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High oxygen tension increases global methylation in bovine 4-cell embryos and blastocysts but does not affect general retrotransposon expression

Wenwen Li (UGent) , Karen Goossens, Mario Van Poucke (UGent) , Katrien Forier (UGent) , Kevin Braeckmans (UGent) , Ann Van Soom (UGent) and Luc Peelman (UGent)
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Center for nano- and biophotonics (NB-Photonics)
Abstract
Retrotransposons are transposable elements that insert extra copies of themselves throughout the genome via an RNA intermediate using a copy and paste' mechanism. They account for more than 44% of the bovine genome and have been reported to be functional, especially during preimplantation embryo development. In the present study, we tested whether high oxygen tension (20% O-2) influences global DNA methylation analysed by immunofluorescence staining of developing bovine embryos and whether this has an effect on the expression of some selected retrotransposon families. High oxygen tension significantly increased global DNA methylation in 4-cell embryos and blastocysts. A significant expression difference was observed for ERV1-1-I_BT in female blastocysts, but no significant changes were observed for the other retrotransposon families tested. Therefore, the study indicates that global DNA methylation is not necessarily correlated with retrotransposon expression in bovine preimplantation embryos.
Keywords
X-CHROMOSOME INACTIVATION, CULTURED IN-VITRO, SMALL INTERFERING RNAS, CELL NUCLEAR TRANSFER, REAL-TIME PCR, DNA METHYLATION, PREIMPLANTATION EMBRYOS, L1 RETROTRANSPOSITION, MAMMALIAN DEVELOPMENT, MOUSE OOCYTES, reference genes, oxidative stress, bovine preimplantation embryo, DNA methylation

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Citation

Please use this url to cite or link to this publication:

Chicago
Li, Wenwen, Karen Goossens, Mario Van Poucke, Katrien Forier, Kevin Braeckmans, Ann Van Soom, and Luc Peelman. 2016. “High Oxygen Tension Increases Global Methylation in Bovine 4-cell Embryos and Blastocysts but Does Not Affect General Retrotransposon Expression.” Reproduction Fertility and Development 28 (7): 948–959.
APA
Li, Wenwen, Goossens, K., Van Poucke, M., Forier, K., Braeckmans, K., Van Soom, A., & Peelman, L. (2016). High oxygen tension increases global methylation in bovine 4-cell embryos and blastocysts but does not affect general retrotransposon expression. REPRODUCTION FERTILITY AND DEVELOPMENT, 28(7), 948–959.
Vancouver
1.
Li W, Goossens K, Van Poucke M, Forier K, Braeckmans K, Van Soom A, et al. High oxygen tension increases global methylation in bovine 4-cell embryos and blastocysts but does not affect general retrotransposon expression. REPRODUCTION FERTILITY AND DEVELOPMENT. 2016;28(7):948–59.
MLA
Li, Wenwen, Karen Goossens, Mario Van Poucke, et al. “High Oxygen Tension Increases Global Methylation in Bovine 4-cell Embryos and Blastocysts but Does Not Affect General Retrotransposon Expression.” REPRODUCTION FERTILITY AND DEVELOPMENT 28.7 (2016): 948–959. Print.
@article{7898416,
  abstract     = {Retrotransposons are transposable elements that insert extra copies of themselves throughout the genome via an RNA intermediate using a copy and paste' mechanism. They account for more than 44\% of the bovine genome and have been reported to be functional, especially during preimplantation embryo development. In the present study, we tested whether high oxygen tension (20\% O-2) influences global DNA methylation analysed by immunofluorescence staining of developing bovine embryos and whether this has an effect on the expression of some selected retrotransposon families. High oxygen tension significantly increased global DNA methylation in 4-cell embryos and blastocysts. A significant expression difference was observed for ERV1-1-I\_BT in female blastocysts, but no significant changes were observed for the other retrotransposon families tested. Therefore, the study indicates that global DNA methylation is not necessarily correlated with retrotransposon expression in bovine preimplantation embryos.},
  author       = {Li, Wenwen and Goossens, Karen and Van Poucke, Mario and Forier, Katrien and Braeckmans, Kevin and Van Soom, Ann and Peelman, Luc},
  issn         = {1031-3613},
  journal      = {REPRODUCTION FERTILITY AND DEVELOPMENT},
  keyword      = {X-CHROMOSOME INACTIVATION,CULTURED IN-VITRO,SMALL INTERFERING RNAS,CELL NUCLEAR TRANSFER,REAL-TIME PCR,DNA METHYLATION,PREIMPLANTATION EMBRYOS,L1 RETROTRANSPOSITION,MAMMALIAN DEVELOPMENT,MOUSE OOCYTES,reference genes,oxidative stress,bovine preimplantation embryo,DNA methylation},
  language     = {eng},
  number       = {7},
  pages        = {948--959},
  title        = {High oxygen tension increases global methylation in bovine 4-cell embryos and blastocysts but does not affect general retrotransposon expression},
  url          = {http://dx.doi.org/10.1071/RD14133},
  volume       = {28},
  year         = {2016},
}

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