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Zebrafish collagen type I: molecular and biochemical characterization of the major structural protein in bone and skin

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Abstract
Over the last years the zebrafish imposed itself as a powerful model to study skeletal diseases, but a limit to its use is the poor characterization of collagen type I, the most abundant protein in bone and skin. In tetrapods collagen type I is a trimer mainly composed of two alpha 1 chains and one alpha 2 chain, encoded by COL1A1 and COL1A2 genes, respectively. In contrast, in zebrafish three type I collagen genes exist, col1a1a, col1a1b and col1a2 coding for alpha 1(I), alpha 3(I) and alpha 2(I) chains. During embryonic and larval development the three collagen type I genes showed a similar spatio-temporal expression pattern, indicating their co-regulation and interdependence at these stages. In both embryonic and adult tissues, the presence of the three a(I) chains was demonstrated, although in embryos alpha 1(I) was present in two distinct glycosylated states, suggesting a developmental-specific collagen composition. Even though in adult bone, skin and scales equal amounts of alpha 1(I), alpha 3(I) and alpha 2(I) chains are present, the presented data suggest a tissue-specific stoichiometry and/or post-translational modification status for collagen type I. In conclusion, this data will be useful to properly interpret results and insights gained from zebrafish models of skeletal diseases.
Keywords
ALPHA-CHAINS, FISH, WIDE DISTRIBUTION, 2-DIMENSIONAL ELECTROPHORESIS, DANIO-RERIO, OSTEOGENESIS IMPERFECTA, PROCOLLAGEN, STABILITY, PERSPECTIVES, IDENTIFICATION

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Chicago
Gistelinck, Charlotte, R Gioia, A Gagliardi, F Tonelli, L Marchese, L Bianchi, C Landi, et al. 2016. “Zebrafish Collagen Type I: Molecular and Biochemical Characterization of the Major Structural Protein in Bone and Skin.” Scientific Reports 6.
APA
Gistelinck, C., Gioia, R., Gagliardi, A., Tonelli, F., Marchese, L., Bianchi, L., Landi, C., et al. (2016). Zebrafish collagen type I: molecular and biochemical characterization of the major structural protein in bone and skin. SCIENTIFIC REPORTS, 6.
Vancouver
1.
Gistelinck C, Gioia R, Gagliardi A, Tonelli F, Marchese L, Bianchi L, et al. Zebrafish collagen type I: molecular and biochemical characterization of the major structural protein in bone and skin. SCIENTIFIC REPORTS. 2016;6.
MLA
Gistelinck, Charlotte et al. “Zebrafish Collagen Type I: Molecular and Biochemical Characterization of the Major Structural Protein in Bone and Skin.” SCIENTIFIC REPORTS 6 (2016): n. pag. Print.
@article{7901507,
  abstract     = {Over the last years the zebrafish imposed itself as a powerful model to study skeletal diseases, but a limit to its use is the poor characterization of collagen type I, the most abundant protein in bone and skin. In tetrapods collagen type I is a trimer mainly composed of two alpha 1 chains and one alpha 2 chain, encoded by COL1A1 and COL1A2 genes, respectively. In contrast, in zebrafish three type I collagen genes exist, col1a1a, col1a1b and col1a2 coding for alpha 1(I), alpha 3(I) and alpha 2(I) chains. During embryonic and larval development the three collagen type I genes showed a similar spatio-temporal expression pattern, indicating their co-regulation and interdependence at these stages. In both embryonic and adult tissues, the presence of the three a(I) chains was demonstrated, although in embryos alpha 1(I) was present in two distinct glycosylated states, suggesting a developmental-specific collagen composition. Even though in adult bone, skin and scales equal amounts of alpha 1(I), alpha 3(I) and alpha 2(I) chains are present, the presented data suggest a tissue-specific stoichiometry and/or post-translational modification status for collagen type I. In conclusion, this data will be useful to properly interpret results and insights gained from zebrafish models of skeletal diseases.},
  articleno    = {21540},
  author       = {Gistelinck, Charlotte and Gioia, R and Gagliardi, A and Tonelli, F and Marchese, L and Bianchi, L and Landi, C and Bini, L and Huysseune, Ann and Witten, Paul and Staes, An and Gevaert, Kris and De Rocker, Nina and Menten, Björn and Malfait, Fransiska and Leikin, S and Carra, S and Tenni, R and Rossi, A and De Paepe, Anne and Coucke, Paul and Willaert, Andy and Forlino, A},
  issn         = {2045-2322},
  journal      = {SCIENTIFIC REPORTS},
  keywords     = {ALPHA-CHAINS,FISH,WIDE DISTRIBUTION,2-DIMENSIONAL ELECTROPHORESIS,DANIO-RERIO,OSTEOGENESIS IMPERFECTA,PROCOLLAGEN,STABILITY,PERSPECTIVES,IDENTIFICATION},
  language     = {eng},
  pages        = {14},
  title        = {Zebrafish collagen type I: molecular and biochemical characterization of the major structural protein in bone and skin},
  url          = {http://dx.doi.org/10.1038/srep21540},
  volume       = {6},
  year         = {2016},
}

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