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Correlation between genotypic diversity, lipooligosaccharide gene locus class variation, and Caco-2 cell invasion potential of Campylobacter jejuni isolates from chicken meat and humans: contribution to virulotyping

Ihab Habib, Rogier Louwen, Mieke Uyttendaele UGent, Kurt Houf UGent, Olivier Vandenberg, Edward E. Nieuwenhuis, William G Miller, Alex Van Belkum and Lieven De Zutter UGent (2009) APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 75(13). p.4277-4288
abstract
Significant interest in studying the lipooligosaccharide (LOS) of Campylobacter jejuni has stemmed from its potential role in postinfection paralytic disorders. In this study we present the results of PCR screening of five LOS locus classes (A, B, C, D, and E) for a collection of 116 C. jejuni isolates from chicken meat (n = 76) and sporadic human cases of diarrhea (n = 40). We correlated LOS classes with clonal complexes (CC) assigned by multilocus sequence typing (MLST). Finally, we evaluated the invasion potential of a panel of 52 of these C. jejuni isolates for Caco-2 cells. PCR screening showed that 87.1% (101/116) of isolates could be assigned to LOS class A, B, C, D, or E. Concordance between LOS classes and certain MLST CC was revealed. The majority (85.7% [24/28]) of C. jejuni isolates grouped in CC-21 were shown to express LOS locus class C. The invasion potential of C. jejuni isolates possessing sialylated LOS (n = 29; classes A, B, and C) for Caco-2 cells was significantly higher (P < 0.0001) than that of C. jejuni isolates with nonsialylated LOS (n = 23; classes D and E). There was no significant difference in invasiveness between chicken meat and human isolates. However, C. jejuni isolates assigned to CC-206 (correlated with LOS class B) or CC-21 (correlated with LOS class C) showed statistically significantly higher levels of invasion than isolates from other CC. Correlation between LOS classes and CC was further confirmed by pulsed-field gel electrophoresis. The present study reveals a correlation between genotypic diversity and LOS locus classes of C. jejuni. We showed that simple PCR screening for C. jejuni LOS classes could reliably predict certain MLST CC and add to the interpretation of molecular-typing results. Our study corroborates that sialylation of LOS is advantageous for C. jejuni fitness and virulence in different hosts. The modulation of cell surface carbohydrate structure could enhance the ability of C. jejuni to adapt to or survive in a host.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
GUILLAIN-BARRE-SYNDROME, VITRO VIRULENCE PROPERTIES, MILLER-FISHER-SYNDROMES, SEQUENCE TYPING SYSTEM, IN-VITRO, ANTIGANGLIOSIDE ANTIBODIES, POLYMORPHISM ANALYSIS, COLI, STRAINS, IDENTIFICATION
journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Appl. Environ. Microbiol.
volume
75
issue
13
pages
4277 - 4288
Web of Science type
Article
Web of Science id
000267373000007
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
3.686 (2009)
JCR rank
28/150 (2009)
JCR quartile
1 (2009)
ISSN
0099-2240
DOI
10.1128/AEM.02269-08
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
792132
handle
http://hdl.handle.net/1854/LU-792132
date created
2009-11-30 15:10:08
date last changed
2016-12-19 15:44:44
@article{792132,
  abstract     = {Significant interest in studying the lipooligosaccharide (LOS) of Campylobacter jejuni has stemmed from its potential role in postinfection paralytic disorders. In this study we present the results of PCR screening of five LOS locus classes (A, B, C, D, and E) for a collection of 116 C. jejuni isolates from chicken meat (n = 76) and sporadic human cases of diarrhea (n = 40). We correlated LOS classes with clonal complexes (CC) assigned by multilocus sequence typing (MLST). Finally, we evaluated the invasion potential of a panel of 52 of these C. jejuni isolates for Caco-2 cells. PCR screening