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Gene silencing induced by hairpin or inverted repeated sense transgenes varies among promoters and cell types

Gordana Marjanac UGent, Mansour Karimi UGent, Mirande Naudts UGent, Tom Beeckman UGent, Anna Depicker UGent and Sylvie De Buck UGent (2009) NEW PHYTOLOGIST. 184(4). p.851-864
abstract
In transgenic calli and different tissues of Arabidopsis thaliana plants, the in trans silencing capacity of a 35S-beta-glucuronidase (GUS) hairpin RNA construct was investigated on a target GUS gene, under the control of the 35S, a WRKY or several cell cycle-specific promoters. GUS histochemical staining patterns were analyzed in all tissues of the parental lines and supertransformants harboring the hairpin construct. Quantitative GUS activity measurements determined GUS suppression by a 35S-GUS hairpin or inverted repeated GUS transgenes in leaves and calli. In some supertransformants, GUS-based staining disappeared in all tissues, including calli. In most supertransformants, however, a significant reduction was found in mature roots and leaves, but residual GUS activity was observed in the root tips, young leaves and calli. In leaves of most hairpin RNA supertransformants, the GUS activity was reduced by c. 1000-fold or more, but, in derived calli, generally by less than 200-fold. The silencing efficiency of inverted repeated sense transgenes was similar to that of a hairpin RNA construct in leaves, but weaker in calli. These results imply that the tissue type, nature of the silencing inducer locus and the differential expression of the targeted gene codetermine the silencing efficiency.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
INTERFERENCE, EXPRESSION, T-DNAS, DNA METHYLATION, ARABIDOPSIS-THALIANA, PLANT-PARASITIC NEMATODES, DOUBLE-STRANDED-RNA, VECTORS, PATHWAYS, VIRUS
journal title
NEW PHYTOLOGIST
New Phytol.
volume
184
issue
4
pages
851 - 864
Web of Science type
Article
Web of Science id
000271644500012
JCR category
PLANT SCIENCES
JCR impact factor
6.033 (2009)
JCR rank
8/172 (2009)
JCR quartile
1 (2009)
ISSN
0028-646X
DOI
10.1111/j.1469-8137.2009.03011.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
798228
handle
http://hdl.handle.net/1854/LU-798228
date created
2009-12-02 17:35:11
date last changed
2013-10-21 11:12:00
@article{798228,
  abstract     = {In transgenic calli and different tissues of Arabidopsis thaliana plants, the in trans silencing capacity of a 35S-beta-glucuronidase (GUS) hairpin RNA construct was investigated on a target GUS gene, under the control of the 35S, a WRKY or several cell cycle-specific promoters.
GUS histochemical staining patterns were analyzed in all tissues of the parental lines and supertransformants harboring the hairpin construct. Quantitative GUS activity measurements determined GUS suppression by a 35S-GUS hairpin or inverted repeated GUS transgenes in leaves and calli.
In some supertransformants, GUS-based staining disappeared in all tissues, including calli. In most supertransformants, however, a significant reduction was found in mature roots and leaves, but residual GUS activity was observed in the root tips, young leaves and calli. In leaves of most hairpin RNA supertransformants, the GUS activity was reduced by c. 1000-fold or more, but, in derived calli, generally by less than 200-fold. The silencing efficiency of inverted repeated sense transgenes was similar to that of a hairpin RNA construct in leaves, but weaker in calli.
These results imply that the tissue type, nature of the silencing inducer locus and the differential expression of the targeted gene codetermine the silencing efficiency.},
  author       = {Marjanac, Gordana and Karimi, Mansour and Naudts, Mirande and Beeckman, Tom and Depicker, Anna and De Buck, Sylvie},
  issn         = {0028-646X},
  journal      = {NEW PHYTOLOGIST},
  keyword      = {INTERFERENCE,EXPRESSION,T-DNAS,DNA METHYLATION,ARABIDOPSIS-THALIANA,PLANT-PARASITIC NEMATODES,DOUBLE-STRANDED-RNA,VECTORS,PATHWAYS,VIRUS},
  language     = {eng},
  number       = {4},
  pages        = {851--864},
  title        = {Gene silencing induced by hairpin or inverted repeated sense transgenes varies among promoters and cell types},
  url          = {http://dx.doi.org/10.1111/j.1469-8137.2009.03011.x},
  volume       = {184},
  year         = {2009},
}

Chicago
Marjanac, Gordana, Mansour Karimi, Mirande Naudts, Tom Beeckman, Anna Depicker, and Sylvie De Buck. 2009. “Gene Silencing Induced by Hairpin or Inverted Repeated Sense Transgenes Varies Among Promoters and Cell Types.” New Phytologist 184 (4): 851–864.
APA
Marjanac, G., Karimi, M., Naudts, M., Beeckman, T., Depicker, A., & De Buck, S. (2009). Gene silencing induced by hairpin or inverted repeated sense transgenes varies among promoters and cell types. NEW PHYTOLOGIST, 184(4), 851–864.
Vancouver
1.
Marjanac G, Karimi M, Naudts M, Beeckman T, Depicker A, De Buck S. Gene silencing induced by hairpin or inverted repeated sense transgenes varies among promoters and cell types. NEW PHYTOLOGIST. 2009;184(4):851–64.
MLA
Marjanac, Gordana, Mansour Karimi, Mirande Naudts, et al. “Gene Silencing Induced by Hairpin or Inverted Repeated Sense Transgenes Varies Among Promoters and Cell Types.” NEW PHYTOLOGIST 184.4 (2009): 851–864. Print.