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Generation of a new Gateway-compatible inducible lentiviral vector platform allowing easy derivation of co-transduced cells

Philippe De Groote (UGent) , Sasker Grootjans (UGent) , Saskia Lippens (UGent) , Chantal Eichperger (UGent) , Kirsten Leurs (UGent) , Irene Kahr (UGent) , Giel Tanghe (UGent) , Inge Bruggeman (UGent) , Wouter De Schamphelaire (UGent) , Corinne Urwyler (UGent) , et al.
(2016) BIOTECHNIQUES. 60(5). p.252-259
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Abstract
In contrast to most common gene delivery techniques, lentiviral vectors allow targeting of almost any mammalian cell type, even non-dividing cells, and they stably integrate in the genome. Therefore, these vectors are a very powerful tool for biomedical research. Here we report the generation of a versatile new set of 22 lentiviral vectors with broad applicability in multiple research areas. In contrast to previous systems, our platform provides a choice between constitutive and/or conditional expression and six different C-terminal fusions. Furthermore, two compatible selection markers enable the easy derivation of stable cell lines co-expressing differently tagged transgenes in a constitutive or inducible manner. We show that all of the vector features are functional and that they contribute to transgene overexpression in proof-of-principle experiments.
Keywords
NF-KAPPA-B, RNA INTERFERENCE, Gateway, co-inducible expression, Tet-On, MAMMALIAN-CELLS, LINE, KINASE, RECOMBINANT PROTEINS, GENE-EXPRESSION, IN-VIVO, SYSTEM, lentiviral vectors

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Chicago
De Groote, Philippe, Sasker Grootjans, Saskia Lippens, Chantal Eichperger, Kirsten Leurs, Irene Kahr, Giel Tanghe, et al. 2016. “Generation of a New Gateway-compatible Inducible Lentiviral Vector Platform Allowing Easy Derivation of Co-transduced Cells.” Biotechniques 60 (5): 252–259.
APA
De Groote, P., Grootjans, S., Lippens, S., Eichperger, C., Leurs, K., Kahr, I., Tanghe, G., et al. (2016). Generation of a new Gateway-compatible inducible lentiviral vector platform allowing easy derivation of co-transduced cells. BIOTECHNIQUES, 60(5), 252–259.
Vancouver
1.
De Groote P, Grootjans S, Lippens S, Eichperger C, Leurs K, Kahr I, et al. Generation of a new Gateway-compatible inducible lentiviral vector platform allowing easy derivation of co-transduced cells. BIOTECHNIQUES. 2016;60(5):252–9.
MLA
De Groote, Philippe, Sasker Grootjans, Saskia Lippens, et al. “Generation of a New Gateway-compatible Inducible Lentiviral Vector Platform Allowing Easy Derivation of Co-transduced Cells.” BIOTECHNIQUES 60.5 (2016): 252–259. Print.
@article{7898379,
  abstract     = {In contrast to most common gene delivery techniques, lentiviral vectors allow targeting of almost any mammalian cell type, even non-dividing cells, and they stably integrate in the genome. Therefore, these vectors are a very powerful tool for biomedical research. Here we report the generation of a versatile new set of 22 lentiviral vectors with broad applicability in multiple research areas. In contrast to previous systems, our platform provides a choice between constitutive and/or conditional expression and six different C-terminal fusions. Furthermore, two compatible selection markers enable the easy derivation of stable cell lines co-expressing differently tagged transgenes in a constitutive or inducible manner. We show that all of the vector features are functional and that they contribute to transgene overexpression in proof-of-principle experiments.},
  author       = {De Groote, Philippe and Grootjans, Sasker and Lippens, Saskia and Eichperger, Chantal and Leurs, Kirsten and Kahr, Irene and Tanghe, Giel and Bruggeman, Inge and De Schamphelaire, Wouter and Urwyler, Corinne and Vandenabeele, Peter and Haustraete, Jurgen and Declercq, Wim},
  issn         = {0736-6205},
  journal      = {BIOTECHNIQUES},
  keyword      = {NF-KAPPA-B,RNA INTERFERENCE,Gateway,co-inducible expression,Tet-On,MAMMALIAN-CELLS,LINE,KINASE,RECOMBINANT PROTEINS,GENE-EXPRESSION,IN-VIVO,SYSTEM,lentiviral vectors},
  language     = {eng},
  number       = {5},
  pages        = {252--259},
  title        = {Generation of a new Gateway-compatible inducible lentiviral vector platform allowing easy derivation of co-transduced cells},
  url          = {http://dx.doi.org/10.2144/000114417},
  volume       = {60},
  year         = {2016},
}

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