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Generation of a new Gateway-compatible inducible lentiviral vector platform allowing easy derivation of co-transduced cells

Philippe De Groote UGent, Sasker Grootjans, Saskia Lippens UGent, Chantal Eichperger UGent, Kirsten Leurs UGent, Irene Kahr UGent, Giel Tanghe UGent, Inge Bruggeman UGent, Wouter De Schamphelaire UGent, Corinne Urwyler, et al. (2016) BIOTECHNIQUES. 60(5). p.252-259
abstract
In contrast to most common gene delivery techniques, lentiviral vectors allow targeting of almost any mammalian cell type, even non-dividing cells, and they stably integrate in the genome. Therefore, these vectors are a very powerful tool for biomedical research. Here we report the generation of a versatile new set of 22 lentiviral vectors with broad applicability in multiple research areas. In contrast to previous systems, our platform provides a choice between constitutive and/or conditional expression and six different C-terminal fusions. Furthermore, two compatible selection markers enable the easy derivation of stable cell lines co-expressing differently tagged transgenes in a constitutive or inducible manner. We show that all of the vector features are functional and that they contribute to transgene overexpression in proof-of-principle experiments.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
NF-KAPPA-B, RNA INTERFERENCE, Gateway, co-inducible expression, Tet-On, MAMMALIAN-CELLS, LINE, KINASE, RECOMBINANT PROTEINS, GENE-EXPRESSION, IN-VIVO, SYSTEM, lentiviral vectors
journal title
BIOTECHNIQUES
Biotechniques
volume
60
issue
5
pages
252 - 259
Web of Science type
Article
Web of Science id
000376419900005
JCR category
BIOCHEMICAL RESEARCH METHODS
JCR impact factor
2.03 (2016)
JCR rank
50/77 (2016)
JCR quartile
3 (2016)
ISSN
0736-6205
DOI
10.2144/000114417
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
7898379
handle
http://hdl.handle.net/1854/LU-7898379
date created
2016-06-28 12:07:09
date last changed
2016-12-19 15:42:57
@article{7898379,
  abstract     = {In contrast to most common gene delivery techniques, lentiviral vectors allow targeting of almost any mammalian cell type, even non-dividing cells, and they stably integrate in the genome. Therefore, these vectors are a very powerful tool for biomedical research. Here we report the generation of a versatile new set of 22 lentiviral vectors with broad applicability in multiple research areas. In contrast to previous systems, our platform provides a choice between constitutive and/or conditional expression and six different C-terminal fusions. Furthermore, two compatible selection markers enable the easy derivation of stable cell lines co-expressing differently tagged transgenes in a constitutive or inducible manner. We show that all of the vector features are functional and that they contribute to transgene overexpression in proof-of-principle experiments.},
  author       = {De Groote, Philippe and Grootjans, Sasker and Lippens, Saskia and Eichperger, Chantal and Leurs, Kirsten and Kahr, Irene and Tanghe, Giel and Bruggeman, Inge and De Schamphelaire, Wouter and Urwyler, Corinne and Vandenabeele, Peter and Haustraete, Jurgen and Declercq, Wim},
  issn         = {0736-6205},
  journal      = {BIOTECHNIQUES},
  keyword      = {NF-KAPPA-B,RNA INTERFERENCE,Gateway,co-inducible expression,Tet-On,MAMMALIAN-CELLS,LINE,KINASE,RECOMBINANT PROTEINS,GENE-EXPRESSION,IN-VIVO,SYSTEM,lentiviral vectors},
  language     = {eng},
  number       = {5},
  pages        = {252--259},
  title        = {Generation of a new Gateway-compatible inducible lentiviral vector platform allowing easy derivation of co-transduced cells},
  url          = {http://dx.doi.org/10.2144/000114417},
  volume       = {60},
  year         = {2016},
}

Chicago
De Groote, Philippe, Sasker Grootjans, Saskia Lippens, Chantal Eichperger, Kirsten Leurs, Irene Kahr, Giel Tanghe, et al. 2016. “Generation of a New Gateway-compatible Inducible Lentiviral Vector Platform Allowing Easy Derivation of Co-transduced Cells.” Biotechniques 60 (5): 252–259.
APA
De Groote, P., Grootjans, S., Lippens, S., Eichperger, C., Leurs, K., Kahr, I., Tanghe, G., et al. (2016). Generation of a new Gateway-compatible inducible lentiviral vector platform allowing easy derivation of co-transduced cells. BIOTECHNIQUES, 60(5), 252–259.
Vancouver
1.
De Groote P, Grootjans S, Lippens S, Eichperger C, Leurs K, Kahr I, et al. Generation of a new Gateway-compatible inducible lentiviral vector platform allowing easy derivation of co-transduced cells. BIOTECHNIQUES. 2016;60(5):252–9.
MLA
De Groote, Philippe, Sasker Grootjans, Saskia Lippens, et al. “Generation of a New Gateway-compatible Inducible Lentiviral Vector Platform Allowing Easy Derivation of Co-transduced Cells.” BIOTECHNIQUES 60.5 (2016): 252–259. Print.