showed that 87.1\% (101/116) of isolates could be assigned to LOS class A, B, C, D, or E. Concordance between LOS classes and certain MLST CC was revealed. The majority (85.7\% [24/28]) of C. jejuni isolates grouped in CC-21 were shown to express LOS locus class C. The invasion potential of C. jejuni isolates possessing sialylated LOS (n = 29; classes A, B, and C) for Caco-2 cells was significantly higher (P {\textlangle} 0.0001) than that of C. jejuni isolates with nonsialylated LOS (n = 23; classes D and E). There was no significant difference in invasiveness between chicken meat and human isolates. However, C. jejuni isolates assigned to CC-206 (correlated with LOS class B) or CC-21 (correlated with LOS class C) showed statistically significantly higher levels of invasion than isolates from other CC. Correlation between LOS classes and CC was further confirmed by pulsed-field gel electrophoresis. The present study reveals a correlation between genotypic diversity and LOS locus classes of C. jejuni. We showed that simple PCR screening for C. jejuni LOS classes could reliably predict certain MLST CC and add to the interpretation of molecular-typing results. Our study corroborates that sialylation of LOS is advantageous for C. jejuni fitness and virulence in different hosts. The modulation of cell surface carbohydrate structure could enhance the ability of C. jejuni to adapt to or survive in a host.},
  author       = {Habib, Ihab and Louwen, Rogier and Uyttendaele, Mieke and Houf, Kurt and Vandenberg, Olivier and Nieuwenhuis, Edward E. and Miller, William G and Van Belkum, Alex and De Zutter, Lieven},
  issn         = {0099-2240},
  journal      = {APPLIED AND ENVIRONMENTAL MICROBIOLOGY},
  keyword      = {GUILLAIN-BARRE-SYNDROME,VITRO VIRULENCE PROPERTIES,MILLER-FISHER-SYNDROMES,SEQUENCE TYPING SYSTEM,IN-VITRO,ANTIGANGLIOSIDE ANTIBODIES,POLYMORPHISM ANALYSIS,COLI,STRAINS,IDENTIFICATION},
  language     = {eng},
  number       = {13},
  pages        = {4277--4288},
  title        = {Correlation between genotypic diversity, lipooligosaccharide gene locus class variation, and Caco-2 cell invasion potential of Campylobacter jejuni isolates from chicken meat and humans: contribution to virulotyping},
  url          = {http://dx.doi.org/10.1128/AEM.02269-08},
  volume       = {75},
  year         = {2009},
}

Chicago
Habib, Ihab, Rogier Louwen, Mieke Uyttendaele, Kurt Houf, Olivier Vandenberg, Edward E. Nieuwenhuis, William G Miller, Alex Van Belkum, and Lieven De Zutter. 2009. “Correlation Between Genotypic Diversity, Lipooligosaccharide Gene Locus Class Variation, and Caco-2 Cell Invasion Potential of Campylobacter Jejuni Isolates from Chicken Meat and Humans: Contribution to Virulotyping.” Applied and Environmental Microbiology 75 (13): 4277–4288.
APA
Habib, I., Louwen, R., Uyttendaele, M., Houf, K., Vandenberg, O., Nieuwenhuis, E. E., Miller, W. G., et al. (2009). Correlation between genotypic diversity, lipooligosaccharide gene locus class variation, and Caco-2 cell invasion potential of Campylobacter jejuni isolates from chicken meat and humans: contribution to virulotyping. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(13), 4277–4288.
Vancouver
1.
Habib I, Louwen R, Uyttendaele M, Houf K, Vandenberg O, Nieuwenhuis EE, et al. Correlation between genotypic diversity, lipooligosaccharide gene locus class variation, and Caco-2 cell invasion potential of Campylobacter jejuni isolates from chicken meat and humans: contribution to virulotyping. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 2009;75(13):4277–88.
MLA
Habib, Ihab, Rogier Louwen, Mieke Uyttendaele, et al. “Correlation Between Genotypic Diversity, Lipooligosaccharide Gene Locus Class Variation, and Caco-2 Cell Invasion Potential of Campylobacter Jejuni Isolates from Chicken Meat and Humans: Contribution to Virulotyping.” APPLIED AND ENVIRONMENTAL MICROBIOLOGY 75.13 (2009): 4277–4288. Print.