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Suppression of Interleukin-33 Bioactivity through Proteolysis by Apoptotic Caspases

(2009) IMMUNITY. 31(1). p.84-98
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Abstract
Interleukin-33 (IL-33) is amember of the IL-1 family and is involved in polarization of T cells toward a T helper 2 (Th2) cell phenotype. IL-33 is thought to be activated via caspase-1-dependent proteolysis, similar to the proinflammatory cytokines IL-1b and IL-18, but this remains unproven. Here we showed that IL-33 was processed by caspases activated during apoptosis (caspase-3 and -7) but was not a physiological substrate for caspases associated with inflammation (caspase-1, -4, and -5). Furthermore, caspase-dependent processing of IL-33 was not required for ST2 receptor binding or ST2-dependent activation of the NF-kB transcription factor. Indeed, caspase-dependent proteolysis of IL-33 dramatically attenuated IL- 33 bioactivity in vitro and in vivo. These data suggest that IL-33 does not require proteolysis for activation, but rather, that IL-33 bioactivity is diminished through caspase-dependentproteolysis within apoptotic cells. Thus, caspase-mediated proteolysis acts as a switch to dampen the proinflammatory properties of IL-33.
Keywords
CYTOKINE, EXPRESSION, ST2, ACTIVATION, INNATE IMMUNE-SYSTEM, RECEPTOR ACCESSORY PROTEIN, T-CELLS, INFLAMMATORY CASPASES, IL-33, MAST-CELLS

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Citation

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MLA
Luthi, AU et al. “Suppression of Interleukin-33 Bioactivity Through Proteolysis by Apoptotic Caspases.” IMMUNITY 31.1 (2009): 84–98. Print.
APA
Luthi, A., Cullen, S., McNeela, E., Duriez, P., Afonina, I., Sheridan, C., Brumatti, G., et al. (2009). Suppression of Interleukin-33 Bioactivity through Proteolysis by Apoptotic Caspases. IMMUNITY, 31(1), 84–98.
Chicago author-date
Luthi, AU, SP Cullen, EA McNeela, PJ Duriez, IS Afonina, C Sheridan, G Brumatti, et al. 2009. “Suppression of Interleukin-33 Bioactivity Through Proteolysis by Apoptotic Caspases.” Immunity 31 (1): 84–98.
Chicago author-date (all authors)
Luthi, AU, SP Cullen, EA McNeela, PJ Duriez, IS Afonina, C Sheridan, G Brumatti, RC Taylor, Kristof Kersse, Peter Vandenabeele, EC Lavelle, and SJ Martin. 2009. “Suppression of Interleukin-33 Bioactivity Through Proteolysis by Apoptotic Caspases.” Immunity 31 (1): 84–98.
Vancouver
1.
Luthi A, Cullen S, McNeela E, Duriez P, Afonina I, Sheridan C, et al. Suppression of Interleukin-33 Bioactivity through Proteolysis by Apoptotic Caspases. IMMUNITY. CAMBRIDGE: CELL PRESS; 2009;31(1):84–98.
IEEE
[1]
A. Luthi et al., “Suppression of Interleukin-33 Bioactivity through Proteolysis by Apoptotic Caspases,” IMMUNITY, vol. 31, no. 1, pp. 84–98, 2009.
@article{787328,
  abstract     = {Interleukin-33 (IL-33) is amember of the IL-1 family and
is involved in polarization of T cells toward a T helper 2
(Th2) cell phenotype. IL-33 is thought to be activated
via caspase-1-dependent proteolysis, similar to the
proinflammatory cytokines IL-1b and IL-18, but this
remains unproven. Here we showed that IL-33 was
processed by caspases activated during apoptosis
(caspase-3 and -7) but was not a physiological
substrate for caspases associated with inflammation
(caspase-1, -4, and -5). Furthermore, caspase-dependent
processing of IL-33 was not required for ST2
receptor binding or ST2-dependent activation of the
NF-kB transcription factor. Indeed, caspase-dependent
proteolysis of IL-33 dramatically attenuated IL-
33 bioactivity in vitro and in vivo. These data suggest
that IL-33 does not require proteolysis for activation,
but rather, that IL-33 bioactivity is diminished through
caspase-dependentproteolysis within apoptotic cells.
Thus, caspase-mediated proteolysis acts as a switch
to dampen the proinflammatory properties of IL-33.},
  author       = {Luthi, AU and Cullen, SP and McNeela, EA and Duriez, PJ and Afonina, IS and Sheridan, C and Brumatti, G and Taylor, RC and Kersse, Kristof and Vandenabeele, Peter and Lavelle, EC and Martin, SJ},
  issn         = {1074-7613},
  journal      = {IMMUNITY},
  keywords     = {CYTOKINE,EXPRESSION,ST2,ACTIVATION,INNATE IMMUNE-SYSTEM,RECEPTOR ACCESSORY PROTEIN,T-CELLS,INFLAMMATORY CASPASES,IL-33,MAST-CELLS},
  language     = {eng},
  number       = {1},
  pages        = {84--98},
  publisher    = {CELL PRESS},
  title        = {Suppression of Interleukin-33 Bioactivity through Proteolysis by Apoptotic Caspases},
  url          = {http://dx.doi.org/10.1016/j.immuni.2009.05.007},
  volume       = {31},
  year         = {2009},
}

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