exogenous SA treatment. Modification of water transport and SA signaling pathway observed under (h)GSH deficiency contribute to explain how (h)GSH depletion alters the proper development of the symbiotic interaction.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
keyword
SIGNALING NETWORK, PLANT DEFENSE, SALICYLIC-ACID, LEGUME-RHIZOBIA SYMBIOSIS, SYSTEMIC ACQUIRED-RESISTANCE, ROOT, AUXIN TRANSPORT REGULATION, CELL-DIVISION, NODULATION, REDOX CONTROL
journal title
PLANT PHYSIOLOGY
Plant Physiol.
volume
151
issue
3
pages
11 pages
publisher
AMER SOC PLANT BIOLOGISTS
place of publication
ROCKVILLE
Web of Science type
Article
Web of Science id
000271430500023
JCR category
PLANT SCIENCES
JCR impact factor
6.235 (2009)
JCR rank
7/172 (2009)
JCR quartile
1 (2009)
ISSN
0032-0889
DOI
10.1104/pp.109.142034
language
English
UGent publication?
yes
classification
A1
copyright statement
I don't know the status of the copyright for this publication
id
798247
handle
http://hdl.handle.net/1854/LU-798247
date created
2009-12-02 17:35:24
date last changed
2017-01-02 09:55:55
@article{798247,
  abstract     = {Under nitrogen-limiting conditions, legumes interact with symbiotic rhizobia to produce nitrogen-fixing root nodules. We have previously shown that glutathione and homoglutathione [(h)GSH] deficiencies impaired Medicago truncatula symbiosis efficiency, showing the importance of the low M-r thiols during the nodulation process in the model legume M. truncatula. In this study, the plant transcriptomic response to Sinorhizobium meliloti infection under (h)GSH depletion was investigated using cDNA-amplified fragment length polymorphism analysis. Among 6,149 expression tags monitored, 181 genes displayed significant differential expression between inoculated control and inoculated (h)GSH depleted roots. Quantitative reverse transcription polymerase chain reaction analysis confirmed the changes in mRNA levels. This transcriptomic analysis shows a down-regulation of genes involved in meristem formation and a modulation of the expression of stress-related genes in (h)GSH-depleted plants. Promoter-beta-glucuronidase histochemical analysis showed that the putative MtPIP2 aquaporin might be up-regulated during nodule meristem formation and that this up-regulation is inhibited under (h)GSH depletion. (h)GSH depletion enhances the expression of salicylic acid (SA)-regulated genes after S. meliloti infection and the expression of SA-regulated genes after exogenous SA treatment. Modification of water transport and SA signaling pathway observed under (h)GSH deficiency contribute to explain how (h)GSH depletion alters the proper development of the symbiotic interaction.},
  author       = {Pucciariello, Chiara and Innocenti, Gilles and Van De Velde, Willem and Lambert, Annie and Hopkins, Julie and Cl{\'e}ment, Mathilde and Ponchet, Michel and Pauly, Nicolas and Goormachtig, Sofie and Holsters, Marcella and Puppo, Alain and Frendo, Pierre},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {SIGNALING NETWORK,PLANT DEFENSE,SALICYLIC-ACID,LEGUME-RHIZOBIA SYMBIOSIS,SYSTEMIC ACQUIRED-RESISTANCE,ROOT,AUXIN TRANSPORT REGULATION,CELL-DIVISION,NODULATION,REDOX CONTROL},
  language     = {eng},
  number       = {3},
  pages        = {1186--1196},
  publisher    = {AMER SOC PLANT BIOLOGISTS},
  title        = {(Homo)glutathione Depletion Modulates Host Gene Expression during the Symbiotic Interaction between Medicago truncatula and Sinorhizobium meliloti},
  url          = {http://dx.doi.org/10.1104/pp.109.142034},
  volume       = {151},
  year         = {2009},
}

Chicago
Pucciariello, Chiara, Gilles Innocenti, Willem Van De Velde, Annie Lambert, Julie Hopkins, Mathilde Clément, Michel Ponchet, et al. 2009. “(Homo)glutathione Depletion Modulates Host Gene Expression During the Symbiotic Interaction Between Medicago Truncatula and Sinorhizobium Meliloti.” Plant Physiology 151 (3): 1186–1196.
APA
Pucciariello, C., Innocenti, G., Van De Velde, W., Lambert, A., Hopkins, J., Clément, M., Ponchet, M., et al. (2009). (Homo)glutathione Depletion Modulates Host Gene Expression during the Symbiotic Interaction between Medicago truncatula and Sinorhizobium meliloti. PLANT PHYSIOLOGY, 151(3), 1186–1196.
Vancouver
1.
Pucciariello C, Innocenti G, Van De Velde W, Lambert A, Hopkins J, Clément M, et al. (Homo)glutathione Depletion Modulates Host Gene Expression during the Symbiotic Interaction between Medicago truncatula and Sinorhizobium meliloti. PLANT PHYSIOLOGY. ROCKVILLE: AMER SOC PLANT BIOLOGISTS; 2009;151(3):1186–96.
MLA
Pucciariello, Chiara, Gilles Innocenti, Willem Van De Velde, et al. “(Homo)glutathione Depletion Modulates Host Gene Expression During the Symbiotic Interaction Between Medicago Truncatula and Sinorhizobium Meliloti.” PLANT PHYSIOLOGY 151.3 (2009): 1186–1196. Print.