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The human homologue of Caenorhabditis elegans CED-6 specifically promotes phagocytosis of apoptotic cells

Elke Smits, Wim Van Criekinge UGent, Geert Plaetinck and Thierry Bogaert (1999) CURRENT BIOLOGY. 9(22). p.1351-1354
abstract
A key feature of the process of programmed cell death (apoptosis) is the efficiency with which the dying cells are recognized and engulfed by phagocytes [1], Apoptotic cells are rapidly cleared either by neighbouring cells acting as semi-professional phagocytes or by experts of the macrophage line, so that an inflammatory response is avoided [2]. The Caenorhabditis elegans gene ced-6 is required for efficient engulfment of apoptotic cells [3] and is one of a group of genes that define two partially redundant parallel pathways for the engulfment process [4,5]. These pathways may be conserved across evolution, as two other engulfment genes have human homologues, A CED-5 homologue is part of a human CrkII-DOCK180-Rac signaling pathway proposed to mediate cytoskeletal reorganization [6-8] and a CED-7 homologue is similar to the ABC transporters [9,10]. Here, we report the cloning and characterization of human CED-6, a human homologue of C. elegans CED-6. The 34 kDa hCED-6 protein is expressed in most tissues, some human cancer cells, and in primary human macrophages. We developed an assay that quantitates the phagocytic activity of mammalian macrophages: the number of apoptotic cells that have been internalized is measured by the uptake of lacZ-positive apoptotic cells by adherent transgenic macrophages. The results of this assay demonstrate that overexpression of hCED-6 promotes phagocytosis only of apoptotic cells and suggest that hCED-6 is the mammalian orthologue of C. elegans CED-6 and is a part of a highly conserved pathway that specifically mediates the phagocytosis of apoptotic cells.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PROTEIN, C-ELEGANS, ENGULFMENT, MACROPHAGES, RECOGNITION, NEUTROPHILS, DOCK180, BINDING, DEATH, CORPSES
journal title
CURRENT BIOLOGY
Curr. Biol.
volume
9
issue
22
pages
1351 - 1354
Web of Science type
Article
Web of Science id
000083770500028
ISSN
0960-9822
DOI
10.1016/S0960-9822(00)80062-7
language
English
UGent publication?
no
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
791265
handle
http://hdl.handle.net/1854/LU-791265
date created
2009-11-28 22:06:10
date last changed
2009-12-22 16:58:14
@article{791265,
  abstract     = {A key feature of the process of programmed cell death (apoptosis) is the efficiency with which the dying cells are recognized and engulfed by phagocytes [1], Apoptotic cells are rapidly cleared either by neighbouring cells acting as semi-professional phagocytes or by experts of the macrophage line, so that an inflammatory response is avoided [2]. The Caenorhabditis elegans gene ced-6 is required for efficient engulfment of apoptotic cells [3] and is one of a group of genes that define two partially redundant parallel pathways for the engulfment process [4,5]. These pathways may be conserved across evolution, as two other engulfment genes have human homologues, A CED-5 homologue is part of a human CrkII-DOCK180-Rac signaling pathway proposed to mediate cytoskeletal reorganization [6-8] and a CED-7 homologue is similar to the ABC transporters [9,10]. Here, we report the cloning and characterization of human CED-6, a human homologue of C. elegans CED-6. The 34 kDa hCED-6 protein is expressed in most tissues, some human cancer cells, and in primary human macrophages. We developed an assay that quantitates the phagocytic activity of mammalian macrophages: the number of apoptotic cells that have been internalized is measured by the uptake of lacZ-positive apoptotic cells by adherent transgenic macrophages. The results of this assay demonstrate that overexpression of hCED-6 promotes phagocytosis only of apoptotic cells and suggest that hCED-6 is the mammalian orthologue of C. elegans CED-6 and is a part of a highly conserved pathway that specifically mediates the phagocytosis of apoptotic cells.},
  author       = {Smits, Elke and Van Criekinge, Wim and Plaetinck, Geert and Bogaert, Thierry},
  issn         = {0960-9822},
  journal      = {CURRENT BIOLOGY},
  keyword      = {PROTEIN,C-ELEGANS,ENGULFMENT,MACROPHAGES,RECOGNITION,NEUTROPHILS,DOCK180,BINDING,DEATH,CORPSES},
  language     = {eng},
  number       = {22},
  pages        = {1351--1354},
  title        = {The human homologue of Caenorhabditis elegans CED-6 specifically promotes phagocytosis of apoptotic cells},
  url          = {http://dx.doi.org/10.1016/S0960-9822(00)80062-7},
  volume       = {9},
  year         = {1999},
}

Chicago
Smits, Elke, Wim Van Criekinge, Geert Plaetinck, and Thierry Bogaert. 1999. “The Human Homologue of Caenorhabditis Elegans CED-6 Specifically Promotes Phagocytosis of Apoptotic Cells.” Current Biology 9 (22): 1351–1354.
APA
Smits, E., Van Criekinge, W., Plaetinck, G., & Bogaert, T. (1999). The human homologue of Caenorhabditis elegans CED-6 specifically promotes phagocytosis of apoptotic cells. CURRENT BIOLOGY, 9(22), 1351–1354.
Vancouver
1.
Smits E, Van Criekinge W, Plaetinck G, Bogaert T. The human homologue of Caenorhabditis elegans CED-6 specifically promotes phagocytosis of apoptotic cells. CURRENT BIOLOGY. 1999;9(22):1351–4.
MLA
Smits, Elke, Wim Van Criekinge, Geert Plaetinck, et al. “The Human Homologue of Caenorhabditis Elegans CED-6 Specifically Promotes Phagocytosis of Apoptotic Cells.” CURRENT BIOLOGY 9.22 (1999): 1351–1354. Print.